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Thidiazuron-Induced Changes in Biomass Parameters, Total Phenolic Content, and Antioxidant Activity in Callus Cultures of Artemisia absinthium L.

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Abstract

Callus culture of Artemisia absinthium L. was established for enhanced production of phenolics and higher antioxidant activity. Callus was induced from seed-derived leaf explants, incubated on to MS media supplemented with thidiazuron (TDZ; 0.5–5.0 mg/l) either alone or in combination with α-naphthalene acetic acid (NAA; 1.0 mg/l). These callus cultures were investigated for their growth kinetics, total phenolic content, and antioxidant activity on weekly basis for a period of 49 days. Maximum dry biomass accumulation of 8.73 g/l was observed on day 42 in response to 1.0 mg/l TDZ and 1.0 mg/l NAA. Furthermore, maximum level of total phenolic content of 8.53 mg GAE/g DW and highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of 72.6 % were observed in calli formed in response to 1.0 mg/l TDZ on day 42. The results showed a positive correlation of total phenolic content and DPPH radical scavenging activity in most of the callus cultures of A. absinthium L.

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References

  1. Krebs, S., Omer, T. N., & Omer, B. (2010). Phytomedicine, 17(5), 305–309.

    Article  CAS  Google Scholar 

  2. Singh, R., Verma, P. K., & Singh, G. (2012). Journal of Intercultural Ethnopharmacology, 1(2), 101–104.

    Article  Google Scholar 

  3. Kordali, S., Kotan, R., Mavi, A., Cakir, A., Ala, A., & Yildirim, A. (2005). Journal of Agricultural and Food Chemistry, 53(24), 9452–9458.

    Article  CAS  Google Scholar 

  4. Canadanovic‐Brunet, J. M., Djilas, S. M., Cetkovic, G. S., & Tumbas, V. T. (2005). Journal of the Science of Food and Agriculture, 85(2), 265–272.

    Article  CAS  Google Scholar 

  5. Zhao, J., Davis, L. C., & Verpoorte, R. (2005). Biotechnology Advances, 23(4), 283–333.

    Article  CAS  Google Scholar 

  6. Cieśla, L., Kowalska, I., Oleszek, W., & Stochmal, A. (2013). Phytochemical Analysis, 24(1), 47–52.

    Article  CAS  Google Scholar 

  7. Lai, H., & Singh, N. P. (2006). Cancer Letters, 231(1), 43–48.

    Article  CAS  Google Scholar 

  8. Sun, J., Chu, Y. F., Wu, X., & Liu, R. H. (2002). Journal of Agricultural and Food Chemistry, 50(25), 7449–7454.

    Article  CAS  Google Scholar 

  9. Rice-evans, C. A., Miller, N. J., Bolwell, P. G., Bramley, P. M., & Pridham, J. B. (1995). Free Radical Research, 22(4), 375–383.

    Article  CAS  Google Scholar 

  10. Hussain, M. S., Fareed, S., Saba Ansari, M., Rahman, A., Ahmad, I. Z., & Saeed, M. (2012). Journal of Pharmacy & Bio Allied Sciences, 4(1), 10.

    Article  Google Scholar 

  11. Murashige, T., & Skoog, F. (1962). Physiologia Plantarum, 15(3), 473–497.

    Article  CAS  Google Scholar 

  12. Ali, M., Abbasi, B. H., & Ihsan-ul-haq, A. (2013). Industrial Crops and Products, 49, 400–406.

    Article  CAS  Google Scholar 

  13. Velioglu, Y. S., Mazza, G., Gao, L., & Oomah, B. D. (1998). Journal of Agricultural and Food Chemistry, 46(10), 4113–4117.

    Article  CAS  Google Scholar 

  14. Abbasi, B. H., Khan, M. A., Mahmood, T., Ahmad, M., Chaudhary, M. F., & Khan, M. A. (2010). Plant Cell Tissue and Organ Culture (PCTOC), 101(3), 371–376.

    Article  CAS  Google Scholar 

  15. Nin, S., Bennici, A., Roselli, G., Mariotti, D., Schiff, S., & Magherini, R. (1997). Plant Cell Reports, 16(10), 725–730.

    Article  CAS  Google Scholar 

  16. Zia, M., Mannan, A., & Chaudhary, M. F. (2007). Pakistan Journal of Botany, 39.

  17. Zia, M., Rehman, R., & Chaudhary, M. F. (2007). African Journal of Biotechnology, 6(16).

  18. Rasool, R., Ganai, B. A., Kamili, A. N., & Akbar, S. (2012). Natural Product Research, 26(22), 2103–2106.

    CAS  Google Scholar 

  19. Danya, U., Udhayasankar, M. R., Punitha, D., Arumugasamy, K., & Suresh, S. N. (2012). International Journal of Plant, Animal and Environmental Sciences, 2(4).

