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Micropropagation, in vitro flowering, and tuberization in Brachystelma glabrum Hook.f., an endemic species

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Abstract

Brachystelma glabrum Hook.f. is an endemic plant species of Eastern Ghats, India. In this study, efficient protocols for in vitro micropropagation, flowering, and tuberization of this plant were developed. Sterilized shoot tip and nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different plant growth regulators (PGRs) and additives for shoot induction and multiplication. Both shoot tip and nodal explants showed the best response (90 and 100%, respectively) on MS medium supplemented with thidiazuron (TDZ) at 1.0 mg L−1. The microshoots multiplied best on MS + TDZ (1.0 mg L−1) in combination with α-naphthaleneacetic acid (NAA) at 0.5 mg L−1 and coconut water (CW) at 25%. The highest number of in vitro flowers (4.0 flowers per microshoot) was observed on MS medium supplemented with a combination of N6-benzyladenine (BA) and indole-3-butyric acid (IBA), each at 1.5 mg L−1. In vitro-derived shoots produced aerial tubers on MS + TDZ (2.0 mg L−1) + IBA (0.5 mg L−1) and basal tubers on MS + TDZ at 2.0 mg L−1. In vitro shoots were best rooted on half-strength (½) MS + NAA at 0.5 mg L−1. The rooted plantlets were successfully acclimatized in pots with 70% survival after a hardening period of 1 mo. This protocol provides an effective method for the conservation of this endemic plant species.

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Acknowledgements

The authors are thankful to Dr. S. Kaliamoorthy, the Botanical Survey of India, Southern Regional Centre, National Orchidarium, and Experimental Garden, Yercaud, Tamil Nadu, India, for help rendered during the field work; and Dr. G.V.S. Murthy, Head of the Office, Botanical Survey of India, Southern Regional Centre, Coimbatore, Tamil Nadu, India, for authentication of the plant species. The author Dr. M.V. Rao acknowledge the UGC for providing Emeritus Fellowship.

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Authors and Affiliations

  1. Department of Plant Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India

    S. Revathi Lakshmi, S. Parthibhan, N. Ahamed Sherif & M.V. Rao

  2. Department of Botany, Ayya Nadar Janaki Ammal College, Sivakasi, Tamil Nadu, 626 124, India

    S. Revathi Lakshmi

  3. Department of Industry University Collaboration, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India

    T. Senthil Kumar

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  1. S. Revathi Lakshmi
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  2. S. Parthibhan
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  3. N. Ahamed Sherif
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  4. T. Senthil Kumar
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  5. M.V. Rao
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Correspondence to M.V. Rao.

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Revathi Lakshmi, S., Parthibhan, S., Ahamed Sherif, N. et al. Micropropagation, in vitro flowering, and tuberization in Brachystelma glabrum Hook.f., an endemic species. In Vitro Cell.Dev.Biol.-Plant 53, 64–72 (2017). https://doi.org/10.1007/s11627-017-9803-z

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