Skip to main content
SpringerLink
Log in

Marker-assisted introgression of bacterial blight and blast resistance into DRR17B, an elite, fine-grain type maintainer line of rice

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

DRR17A is a stable wild-abortive cytoplasmic male sterile line with medium-slender grain type. DRR17A and its maintainer line DRR17B are highly susceptible to two of the major rice diseases, bacterial blight (BB) and blast. To improve DRR17B for resistance against BB and blast, we have introgressed a major dominant gene each conferring resistance against BB (Xa21) and blast (Pi54) into the maintainer line through marker-assisted backcross breeding using RP-Bio-Patho-2 (a near-isogenic line of Samba Mahsuri possessing Xa21 and Pi54) as the donor parent. PCR-based molecular markers tightly linked to Xa21 and Pi54 were used for foreground selection of the resistance plants at each backcross generation, while molecular markers tightly linked to the major fertility restorer genes, Rf3 and Rf4, were used for negative selection (i.e. selection of plants possessing non-fertility-restoring alleles at the two loci) at BC1 generation. After foreground selection for the target genes at each backcross generation, the ‘positive’ plants were screened with parental polymorphic markers for identifying backcross plants possessing maximum recovery of DRR17B genome. Marker-assisted backcrossing was continued till BC3 generation, and a single BC3F1 plant possessing the target genes with ~94 % recovery of recurrent parent genome was identified and selfed to generate BC3F2s. A total of six homozygous BC3F2 plants were identified and advanced. At BC3F5, six promising, stable, backcross-derived lines possessing high level of resistance against BB and blast, high yield, short plant stature, fine-grain type, have been identified; their maintenance ability and heterotic potential validated through test crosses and these lines are being converted to CMS lines through marker-assisted breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abenes MLP, Angeles ER, Khush GS, Huang N (1993) Selection of bacterial blight resistant rice plant in the F2 generation via their linkage to molecular markers. Rice Genet Newsl 10:120–123

    Google Scholar 

  • Ahmed MI, Siddiq EA (1998) Rice. In: Banga SS, Banga SK (eds) Hybrid cultivar development. Narosa Publishing House, New Delhi, pp 221–256

    Chapter  Google Scholar 

  • Balaji SP, Vemireddy LR, Srikanth B, Dharika N, Sambasiva Rao KRS, Hemanth Kishore V, Sundaram RM, Viraktamath BC, Subhakara Rao I, Ramesha MS, Neeraja CN (2012) Fine mapping of Rf3 and Rf4 fertility restorer loci of WA-CMS of rice (Oryza sativa L.) and validation of the developed marker system for identification of restorer lines. Euphytica 187:421–435

    Article  Google Scholar 

  • Ballini E, Morel JB, Droc G, Price A, Courtois B, Notteghem JL, Tharreau DA (2008) A genome-wide meta-analysis of rice blast resistance genes and quantitative trait loci provides new insights into partial and complete resistance. Mol Plant Microbe Interact 217:859–868

    Article  Google Scholar 

  • Basavaraj SH, Singh VK, Singh Singh A, Singh A, Anand D, Yadav S, Ellur RK, Singh D, KrishnanS Gopala, Nagarajan M, Mohapatra T, Prabhu KV, Singh AK (2010) Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid. Mol Breed 26:293–305

    Article  CAS  Google Scholar 

  • Bhasin H, Bhatia Dharminder, Raghuvanshi Saurabh, Jagjit S, Lore Gurpreet K, Sahi Baljit Kaur, Vikal Yogesh, Singh Kuldeep (2012) New PCR-based sequence-tagged site marker for bacterial blight resistance gene Xa38 of rice. Mol Breed 30:607–611

    Article  CAS  Google Scholar 

  • Cheema KK, Grewal NK, Vikal Y, Sharma R, Lore JS, Das A, Bhatia D, Mahajan R, Gupta V, Bharaj TS, Singh K (2008) A novel bacterial blight resistance gene from Oryza nivara mapped to 38 Kb region on chromosome 4 and transferred to Oryza sativa L. Genet Res 90:1–11

    Article  Google Scholar 

  • Chen SX, Lin H, Xu CG, Zhang Q (2000) Improvement of bacterial blight resistance of ‘Minghui 63’, an elite restorer line of hybrid rice by molecular marker-assisted selection. Crop Sci 40:239–244