  20. Abbasi, B. H., Khan, M., Guo, B., Bokhari, S. A., & Khan, M. A. (2011). Plant Cell Tissue and Organ Culture (PCTOC), 105(3), 337–344.

    Article  CAS  Google Scholar 

  21. Erişen, S., Atalay, E., & Yorgancılar, M. (2011). Turkish Journal of Botany, 35, 521–526.

    Google Scholar 

  22. Yorgancilar, M., & Erisen, S. (2011). Journal of Animal and Plant Sciences, 21.

  23. Huan, L. V. T., Takamura, T., & Tanaka, M. (2004). Plant Science, 166(6), 1443–1449.

    Article  CAS  Google Scholar 

  24. Murthy, B. N. S., Murch, S. J., & Saxena, P. K. (1998). In Vitro Cellular & Developmental Biology-Plant, 34(4), 267–275.

    Article  CAS  Google Scholar 

  25. Schulze, J. (2007). Fruit Vegetable Cereal Sci Biotechnol, 1, 64–79.

    Google Scholar 

  26. Huetteman, C. A., & Preece, J. E. (1993). Plant Cell, Tissue and Organ Culture, 33(2), 105–119.

    Article  CAS  Google Scholar 

  27. Chen, X. Y., Ye, Q. S., & Liu, W. (2003). Subtropical Plant Science, 3, 015.

    Google Scholar 

  28. Nabila, S. K., Fawzia, M. J., Naser, A. A., & Rida, A. S. (2003). Plant cell, Tissue and Organ Culture, 73(2), 117–121.

    Article  Google Scholar 

  29. Jayasinghe, C., Gotoh, N., Aoki, T., & Wada, S. (2003). Journal of Agricultural and Food Chemistry, 51(15), 4442–4449.

    Article  CAS  Google Scholar 

  30. Ali, M. B., Khatun, S., Hahn, E. J., & Paek, K. Y. (2006). Plant Growth Regulation, 49(2–3), 137–146.

    Article  CAS  Google Scholar 

  31. Kim, H. J., Chen, F., Wang, X., & Choi, J. H. (2006). Journal of Agricultural and Food Chemistry, 54(19), 7263–7269.

    Article  CAS  Google Scholar 

  32. Ali, M. B., Hahn, E. J., & Paek, K. Y. (2007). Molecules, 12(3), 607–621.

    Article  CAS  Google Scholar 

  33. Roby, M. H. H., Sarhan, M. A., Selim, K. A., & Khalel, K. I. (2013). Industrial Crops and Products, 43, 827–831.

    Article  CAS  Google Scholar 

  34. Schmeda-Hirschmann, G., Jordan, M., Gerth, A., & Wilken, D. (2005). Zeitschrift für Naturforschung, 60(1–2), 5–10.

    CAS  Google Scholar 

  35. Naz, S., Ali, A., & Iqbal, J. (2008). Pakistan Journal of Botany, 40(6), 2525–2539.

    CAS  Google Scholar 

  36. Giri, L., Dhyani, P., Rawat, S., Bhatt, I. D., Nandi, S. K., Rawal, R. S., & Pande, V. (2012). Industrial Crops and Products, 39, 1–6.

    Article  CAS  Google Scholar 

  37. Amid, A., Johan, N. N., Jamal, P., & Zain, W. N. W. M. (2011). African Journal of Biotechnology, 10(81), 18653–18656.

    CAS  Google Scholar 

  38. Al Khateeb, W., Hussein, E., Qouta, L., Aludatt, M., Al-Shara, B., & Abu-zaiton, A. (2012). Plant Cell Tissue and Organ Culture (PCTOC), 110(1), 103–110.

    Article  CAS  Google Scholar 

  39. Diwan, R., Shinde, A., & Malpathak, N. (2012). Journal of Botany. doi:10.1155/2012/685427.

    Google Scholar 

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Acknowledgments

Financial support from Higher Education Commission (HEC) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    Mohammad Ali & Bilal Haider Abbasi

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  1. Mohammad Ali
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  2. Bilal Haider Abbasi
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Correspondence to Bilal Haider Abbasi.

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Ali, M., Abbasi, B.H. Thidiazuron-Induced Changes in Biomass Parameters, Total Phenolic Content, and Antioxidant Activity in Callus Cultures of Artemisia absinthium L.. Appl Biochem Biotechnol 172, 2363–2376 (2014). https://doi.org/10.1007/s12010-013-0663-7

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