Abstract
We report a strength comparison of a large variety of monocot and dicot intron-containing fragments inserted in the 5′ untranslated leader, between the CaMV 35S promoter and the uidA gene (coding for the ß-glucuronidase: GUS). Relative strengths of the intron-containing fragments were evaluated by comparing transient GUS expression after particle bombardment in embryogenic maize and bluegrass suspension cultures. Our results confirm a dramatic dependence on the presence of an intron for chimeric gene expression in both species. On average, the maize first intron of ubi1 provided the highest enhancement of gene expression in maize and bluegrass (71- and 26-fold enhancement, respectively). Half of the introns tested affected gene expression differently in bluegrass and maize. This suggests that the intron-mediated enhancement of gene expression generally obtained with maize may not be fully applicable to all monocots. We also report enhancement of gene expression (92-fold) in a monocot species by a dicot intron (chsA intron).
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Bruce, W.B. and Quail, P.H. (1990) Plant Cell 2, 1081–1089.
Callis, J., Fromm, M., and Walbot, V. (1987) Genes Devlop 1, 1183–1200.
Chibbar, R.N., Kartha, K.K., Leung, N., Qureshi, J. and Caswell, K. (1991) Genome 34, 453–460.
Chibbar, R.N., Kartha, K.K., Datla, R.S.S., Leung, N., Caswell, K., Mallard, C.S. and Steinhauer, L. (1993) Plant Cell Rep 12, 506–509.
Christensen, A.H., Sharrock, R.A. and Quail, P.H. (1992) Plant Mol Biol 18, 675–689.
Christou, P. (1994) Agro-Food-Industry Hi-Tech 17, 27.
Clancy, M., Vasil, V., Hannah, L.C. and Vasil, I.K. (1994) Plant Sci 98, 151–161.
Fennell, A. and Hauptmann, R. (1992) Plant Cell Rep 11, 567–570.
Finer, J.J. and McMullen, M.D. (1990) Plant Cell Rep 8, 586–590.
Finer, J.J., Vain, P., Jones, M.W. and McMullen, M.D. (1992) Plant Cell Rep 11, 232–328.
Gallo-Meagher, M. and Irvine, J.E. (1993) Plant Cell Rep 12, 666–670.
Gamborg, O.L., Miller, R.A. and Ojima, K. (1968) Exp Cell Res 50, 151–158.
Gasser, C.S., Simonsen, C.C., Schilling, J.W. and Schimke, R.T. (1982) Proc Natl Acad Sci 79, 6522–6526.
Goodall, G.J. and Filipowicz, W. (1989) Cell 59, 473–483.
Goodall, G.J. and Filipowicz, W. (1990) Plant Mol Biol 14, 727–733.
Goodall, G.J. and Filipowicz, W. (1991) EMBO J 10, 2635–2644.
Hamer, D.H. and Leder, P. (1979) Cell 18, 1299–1302.
Jefferson, R.A. (1987) Plant Mol Biol Rep 5, 387–405.
Jones, J.D.G., Shlumukov, L., Carland, F., English, J., Scofield, S.R., Bishop, G.J. and Harrison, K. (1992) Trans Res 1, 285–297.
Keith, B. and Chua, N-H. (1986) EMBO J 5, 2419–2425.
Kloesgen, R.B., Gierl, A., Schwarz-Sommer, Z. and Saedler, H. (1986) Mol Gen Genet 203, 237–244
Koes, R.E., Spelt C.E., van den Elven P.J.M. and Mol J.N.M. (1989) Gene 81, 245–257.
Kuhlemeir, C., Fluhr, R. and Chua, N-H. (1988) Mol Gen Genet 212, 405–411.
Last, D.I., Brettell, R.I.S., Chamberlain, D.A., Chaudhury, A.M., Larkin, P.J., Marsh, E.L., Peacock, W.J. and Dennis, E.S. (1991) Theor Appl Genet 81, 581–588.
Leon, P., Planckaert, F. and Walbot, V. (1991) Plant Physiol 95, 968–972.
Lyznik, L.A., McGee, J.D., Tung, P.Y., Bennetzen, J.L. and Hodges, T.K. (1991) Mol Gen Genetics 230, 209–218.
Luehrsen, K.R. and Walbot, V. (1991) Mol Gen Genet 225, 81–93.
Luehrsen, K.R. and Walbot, V. (1994) Plant Mol Biol 24, 449–463.
Maas, C., Laufs, J., Grant, S., Korfhage, C. and Werr, W. (1991) Plant Mol Biol 16, 199–207.
Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
McElroy, D., Zhang, W., Cao, J. and Wu, R. (1990) Plant Cell 2, 163–171.
McElroy, D. Blowers, A.D., Jenes, B. and Wu, R. (1991) Mol Gen Genet 231, 150–160.
Mascarenhas, D., Mettler, I.J., Pierce, D.A. and Lowe, H.W. (1990) Plant Mol Biol 15, 913–920.
Murashige, T. and Skoog, F. (1962) Physiol Plant 15, 474–497.
Morris, S.R., Meyer, S.E. and Callis, J. (1993) Plant Mol Biol 21, 895–906.
Paszkowski, J., Peterhans, A., Bilang, R. and Filipowicz, W. (1992) Plant Mol Biol 19, 825–836.
Ralston, E.J., English, J.J. and Dooner, H.K. (1988) Genetics 119, 185–197.
Rathus, C., Bower, R. and Birch, R.G. (1993) Plant Mol Biol 23, 613–618.
Schledzewski, K. and Mendel, R.R. (1994) Trans Res 3, 249–255.
Takumi, S., Otani, M. and Shimada, T. (1994) Plant Sci 103, 161–166.
Tanaka, A., Mita, S., Ohta, S., Kyozuka, J., Shimamoto, K. and Nakamura, K. (1990) Nucleic Acids Res 18, 6767–6770.
Taylor, M. G., Vasil, V. and Vasil, I. K. (1993) Plant Cell Rep 12, 491–495.
Vain, P., McMullen, M.D. and Finer, J.J. (1993a) Plant Cell Rep 12, 84–88.
Vain, P., Keen, N., Murillo, J., Rathus, C., Nemes, C. and Finer, J.J. (1993b) Plant Cell Tiss Org Cult 33, 237–246.
Vancanneyt, G., Schmidt, R., O'Connor-Sanchez, A., Willmitzer, L. and Rocha-Sosa, M. (1990) Mol Gen Genet 220, 245–250.
Vasil, V., Clancy, M., Ferl, R.J., Vasil, I.K. and Hannah, L.C. (1989) Plant Physiol 91, 1575–1579.
Vasil, V. (1994) Plant Mol Biol 25, 925–937.
Weising, K., Schell, J. and Kahl, G. (1988) Ann. Rev. Genet 22, 421–477.
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Vain, P., Finer, K.R., Engler, D.E. et al. Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.). Plant Cell Reports 15, 489–494 (1996). https://doi.org/10.1007/BF00232980
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DOI: https://doi.org/10.1007/BF00232980