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Co-transfer of LEA and bZip Genes from Tamarix Confers Additive Salt and Osmotic Stress Tolerance in Transgenic Tobacco

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Abstract

In this study, the additive effects of a late embryogenesis abundant (LEA) gene and a basic leucine zipper (bZIP) gene on salt and osmotic stress in Tamarix plants were analyzed. The constructs containing one or both of the LEA and bZIP genes were transformed into tobacco. Northern blot analysis showed the genes were overexpressed under the control of the CaMV 35S promoter in both dual and single gene-transgenic tobacco lines. The effects of salt and osmotic stress in transgenic tobacco plant were investigated. Following exposure to NaCl, mannitol, and PEG6000 stress, dual gene-transgenic lines showed higher seed generation and growth rates than single gene-transgenic lines and the wild-type. In response to NaCl stress, the dual gene-transgenic lines showed lower malondialdehyde and higher leaf chlorophyll content than single gene-transgenic lines and the wild-type. These results suggested that the co-expression of LEA and bZIP resulted in an additive enhancement of stress tolerance in dual gene-transgenic tobacco.

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Acknowledgments

This work was supported by the Genetically Modified Organisms Breeding Major Project (2009ZX08009-098B), the National Natural Science Foundation (31070595) of China, and the Fundamental Research Funds for the Central Universities (DL10EA01-02).

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), 26 Hexing Road, Harbin, 150040, China

    Guan-Zheng Qu, Lina Zang, Hu Xilin, Caiqiu Gao, Tangchun Zheng & Kai-Long Li

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  1. Guan-Zheng Qu
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  2. Lina Zang
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  3. Hu Xilin
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  4. Caiqiu Gao
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Correspondence to Kai-Long Li.

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Qu, GZ., Zang, L., Xilin, H. et al. Co-transfer of LEA and bZip Genes from Tamarix Confers Additive Salt and Osmotic Stress Tolerance in Transgenic Tobacco. Plant Mol Biol Rep 30, 512–518 (2012). https://doi.org/10.1007/s11105-011-0371-9

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