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Changes in endogenous hormones and H2O2 burst during shoot organogenesis in TDZ-treated Saussurea involucrate explants

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Abstract

Thidiazuron [N-phenyl-N-(1, 2, 3-thidiazol-5-yl) urea, TDZ] treatment significantly improved shoot morphogenesis of Saussurea involucrata Kar. Et Kir (S. involucrata) leaf explants. The biochemical mechanisms underlying TDZ-induced shoot organogenesis were investigated by measuring endogenous plant growth hormones, H2O2, as well as the activities of superoxide dismutase (SOD) and catalase (CAT). The levels of endogenous gibberellic A3 (GA3) and zeatin (ZT) significantly increased in leaf explants subject to a 28-day treatment than the controls. However, extending exposure time to TDZ inhibited GA3 accumulation. At the same time, the SOD activity increased significantly until the 28th of TDZ treatment time and the CAT activity reduced simultaneously, which was closely linked with the significant increase in H2O2 concentrations in the explants. And there was a sharply promotion after the 35 day of culture time if the plant tissue was always in medium contained, which was in company with the cell activity decreased. We propose that a combination of increased GA3, ZT, and H2O2 concentration is the basis for the enhanced shoot morphogenesis in response to TDZ treatment. These results provide a starting point for an improved understanding of the biochemical mechanisms underlying TDZ-induced shoot organogenesis of S. involucrata.

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Acknowledgments

This work was supported by Special Fund for Agro-scientific Research in The Public Interest (Grant No. 201203062), Natural Science Foundation of China (Grant Nos. 31402133, 41303063, 31572665, 30970308), the China Postdoctoral Science Foundation (Grant No. 2016M592830), Special Fund for Scientific Research from Shaanxi Provincial Department of Education (Grant No. 14JK1725), and Project of Natural Science Foundation research project of Shaanxi Province (Grant No. 2016JM3002).

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Authors and Affiliations

  1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an, 710069, Shaanxi, China

    Bin Guo, Wei He, Yandi Wu, Yanping Fu & Yahui Wei

  2. Institute of Quality Standard & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, China

    Yan Zhao

  3. Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing, China

    Yan Zhao

  4. Xi’an Institute of Optics and Precision Mechanics (XIOPM), CAS, Xi’an, China

    Juan Guo

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  1. Bin Guo
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  2. Wei He
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  3. Yan Zhao
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  4. Yandi Wu
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  5. Yanping Fu
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  6. Juan Guo
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  7. Yahui Wei
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Correspondence to Yahui Wei.

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Bin Guo and Wei He have contributed equally to this work.

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Guo, B., He, W., Zhao, Y. et al. Changes in endogenous hormones and H2O2 burst during shoot organogenesis in TDZ-treated Saussurea involucrate explants. Plant Cell Tiss Organ Cult 128, 1–8 (2017). https://doi.org/10.1007/s11240-016-1069-3

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  • DOI: https://doi.org/10.1007/s11240-016-1069-3

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