Abstract
In vitro morphological development of meristem-tips, excised from microshoots, is reported for one clone ‘Grosse Caille’ of D. cayenensis-D. rotundata complex and for one clone of D. praehensilis. The effects of size and location of meristem-tip on viability and morphological development after 3, 8 and 11 months in vitro culture were studied. The larger size of meristem-tip gave the best rooting and plantlet formation while the smaller size gave the best bud production. Axillary buds were more reactive than apical meristem-tips in callusing and rooting when small-sized meristems of the ‘Grosse Caille’ clone were used; the use of larger meristem-tips did not induce more differences between apical and axillary buds. The production of plantlets was successful, after 11 months of culture, with a score of 82 and 39 % survivors, for ‘Grosse Caille’ clone and D. praehensilis genotype, respectively.
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Abbreviations
- Ap:
-
apical meristem-tips
- ApLS:
-
large-sized apical meristem-tips
- Ax:
-
axillary meristem-tips
- AxLS:
-
large-sized axillary meristem-tips
- BA:
-
6-benzyladenine
- cv:
-
cultivar
- LS:
-
large-sized meristem-tip
- NAA:
-
naphthalenacetic acid
- SS:
-
small-sized meristem-tip
References
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Malaurie, B., Thouvenel, JC. & Pungu, O. Influence of meristem-tip size and location on morphological development in Dioscorea cayenensis-D. rotundata complex ‘Grosse Caille’ and one genotype of D. praehensilis . Plant Cell Tiss Organ Cult 42, 215–218 (1995). https://doi.org/10.1007/BF00034240
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DOI: https://doi.org/10.1007/BF00034240