    Article  Google Scholar 

  • Chen SC, Xu G, Lin XH, Zhang Q (2001) Improving bacterial blight resistance of 6078, an elite restorer line of hybrid rice by molecular marker-aided selection. Plant Breed 120:133–137

    Article  CAS  Google Scholar 

  • Collard BCY, Mackill DJ (2008) Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Phil Trans R Soc B 363:557–572

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Davierwala AP, Reddy APK, Lagu MD, Ranjekar PK, Gupta VS (2001) Marker assisted selection of bacterial blight resistance genes in rice. Biochem Genet 39:261–278

    Article  CAS  PubMed  Google Scholar 

  • DRR progress report vol. 2 (2008–2013) All India coordinated rice improvement programme (ICAR). Directorate of Rice Research, Rajendranagar, Hyderabad, India

  • Gopalakrishnan S, Sharma RK, Anand Rajkumar K, Joseph M, Singh VP, Singh AK, Bhat KV, Singh NK, Mohapatra T (2008) Integrating marker assisted background analysis with foreground selection for identification of superior bacterial blight resistant recombinants in Basmati rice. Plant Breed 127:131–139

    Article  CAS  Google Scholar 

  • Hari Y, Srinivasarao K, Viraktamath BC, Hariprasad AS, Laha GS, Ilyas Ahmed M, Natarajkumar P, Ramesha MS, Neeraja CN, Balachandran SM, Shobha Rani N, Balaji Suresh P, Sujatha K, Pandey M, Ashok Reddy G, Madhav MS, Sundaram RM (2011) Marker-assisted improvement of a stable restorer line, KMR-3Rand its derived hybrid KRH2 for bacterial blight resistance and grain quality. Plant Breed 130:608–616

    Article  CAS  Google Scholar 

  • Hari Y, Srinivasa Rao K, Viraktamath BC, Hariprasad AS, Laha GS, Ahmed M, Nataraj Kumar P, Sujatha K, Srinivasprasad MS, Rani NS, Balachandran SM, Kemparaju S, Mohan KM, Sama VSAK, Shaik H, Balachiranjeevi CH, Pranathi K, Reddy GA, Madhav MS, Sundaram RM (2013) Marker-assisted introgression of bacterial blight and blast resistance into IR 58025B, an elite maintainer line of rice. J Plant Breed 132:586–594

    Article  CAS  Google Scholar 

  • Hospital F, Moreau L, Lacoudre F, Charcosset A, Gallais A (1997) More on the efficiency of marker-assisted selection. Theor Appl Genet 95:1181–1189

    Article  Google Scholar 

  • Huang N, Angeles ER, Domingo J, Magpantay G, Singh S, Zhang Q, Kumaravadivel N, Bennett J, Khush GS (1997) Pyramiding of bacterial resistance genes in rice: marker aided selection using RFLP and PCR. Theor Appl Genet 95:313–320

    Article  CAS  Google Scholar 

  • Hulbert SH, Webb CA, Smith SM, Sun Q (2001) Resistance gene complexes: evolution and utilization. Annu Rev Phytopathol 39:285–312

    Article  CAS  PubMed  Google Scholar 

  • International Rice Research Institute (1996) Standard evaluation system for rice, 4th edn. International Rice Research Institute, Manila

    Google Scholar 

  • Joseph M, Gopalakrishnan S, Sharma RK (2004) Combining bacterial blight resistance and basmati quality characteristics by phenotypic and molecular marker-assisted selection in rice. Mol Breed 13:377–387

    Article  CAS  Google Scholar 

  • Kauffman HE, Reddy APK, Hsieh SPY, Merca SD (1973) An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep 57:537–541

    Google Scholar 

  • Khanna A, Sharma V, Ellur RK, Shikari AB, Gopala Krishnan S, Singh UD, Prakash G, Sharma TR, Rathour R, Variar M, Prashanthi SK, Nagarajan M, Vinod KK, Bhowmick PK, Singh NK, Prabhu KV, Singh BD, Singh AK (2015) Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice. Theor Appl Genet. doi:10.1007/s00122-015-2502-4

    PubMed  Google Scholar 

  • Khush GS, Jena KK (2009) Current status and future prospects for research on blast resistance in rice (Oryza sativa L.). In: Advances in genetics, genomics and control of rice blast disease. pp 1–10

