Skip to main content

Advertisement

SpringerLink
Log in

Axillary proliferation and tuberisation of Dioscorea cayenensis–D. rotundata complex

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Yams (Dioscorea spp) are tuber crops used as staple food in Africa because of their nutritional value. However agronomic constraints, phytosanitary problems and the lack of good healthy planting material restrict their production. In contrast to the inefficiency of traditional method of planting, tissue culture techniques allow to increase the multiplication and the rapid production of pathogen- free plant material. This work was undertaken to provide farmers in African countries with healthy microplants and microtubers as seeds. In vitro nodal segments of two varieties of local yams D. cayenensis–D. rotundata complex (cv. ‘Singo’, cv. ‘Singou’ and cv. ‘Gnidou’) were micropropagated on the modified medium of Murashige and Skoog. The morphogenesis, the growth of microplants and microtuber formation have been found to be controlled by external factors that act individually and synergistically. Addition of kinetin (2 mg l−1) to the culture media could reduce multiplication rate (node number) of some clones. An increase of the sucrose concentration from 3% to 5% induced no change in the multiplication and tuberisation parameters. An important reduction of the multiplication (shoot number, height and node number) and the tuberisation (tuber number and length) was observed with 8% sucrose. Multiplication (shoot and node number) was increased in the presence of jasmonic acid (10 μM). JA also induced an increase of tuber number in the absence of Kin. Multiplication of yam by in vitro growth of nodal segments is a way for rapid clonal multiplication and could allow solving the problem of lack of seed material faced by farmers. This method could also be used for multiplication of elite cultivars, independently of the growing season.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

JA:

Jasmonic acid

Kin:

Kinetin

MS:

Murashige and Skoog

References

  • Abdala G, Castro G, Guinazu MM et al (1996) Occurrence of jasmonic acid in organs of Solanum tuberosum L. and its effect on tuberisation. Plant Growth Regul 19:139–143

    Article  CAS  Google Scholar 

  • Alizadeh S, Mantell SH, Viana AM (1998) In vitro shoot culture and microtuber induction in the steroid yam Dioscorea composite Hemsl. Plant Cell Tiss Organ Cult 53:107–112

    Article  Google Scholar 

  • Ayensu ES, Coursey DG (1972) Guinea yams: the botany, ethno-botany, use and possible future of yams in West Africa. Econ Bot 26:301–318

    Google Scholar 

  • Balogun MO, Fawole I, Ng SYC et al (2006) Interaction among cultural factors in microtuberisation of white yam (Dioscorea rotundata). Trop Sci 46:55–59

    Article  Google Scholar 

  • Banfalvi Z, Molnar A, Kostyal Z et al (1997) Comparative studies on potato tuber development using an in vitro tuber induction system. Acta Biol Hungarica 48:77–86

    CAS  Google Scholar 

  • Bazabakana R, Baucher M, Diallo B, Jaziri M (2003) Effect of jasmonic acid on developmental morphology during in vitro tuberization of Dioscorea alata (L.). Plant Growth Regul 40:229–237

    Article  CAS  Google Scholar 

  • Borges M, Ceiro W, Meneses S et al (2004) Regeneration and multiplication of Dioscorea alata germplasm maintained in vitro. Plant Cell Tiss Organ Cult 76:87–90

    Article  CAS  Google Scholar 

  • Borthakur M, Singh RS (2002) Direct plantlet regeneration from male inflorescences of medicinal yam (Dioscorea floribunda Mart and Gal.). In Vitro Cell Dev Biol-Plant 38:183–185

    Article  CAS  Google Scholar 

  • Chen Y, Fan J, Yi F, Luo Z, Fu Y (2003) Rapid clonal propagation of Dioscorea zingiberensis. Plant Cell Tiss Organ Cult 73:75–80

    Article  CAS  Google Scholar 

  • Chu EP, Ribeiro RDLF (2002) Growth and carbohydrate changes in shoot cultures of Dioscorea species as influenced by photoperiod, exogenous sucrose and cytokinin concentrations. Plant Cell Tiss Organ Cult 70:241–249

