Abstract
The effect of jasmonic acid (JA), in a wide range of concentrations (0.01–100 μM), on the development of potato plantlets (Solanum tuberosum L.cv. Vesna) was investigated in order to discriminate between physiological and supraoptimal effects of this growth regulator in vitro. Concentrations from 0.1–1 μM significantly increased the length of developed plants. Application of JA in these concentrations resulted in a very well differentiated root system with many lateral branches. With increasing JA concentrations, the main roots shortened and thickened. Concentrations higher than 10 μM led to the compaction of the stem, roots, and root hairs, giving a stunted appearance to the plants. The effect of JA on cell wall regeneration and callus formation was observed in potato leaf protoplast culture. JA at concentrations from 0.01–1 μM stimulated cell division and microcalli development.
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References
Andersen JM, Spilatro SR, Klauer SF, Franceschi VR (1989) Jasmonic acid-dependent increase in the level of vegetative storage proteins in soybean. Plant Sci 62:45–52
De Fossard RA (1976) Tissue culture for plant propagators. University of New England Printery, Australia, pp 95–131
Herrmann G, Lehmann J, Peterson A, Sembdner G, Weidhase RA, Parthier B (1989) Species and tissue specifity of jasmonate-induced abundant proteins. J Plant Physiol 134:703–709
Knöfel HD, Brückner C, Kramell R, Sembdner G, Schreiber K (1984) A radioimmunoassay for Jasmonic acid. Biochem Physiol Pflanzen 179:317–325
Meyer A, Miersch O, Büttner C, Dathe W, Sembdner G (1984) Occurrence of the plant growth regulator Jasmonic acid in plants. J Plant Growth Regul 3:1–8
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Parthier B (1990) Jasmonates: hormonal regulators or stress factors in leaf senescence? J Plant Growth Regul 9:57–63
Ravnikar M, Gogala N (1990) Regulation of potato meristem development by Jasmonic acid in vitro. J Plant Growth Regul 9:233–236
Ravnikar M, Gogala N, Benedičič D, Rode J (1990) The regulation of organogenesis with Jasmonic acid. Acta Hort 280:169–172
Sembdner G, Klose C (1985) (±)-Jasmonsaure—ein neues phytohormon? Biol Rdsch 23:29–40
Sembdner G, Herrmann G, Schliemann W (1989) V. Growth. Progress Botany 51:134–164
Shepard JF, Totten RE (1977) Mesophyll cell protoplasts of potato. Plant Physiol 60:313–316
Staswick P (1990) Novel regulation of vegetative storage protein genes. Plant Cell 2:1–6
Ueda J, Kato J (1982) Inhibition of cytokinin-induced plant growth by Jasmonic acid and its methyl ester. Physiol Plant 54:249–252
Vilhar B, Ravnikar M, Schara M, Nemec M, Gogala N, (1991) The influence of Jasmonic acid on biophysical properties of potato leaf protoplasts and roots. Plant Cell Reports 10:541–544
Weidhase RA, Kramell H, Lehmann J, Liebisch H, Lerbs W, Parthier B (1987) Methyljasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments. Plant Sci 51:177–186
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Ravnikar, M., Vilhar, B. & Gogala, N. Stimulatory effects of jasmonic acid on potato stem node and protoplast culture. J Plant Growth Regul 11, 29–33 (1992). https://doi.org/10.1007/BF00193840
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DOI: https://doi.org/10.1007/BF00193840