  • Khush GS, Bacalango E, Ogawa T (1990) A new gene for resistance to bacterial blight from O. longistaminata. Rice Genet Newsl 7:121–122

    Google Scholar 

  • Koide Y, Kobayashi N, Xu D, Fukuta Y (2009) Resistance genes and DNA selection markers for blast disease in rice Oryza sativa L. Jpn Agric Res 43:255–280

    Article  CAS  Google Scholar 

  • Laha GS, Reddy CS, Krishnaveni D, Sundaram RM, Srinivas PM, Ram T, Muralidharan K, Viraktamath BC (2009) Bacterial Blight of Rice and its Management. In: DRR Technical Bulletin No. 41. Directorate of Rice Research (ICAR), Hyderabad, India, p 37

  • Leung HJ, Liu WB, Bustaman M, Sridhar R, Singh K, Redona E, Quang VD, Zheng K, Bernardo M, Wang G, Leach J, Choi IR, Cruz CV (2004) Sustainable disease resistance in rice: current and future strategies. In: New directions for a diverse planet. 4th International Crop Sci Cong Sep–Oct 2004, Brisbane, Australia.Published on CDROM. Web site www.cropscience.org.au

  • Liyong CZ, Jie-yun Shou-jiang, Xiao-deng Z, Kang-le Z, Shi-hua C (2003) Hybrid rice resistant to bacterial leaf blight developed by marker assisted selection. Rice Sci 11:68–70

    Google Scholar 

  • Madhan Mohan K (2011) Molecular characterization of pathogenic variability of Pyricularia grisea (Rice Blast fungus). Dissertation, Jawaharlal Nehru Technological University Hyderabad, Andhra Pradesh, India

  • Mew TW (1987) Current status and future prospects of research on bacterial blight of rice. Annu Rev Phytopathol 25:359–382

    Article  Google Scholar 

  • Narayanan NN, Baisakh N, Vera Cruz N, Gnananmanickam SS, Datta K, Datta SK (2002) Molecular breeding for the development of blast and bacterial blight resistance in rice cv. IR50. Crop Sci 42:2072–2079

    Article  CAS  Google Scholar 

  • Natrajkumar P, Sujatha K, Laha GS, Srinivasa Rao K, Mishra B, Viraktamath BC, Hari Y, Reddy CS, Balachandran SM, Ram T, Sheshu Madhav M, Shobha Rani N, Neeraja CN, Ashok Reddy G, Shaik H, Sundaram RM (2012) Identification and fine-mapping of Xa33, a novel gene for resistance to Xanthomonas oryzae pv. oryzae. Phytopathology 102:222–228

    Article  Google Scholar 

  • Pandey MK, Shobha Rani N, Sundaram RM, Laha GS, Madhav MS, Srinivasa Rao K, Injey Sudharshan, Yadla Hari, Varaprasad GS, Subba Rao LV, Kota Suneetha, Sivaranjani AKP, Viraktamath BC (2013) Improvement of two traditional Basmati rice varieties 3 for bacterial blight resistance and plant stature through 4 morphological and marker-assisted selection. Mol Breed 31:239–246

    Article  Google Scholar 

  • Perez ML, Redona DE, Mendioro SM, Verna Cruz MC, Leung H (2008) Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermo sensitive genetic male sterile rice (Oryza sativa L.) for the development of two line hybrids. Euphytica 164:627–636

    Article  CAS  Google Scholar 

  • Perumalsamy S, Bharani M, Sudha M, Nagarajan P, Arul L, Saraswathi R, Balasubramanian P, Ramalingam J (2010) Functional marker-assisted selection for bacterial leaf blight resistance genes in rice (Oryza sativa L.). Plant Breed 129:400–406

    CAS  Google Scholar 

  • Rai AK, Kumar SP, Gupta SK, Gautam N, Singh NK, Sharma TR (2011) Functional complementation of rice blast resistance gene Pikh (Pi54) conferring resistance to diverse strains of Magnaporthe oryzae. J Plant Bioche Biotechnol 20:55–65

    Article  CAS  Google Scholar 

  • Ramesha MS, Viraktamath BC, Ilyas Ahmed M (2010) RPHR-1005 (IC569494; INGR09085), a Paddy (Oryza sativa) germplasm with broad spectrum of fertility restoration and with good plant type, high rate of pollen production and good combining ability BPT5204 derivative with short slender grain, lower panicle position and prominent top leaves. Indian J Plant Genet Resour 23(3):327–354