    Article  CAS  Google Scholar 

  • Coursey DG (1967) Yams. Longmans, Green and Co. Ltd, London

    Google Scholar 

  • Craufurd PQ, Battey NH, Ile EI, Asedu R (2006) Phases of dormancy in yam tubers (Dioscorea rotundata). Ann Bot 97:497–504

    Article  PubMed  Google Scholar 

  • Debeljak N, Regvar M, Dixon KW, Sivasithamparam K (2002) Induction of tuberization in vitro with jasmonic acid and sucrose in an Australian terrestrial orchid, Pterostylis sanguinea. Plant Growth Regul 36:253–260

    Article  CAS  Google Scholar 

  • Forsyth C, Van Staden J (1984) Tuberization of Dioscorea bulbifera stem nodes in culture. J Plant Physiol 115:79–83

    CAS  Google Scholar 

  • Gaspar T, Kevers C, Penel C et al (1996) Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cell Dev Biol-Plant 32:272–289

    CAS  Google Scholar 

  • Gibson IS (2005) Control of development and gene expression by sugar signalling. Plant Biol 8:93–102

    CAS  Google Scholar 

  • Hahn SK (1995) Yams: Dioscorea spp. (Dioscoreaceae). In: Smart J, Simmonds NW (eds) Evolution of crop plants. Longman Scientific and Technical, London UK, pp 112–120

    Google Scholar 

  • Jackson SD (1999) Multiple signaling pathways control tuber induction in potato. Plant Physiol 119:1–8

    Article  PubMed  CAS  Google Scholar 

  • Jasik J, Mantell SH (2000) Effects of jasmonic acid and its methylester on in vitro microtuberisation of three food yam (Dioscorea) species. Plant Cell Rep 19:863–867

    Article  CAS  Google Scholar 

  • Jean M, Cappadocia M (1991) In vitro tuberization in Dioscorea alata L. Brazo Fuerte and Florido and D. abyssinica Hoch. Plant Cell Tiss Organ Cult 26:147–152

    Google Scholar 

  • Kadota M, Niimi Y (2004) Improvement of micropropagation of Japonese yam using liquid and gelled medium culture. Sci Hort 102:461–466

    Article  CAS  Google Scholar 

  • Koda Y (1997) Possible involvement of jasmonates in various morphogenic events. Physiol Plant 100:639–646

    Article  CAS  Google Scholar 

  • Koda Y, Kikuta Y (1991) Possible involvement of jasmonic acid in tuberization of yam plants. Plant Physiol 32:629–633

    CAS  Google Scholar 

  • Koda Y, Kikuta Y, Tazaki H et al (1991) Potato tuber inducing activities of jasmonic acid and related compounds. Phytochemistry 30:1435–1438

    Article  CAS  Google Scholar 

  • Koda Y, Takahashi K, Kikuta Y (1994) Involvement of jasmonic acid and related compounds in tuberisation of Jerusalem artichoke plants (Helianthus tuberosum L.). J Crop Sci 63:333–338

    CAS  Google Scholar 

  • Kouassi B, Diopoh J, Fournet B (1990) Soluble sugars from yam and changes during tuber storage. Phytochemistry 29:1069–1072

    Article  CAS  Google Scholar 

  • Malaurie B, Pungu O, Trouslot M-F (1995a) Effect of growth regulators concentrations on morphological development of meristem-tips in D. cayenensis–D. rotundata complex and D. praehensilis. Plant Cell Tiss Organ Cult 41:229–235

    Article  CAS  Google Scholar 

  • Malaurie B, Thouvenel J-C, Pungu O (1995b) Influence of meristem-tip size and location on morphological development in D. cayenensis–D. rotundata complex ‘Grosse Caille’ and one genotype of D. praehensilis. Plant Cell Tiss Organ Cult 42:215–218

    Article  Google Scholar 

  • Mantell SH, Hugo SA (1989) Effects of photoperiod, mineral medium strength, inorganic ammonium, sucrose and cytokinin on root, shoot and microtuber development in shoot cultures of Dioscorea alata L. and D. bulbifera L. yams. Plant Cell Tiss Organ Cult 16:23–37

