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Molecular characterization of PeNhaD1: the first member of the NhaD Na+/H+ antiporter family of plant origin

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Abstract

PeNhaD1 encodes a putative Na+/H+ antiporter from the salt-resistant tree Populus euphratica. It is the first characterization of a member of the NhaD type ion transporter family of plant origin. Homology searches revealed its close relation to functionally characterized microbial Na+/H+ antiporters VpNhaD and VcNhaD. Na+/H+ antiporters have proven to play a key role in salt resistance, both in plants and bacteria. Under salt stress transcript levels of PeNhaD1 were maintained only in the salt-resistant P. euphratica, but collapsed in Populus × canescens, a salt-sensitive species. To address the function of PeNhaD1, complementation studies with the salt-sensitive Escherichia coli EP432 mutant strain, lacking activity of the two Na+/H+ antiporters EcNhaA and EcNhaB were carried out. PeNhaD1 was able to restore growth of EP432 under stress imposed by up to 400 mM NaCl demonstrating its protective function. Growth rates of EP432 were always highest at pH 5.5 while growth was suppressed under salt stress at pH 7.0 and pH 8.0 suggesting that the antiporter activity is strongly pH dependent. Element analyses of EP432 cells complemented with PeNhaD1 growing under salt stress showed that salt resistance was correlated with a significant reduction in sodium accumulation. These results suggest that PeNhaD1 might play a role in the salt resistance of P. euphratica.

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Authors and Affiliations

  1. Institut für Forstbotanik, , Georg-August-Universität Göttingen, Büsgenweg 2, 37077, Göttingen, Germany

    Eric A. Ottow, Andrea Polle & Thomas Teichmann

  2. Plant Biology, Department of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland

    Mikael Brosché & Jaakko Kangasjärvi

  3. Department of Microbiology, Faculty of Science, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada

    Pavel Dibrov

  4. Institut für Pflanzenernährung, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany

    Christian Zörb

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  1. Eric A. Ottow
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  2. Andrea Polle
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  3. Mikael Brosché
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  4. Jaakko Kangasjärvi
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Correspondence to Thomas Teichmann.

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Ottow, E.A., Polle, A., Brosché, M. et al. Molecular characterization of PeNhaD1: the first member of the NhaD Na+/H+ antiporter family of plant origin. Plant Mol Biol 58, 75–88 (2005). https://doi.org/10.1007/s11103-005-4525-8

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