    Google Scholar 

  • Ramkumar G, Srinivasarao K, Madhan Mohan K, Sudarshan I, Sivaranjani AKP, Gopalakrishna K, Neeraja CN, Balachandran SM, Sundaram RM, Prasad MS, Shobha Rani N, Rama Prasad AM, Viraktamath BC, Madhav MS (2010) Development and validation of functional marker targeting an In Del in the major rice blast disease resistance gene Pi54 (Pikh). Mol Breed 27:129–135

    Article  Google Scholar 

  • Rao KK, Lakshminarasu M, Jena KK (2002) DNA markers and marker-assisted breeding for durable resistance to bacterial blight disease in rice. Biotechnol Adv 20:33–47

    Article  CAS  PubMed  Google Scholar 

  • Ronald PC, Albano B, Tabien R, Abenes L, Wu K, Mc Couch S, Tanksley S (1992) Genetic and physical analysis of the rice bacterial blight resistance locus, Xa21. Mol Gen Genet 235:113–120

    Article  Google Scholar 

  • Sanchez AC, Brar DS, Huang N, Khush GS (2000) Sequence tagged site markers-assisted selection for three bacterial blight resistance genes in rice. Crop Sci 40:792–797

    Article  CAS  Google Scholar 

  • Shaik H, Yugander A, Balachiranjeevi CH, Pranathi K, Anila M, Mahadevaswamy HK, Kousik BVN, Dilip Kumar T, Ashok Reddy G, Bhaskar S, Abhilash Kumar V, Harika G, Rekha G, Laha GS, Viraktamath BC, Balachandran SM, Neeraja CN, Sheshu Madhav M, Mangrauthia SK, Bhadana VP, Sundaram RM (2014) Development of durable bacterial blight resistant lines of samba mahsuri possessing Xa33, Xa21, Xa13 & Xa5. Progr Res 9:1224–1227

    Google Scholar 

  • Shanti LM, Mohan Kumar Varma C, Premalatha P, Lalitha Devi G, Zher Usha, Freeman Wayne (2010) Understanding the bacterial blight pathogen combining pathotyping and molecular marker studies. Int J Plant Pathol 1(2):58–68

    Article  Google Scholar 

  • Sharma TR, Madhav MS, Singh BK, Shanker P, Jana TK, Dalal T, Pandit A, Singh A, Gaikwad K, Upreti HC, Singh NK (2005) High resolution mapping, cloning and molecular characterization of the Pik h gene of rice, which confers resistance to M. grisea. Mol Genet Genomics 274:569–578

    Article  CAS  PubMed  Google Scholar 

  • Sharma TR, Rai AK, Gupta SK, Singh NK (2010) Broad spectrum blast resistance gene Pikh cloned from the rice line Tetep designated as Pi54. J Plant Biochem Biotechnol 19:87–89

    Article  CAS  Google Scholar 

  • Sharma TR, Rai AK, Gupta SK, Vijayan J, Devanna BN, Ray S (2012) Rice blast management through host resistance: retrospect and prospects. Agric Res 1:37–52

    Article  Google Scholar 

  • Singh S, Sidhu JS, Huang N, Vikal Y, Li Z, Brar DS, Dhaliwal HS, Khush GS (2001) Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker- assisted selection into indica rice cultivar PR-106. Theor Appl Genet 102:1011–1015

    Article  CAS  Google Scholar 

  • Singh AK, Gopala Krishnan S, Singh VP, Prabhu KV, Mohapatra T, Singh NK, Sharma T, Nagarajan M, Vinod KK, Singh D, Singh UD, Chander S, Atwal SS, Seth R, Singh VK, Ellur RK, Singh A, Anand D, Khanna A, Yadav S, Goel N, Singh A, Shikari AB, Singh A, Marathi B (2011) Marker assisted selection: a paradigm shift in Basmati breeding. Indian J Genet Plant Breed 71:1–9

    Google Scholar 

  • Singh VK, Singh A, Singh SP, Ellur RK, Choudhary V, Singh D, Gopala Krishnan S, Nagarajan M, Vinod KK, Singh UD, Prashanthi SK, Agrawal PK, Bhatt JC, Mohapatra T, Prabhu KV, Sarkel S, Rathore R, Singh AK (2012) Incorporation of blast resistance into “PRR78”, an elite Basmati rice restorer line, through marker assisted backcross breeding. Field Crops Res 12:8–16

