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Synergistic effect of cytokinins and auxins enables mass clonal multiplication of drumstick tree (Moringa oleifera Lam.): a wonder

  • Micropropagation
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A Correction to this article was published on 19 February 2021

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Abstract

The synergistic effect of plant growth regulators on axillary bud proliferation for mass clonal multiplication of Moringa oleifera Lam. (vern. drumstick) has been assessed for the first time. Treatment of decoated seeds with 1% (w/v) Bavistin for 60 min, 0.33% (w/v) streptocycline for 30 min, and 0.1% (w/v) HgCl2 for 3.5 min resulted in complete removal of the surface contaminants. Maximum seed germination (89.13%) was obtained on quarter-strength Murashige & Skoog (MS) medium. Culture of nodal segments on MS + 6-benzyladenine (BA) at 3 mg L−1 resulted in multiple shoot proliferation with ~ 18 shoots per explant. All combinations of indole-3-acetic acid (IAA) + kinetin (Kn) resulted in elongated shoots, while only lower concentrations of BA (0.5 mg L−1), along with IAA (0.5 to 2 mg L−1), or Kn (0.5 to 5 mg L−1), showed significant synergy in the shoot morphogenesis. In addition, the maximum (100%) rooting efficiency was attained on half-strength MS medium supplemented with different concentrations of IAA and indole-3-butyric acid (IBA). The rooted plants were successfully established in the greenhouse for acclimatization. Clonality of the raised plants was assessed using 15 random primers of Operon® technologies (OPT and OPF series), and eight primers resulted in significant amplification with distinct, identical, and reproducible bands that confirmed clonality of the micropropagated plants. The present study provides a comprehensive analysis of the synergistic effect of plant growth regulators (PGRs) on in vitro shoot regeneration and proliferation for clonal mass multiplication disease-free plantlets, which can be utilized to maximize the yield of healthy and genetically identical plants of drumstick tree, which is considered to be a miracle multipurpose tree.

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Funding

Financial support in the form of seed grant no. EF/2017-18/QE04-03 from Manipal University Jaipur is gratefully acknowledged. Swati Gupta also thanks Manipal University Jaipur for providing the research fellowship.

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Authors and Affiliations

  1. Department of Biosciences, Manipal University Jaipur, Jaipur, India

    Swati Gupta & Rohit Jain

  2. Department of Botany, University of Rajasthan, Jaipur, India

    Sumita Kachhwaha

  3. Amity University Rajasthan, Jaipur, India

    Shanker Lal Kothari

Authors
  1. Swati Gupta
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  2. Sumita Kachhwaha
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  3. Shanker Lal Kothari
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  4. Rohit Jain
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Corresponding author

Correspondence to Rohit Jain.

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Editor: Wenhao Dai

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Gupta, S., Kachhwaha, S., Kothari, S.L. et al. Synergistic effect of cytokinins and auxins enables mass clonal multiplication of drumstick tree (Moringa oleifera Lam.): a wonder . In Vitro Cell.Dev.Biol.-Plant 56, 458–469 (2020). https://doi.org/10.1007/s11627-020-10065-0

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  • DOI: https://doi.org/10.1007/s11627-020-10065-0

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