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Promoter diversity in multigene transformation

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Abstract

Multigene transformation (MGT) is becoming routine in plant biotechnology as researchers seek to generate more complex and ambitious phenotypes in transgenic plants. Every nuclear transgene requires its own promoter, so when coordinated expression is required, the introduction of multiple genes leads inevitably to two opposing strategies: different promoters may be used for each transgene, or the same promoter may be used over and over again. In the former case, there may be a shortage of different promoters with matching activities, but repetitious promoter use may in some cases have a negative impact on transgene stability and expression. Using illustrative case studies, we discuss promoter deployment strategies in transgenic plants that increase the likelihood of successful and stable multiple transgene expression.

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Acknowledgments

Work in our laboratory is supported by the Ministry of Science and Innovation, Spain (BFU2007-61413 and BIO2007-30738-E), a European Research Council Advanced Grant (BIOFORCE) to PC, and the CONSOLIDER Agrigenomics program funded by MICINN, Spain.

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Authors and Affiliations

  1. Departament de Producció Vegetal i Ciència Forestal, ETSEA, Universitat de Lleida, Av. Alcalde Rovira Roure 191, 25198, Lleida, Spain

    Ariadna Peremarti, Sonia Gómez-Galera, Shaista Naqvi, Gemma Farré, Maite Sabalza, Bruna Miralpeix, Svetlana Dashevskaya, Dawei Yuan, Koreen Ramessar, Paul Christou, Changfu Zhu, Ludovic Bassie & Teresa Capell

  2. Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

    Richard M. Twyman

  3. Institució Catalana de Recerca i Estudis Avançats, Bellaterra, Spain

    Paul Christou

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  1. Ariadna Peremarti
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  2. Richard M. Twyman
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  3. Sonia Gómez-Galera
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  11. Paul Christou
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  12. Changfu Zhu
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  14. Teresa Capell
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Correspondence to Paul Christou.

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Peremarti, A., Twyman, R.M., Gómez-Galera, S. et al. Promoter diversity in multigene transformation. Plant Mol Biol 73, 363–378 (2010). https://doi.org/10.1007/s11103-010-9628-1

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