    Article  Google Scholar 

  • Song WY, Wang GL, Chen LL, Kim HS, Pi LY, Holsten T, Gardner J, Wang B, Zhai WX, Zhu LH, Fauquet C, Ronald PC (1995) A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Sci 270:1804–1806

    Article  CAS  Google Scholar 

  • Sonti RV (1998) Bacterial leaf blight of rice: new insights from molecular genetics. Curr Sci 74:206–212

    CAS  Google Scholar 

  • Sreewongchai T, Toojinda T, Thanintorn N, Kosawang C, Vanavichit A, Tharreau D, Sirithunya P (2010) Development of elite indica rice lines with wide spectrum of resistance to Thai blast isolates by pyramiding multiple resistance QTLs. Plant Breed 129:176–180

    Article  CAS  Google Scholar 

  • Srinivas Prasad M, Shesu Madhav M, Laha GS, Ladha Lakshmi D, Krishnaveni D, Mangrauthia SK, Balachandran SM, Sundaram RM, Arunakanthi B, Madhan Mohan K, Ratna Madhavi K, Kumar V, Virakatamath BC (2011) Rice blast disease and its management. Technical Bulletin No. 57. Directorate of Rice Research (ICAR), Hyderabad

  • Srinivasan B, Gnanamanickam S (2005) Identification of a new source of resistance in wild rice, Oryza rufipogon to bacterial blight of rice caused by Indian strains of Xanthomonas oryzae pv. Oryzae. Curr Sci 88:25

    Google Scholar 

  • Stoskopf NC, Tomes DT, Christie BR (1993) Plant breeding: theory and practice. West view Press Inc., Oxford, San Francisco

    Google Scholar 

  • Sujatha K, Natarajkumar P, Laha GS, Mishra B, Srinivasarao K, Viraktamath BC, Kirti PB, Hari Y, Balachandran SM, Rajendrakumar P, Ram T, Hajira SK, Madhav MS, Neeraja CN, Sundaram RM (2011) Inheritance of bacterial blight resistance in the rice cultivar Ajaya and high-resolution mapping of a major QTL associated with resistance. Genet Res Camb 93:397–408

    Article  CAS  PubMed  Google Scholar 

  • Sundaram RM, Vishnupriya MR, Biradar SK, Laha GS, Reddy GA, Rani NS, Sarma NP, Sonti RV (2008) Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica 160:411–422

    Article  Google Scholar 

  • Sundaram RM, Vishnupriya MR, Laha GS, Shobha Rani N, SrinivasRao P, Balachandaran SM, Ashok Reddy G, Sarma NP, Shonti RV (2009) Introduction of bacterial blight resistance into Triguna, a high yielding, mid-early duration rice variety. Biotechnol J 4:400–407

    Article  CAS  PubMed  Google Scholar 

  • Sundaram RM, Chatterjee S, Oliva R, Laha GS, Leach JE, Sonti RV, Cruz CV (2014) Update on bacterial blight of rice: fourth international conference on bacterial blight rice 7: 12

  • Virmani SS (1996) Hybrid rice. Adv Agron 57:377–462

    Article  Google Scholar 

  • Virmani SS, Kumar I (2004) Development and use of hybrid rice technology to increase rice productivity in the tropics. Int Rice Res Notes 29:10–20

    Google Scholar 

  • Wang GL, Song WY, Ruan DL, Sideris S, Ronald PC (1996) The cloned Xa21 confers resistance to multiple Xanthomonas oryzae pv. Oryzae isolates in transgenic plants. Mol Plant Microbiol Interact 9:850–855

    Article  CAS  Google Scholar 

  • Yoshimura S, Yoshimura A, Iwata N, Mc Couch SR, Abenes MN, Baraoidan MR, Mew TW, Nelson RJ (1995) Tagging and combining bacterial blight resistance genes using RAPD and RFLP markers. Mol Breed 1:375–387

    Article  CAS  Google Scholar 

  • Zhan XD, Zhou HP, Chai RY, Zhuang JY, Cheng SH, Li CY (2012) Breeding of R8012, a Rice restorer line resistant to blast and bacterial blight through marker-assisted selection. Rice Sci 19(1):29–35