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479

    Article  CAS  Google Scholar 

  • Ng SYC (1988) In vitro tuberization in white yam (Dioscorea rotundata Poir). Plant Cell Tiss Organ Cult 14:121–128

    Article  Google Scholar 

  • Nyochembeng LM, Stephen G (1998) Plant regeneration from cocoyam callus derived from shoots tips and petioles. Plant Cell Tiss Organ Cult 61:99–134

    Google Scholar 

  • Onwueme IC (1978) The tropical tuber crops: yam, cassava, sweet potato and cocoyam. Chichester: John Wiley & Sons, New York

    Google Scholar 

  • Passam HC (1995) Induction, storage and germination of microtubers derived from single-node cuttings of mature yam plants. Trop Sci 35:217–219

    Google Scholar 

  • Ravnikar M, Vilhar B, Gogala N (1992) Stimulatory effect of jasmonic acid on potato stem nodes and protoplast culture. J Plant Growth Regul 11:29–33

    Article  CAS  Google Scholar 

  • Ravnikar M, Zel J, Plaper I, Spacapan A (1993) Jasmonic acid stimulates shoots and bulb formation of garlic in vitro. J Plant Growth Regul 12:73–77

    Article  CAS  Google Scholar 

  • Saleil VL, Degras L, Jonard R (1990) Obtention de plantes indemnes du virus de la mosaique de l’igname (YMV) par la culture in vitro des apex chez l’igname américaine Dioscorea trifida L. Agronomie 10:605–615

    Article  Google Scholar 

  • Santos I, Salema R (2000) Promotion by jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L. Plant Growth Regul 30:133–138

    Article  CAS  Google Scholar 

  • Scarcelli N, Tostain S, Mariac C, Agbangla C, Da O, Berthaud J, Pham JL (2006) Genetic nature of yams (Dioscorea sp.) domesticated by farmers in Benin (West Africa). Genet Res Crop Evol 53:121–130

    Article  CAS  Google Scholar 

  • Sembdner G, Parthier B (1993) The biochemistry and the physiological and molecular actions of jasmonates. Ann Rev Plant Physiol 44:569–589

    Article  CAS  Google Scholar 

  • Takahashi K, Fujino K, Kikuta Y, Koda Y (1995) Involvement of the accumulation of sucrose and the synthesis of cell wall polysaccharides in the expansion of potato cells in response to jasmonic acid. Plant Sci 111:11–18

    Article  CAS  Google Scholar 

  • Tschannen AB, Escher F, Stamp P (2005) Post-harvest treatment of seed tubers with gibberellic acid and field performance of yam (Dioscorea cayenensis–rotundata) in Ivory Coast. Expl Agric 41:175–186

    Article  CAS  Google Scholar 

  • Zel J, Debeljak N, Ucman R, Ravnikar M (1997) The effect of jasmonic acid, sucrose and darkness on garlic (Allium sativum L. cv.ptujski jesenski) bulb formation in vitro. In Vitro Cell Dev Biol Plant 33:231–235

    Article  CAS  Google Scholar 

Download references

Acknowledgment

Dr Filloux from CIRAD for providing the material, Prof. P. du Jardin and A. Carlier for their advices on yam in vitro culture, GABON government for awarding scholarship and the skilful assistance of the APE personnel (provided to CEDEVIT by the government of Wallonia).

Author information

Authors and Affiliations

  1. Plant Molecular Biology and Biotechnology Unit, University of Liege, Sart Tilman, B-22, Liege, 4000, Belgium

    Paul Ondo Ovono, Claire Kevers & Jacques Dommes

Authors
  1. Paul Ondo Ovono
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Claire Kevers
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jacques Dommes
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Claire Kevers.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ondo Ovono, P., Kevers, C. & Dommes, J. Axillary proliferation and tuberisation of Dioscorea cayenensis–D. rotundata complex. Plant Cell Tiss Organ Cult 91, 107–114 (2007). https://doi.org/10.1007/s11240-007-9238-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-007-9238-z

Keywords

Search

Navigation