Abstract
An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and 5.2 shoots per leaf explant were achieved when cultured on a medium containing 10 μM 6-benzylaminopurine (BAP) and 2.5 μM 1-naphthaleneacetic acid (NAA). Shoot organogenesis was improved further when the leaf explants were pre-incubated at low temperature, and 80.6% of shoot regeneration frequency was recorded with 9.3 shoots per leaf explant at 4°C by 5-day pretreatment period. Up to 87.0% of the regenerated shoots formed complete plantlets on a medium containing 2.5 μM indole-3-acetic acid (IAA) within 28 days, and 85.2% of the regenerated plantlets survived and grew vigorously in greenhouse condition. The phytochemical profile of the micropropagated plants was similar to that of wild plants. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahn I, Kim BD (1992) Transition of cellular oxidation–reduction status in accordance with somatic embryogenesis during the cultures of ginseng (Panax ginseng). J Kor Soc Hort Sci 33:231–235
Andersone U, Levinah G (2005) In vitro regeneration of mature Pinus sylvestris buds stored at freezing temperatures. Biol Plantarum 49:281–284
Atkin RK, Barton GE, Robinson DK (1973) Effect of root-growing temperature on growth substances in xylem exudate of Zea mays. J Exp Biol 24:475–487
Baker MC, Munoz-Fernandez N, Carter CD (1999) Improved sheet development and rooting from mature leaves and roots of Eryngium foetidum L., a food flavouring agent. Curr Sci 75:644–666
Dhar U, Joshi M (2005) Efficient plant regeneration protocol through callus for Saussurea obvallata (DC) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators. Plant Cell Rep 24:195–200
Fu CX, Jin ZP, Yang R, Wu FY, Zhao DX (2004) Establishment of Saussurea involucrata hairy roots culture and plantlet regeneration. Chin J Biotech 20:366–371
Fu CX, Xu YJ, Zhao DX, Ma FS (2005) A comparison between hairy root cultures and wild plants of Saussurea involucrata in phenylpropanoids production. Plant Cell Rep 24:750–754
Fu LG (1992) China plant red data book—Rare and endangered plants, vol 1 [M]. Chinese Science Press, Beijing, pp 234–235
Hou BK, Yu HM, Teng SY (1997) Effects of low temperature on induction and differentiation of wheat calluses. Plant Cell Tiss Org Cult 49:35–38
Huberman M, Riov J, Aloni B, Goren R (1997) Role of ethylene biosynthesis and auxin content and transport in high temperature-induced abscission of pepper reproductive organs. J Plant Growth Regul 16:129–135
Jia JM, Wu CF, Liu W, Yu H, Hao Y, Zheng JH, Ji YR (2005) Antiinflammatory and analgesic activities of the tissue culture of Saussurea involucrata. Biol Pharm Bull 28:1612–1614
Joshi M, Dhar U (2003) In vitro propagation of Saussurea obvallata (DC.) Edgew.—an endangered ethnoreligious medicinal herb of Himalaya. Plant Cell Rep 21:933–939
Knittel N, Escandon AS, Hahne G (1991) Plant regeneration at high frequency from mature sunflower cotyledons. Plant Sci 73:219–226
Li GH, Zhao RC (1989) Studies on pharmacological actions of Saussure involucrata Kar. et Kir. Acta Pharmaceut Sin 15:368–369
Chinese Pharmacopoeia (2005) Chemical Industry Press, Beijing, pp 36–37
Liu L, Xiao X, Zhang L (1985) Effect of the flavonoids from Saussurea involucrata on DNA synthesis of cancer cells. Lanzhou Univ Nat Sci 21:80–83
Liu CZ, Murch S, Jain JC, Saxena PK (2004) Goldenseal (Hydrastis canadensis L.): in vitro regeneration for germplasm conservation and elimination of heavy metal contamination. In Vitro Mol Dev Biol-Plant 70:73–79
Liu CZ, Murch S, El-Demerdash M, Saxena PK (2003) Regeneration of Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep 21:525–530
Merce B, Rosa MC, Javier P, Esther C, Teresa PM, Carmen M (2003) Relationship between peroxidase activity and organogenesis in Panax ginsengs calluses. Plant Cell Tiss Org Cult 73:37–44
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Murch S, Peiris SE, Liu CZ, Saxena PK (2004) In vitro conservation and propagation of medicinal plants. Biodiversity 5:19–24
Renu A, Sant SB (1989) In vitro propagation and low temperature storage of Saussurea lappa C.B. Clarke—an endangered, medicinal plant. Plant Cell Rep 8:44–47
Singh R, Srivastava K, Jaiswal HK, Amla DV, Singh BD (2002) High frequency multiple shoot regeneration from decapitated embryo axes of chickpea and establishment of plantlets in the open environment. Biol Plantarum 45:505–508
Stfaan P, Werbrouck O, Debergh PC (1994) Applied aspects of plant regeneration. In: Dixon RA, Gonzales RA (eds) Plant cell culture: a practical approach. Oxford University Press, Oxford, pp 127–145
Acknowledgments
The authors acknowledge the financial support from the “Hundred Talents Program” of the Chinese Academy of Sciences.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by M. S. Petersen
Rights and permissions
About this article
Cite this article
Guo, B., Gao, M. & Liu, CZ. In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.. Plant Cell Rep 26, 261–265 (2007). https://doi.org/10.1007/s00299-006-0230-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00299-006-0230-6