    Article  CAS  Google Scholar 

  • Zhang G, Angeles ER, Abenes MLP, Khush GS, Huang N (1996) RAPD and RFLP mapping of the bacterial blight resistance gene xa13 in rice. Theor Appl Genet 93:65–70

    Article  CAS  PubMed  Google Scholar 

  • Zheng K, Subudhi PK, Domingo J, Maopanty G, Huang N (1995) Rapid DNA isolation for marker assisted selection in rice breeding. Rice Genet Newsl 12:255–258

    Google Scholar 

  • Zhou YL, Uzokwe VNE, Zhang CH, Cheng LR, Wang L, Chen K, Gao X, Sun Q, ChenY ZhuJJ, Zhang LH, Ali Q, Xu JL, Li ZK (2011) Improvement of bacterial blight resistance of hybrid rice in China using the Xa23 gene derived from wild rice (Oryza rufipogon). Field Crop Res 30:637–644

    CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to acknowledge the funding support provided by the Department of Biotechnology (DBT), Government of India, for execution of the research study through the Grant # BT/PR11705/AGR/02/646/2008. The authors also thank Project Director, ICAR-Indian Institute of Rice Research, for providing all the necessary facilities.

Author information

Author notes

    Authors and Affiliations

    1. Indian Institute of Rice Research, Rajendranagar, Hyderabad, 500030, India

      CH Balachiranjeevi, Naik S. Bhaskar, V. Abhilash, S. Akanksha, B. C. Viraktamath, M. S. Madhav, A. S. Hariprasad, G. S. Laha, M. S. Prasad, S. M. Balachandran, C. N. Neeraja, M. Satendra Kumar, P. Senguttuvel, K. B. Kemparaju, V. P. Bhadana, T. Ram, G. Harika, H. K. Mahadeva Swamy, S. K. Hajira, A. Yugander, K. Pranathi, M. Anila, G. Rekha, M. B. V. N. Kousik, T. Dilip Kumar, R. K. Swapnil & R. M. Sundaram

    2. Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, 500085, India

      Archana Giri

    Authors
    1. CH Balachiranjeevi
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Naik S. Bhaskar
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. V. Abhilash
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. S. Akanksha
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. B. C. Viraktamath
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. M. S. Madhav
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. A. S. Hariprasad
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. G. S. Laha
      View author publications

      You can also search for this author in PubMed Google Scholar

    9. M. S. Prasad
      View author publications

      You can also search for this author in PubMed Google Scholar

    10. S. M. Balachandran
      View author publications

      You can also search for this author in PubMed Google Scholar

    11. C. N. Neeraja
      View author publications

      You can also search for this author in PubMed Google Scholar

    12. M. Satendra Kumar
      View author publications

      You can also search for this author in PubMed Google Scholar

    13. P. Senguttuvel
      View author publications

      You can also search for this author in PubMed Google Scholar

    14. K. B. Kemparaju
      View author publications

      You can also search for this author in PubMed Google Scholar

    15. V. P. Bhadana
      View author publications

      You can also search for this author in PubMed Google Scholar

    16. T. Ram
      View author publications

      You can also search for this author in PubMed Google Scholar

    17. G. Harika
      View author publications

      You can also search for this author in PubMed Google Scholar

    18. H. K. Mahadeva Swamy
      View author publications

      You can also search for this author in PubMed Google Scholar

    19. S. K. Hajira
      View author publications

      You can also search for this author in PubMed Google Scholar

    20. A. Yugander
      View author publications

      You can also search for this author in PubMed Google Scholar

    21. K. Pranathi
      View author publications

      You can also search for this author in PubMed Google Scholar

    22. M. Anila
      View author publications

      You can also search for this author in PubMed Google Scholar

    23. G. Rekha
      View author publications

      You can also search for this author in PubMed Google Scholar

    24. M. B. V. N. Kousik
      View author publications

      You can also search for this author in PubMed Google Scholar

    25. T. Dilip Kumar
      View author publications

      You can also search for this author in PubMed Google Scholar

    26. R. K. Swapnil
      View author publications

      You can also search for this author in PubMed Google Scholar

    27. Archana Giri
      View author publications

      You can also search for this author in PubMed Google Scholar

    28. R. M. Sundaram
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to R. M. Sundaram.

    Additional information

    CH Balachiranjeevi, S. Bhaskar Naik, V. Abhilash and R. M. Sundaram have contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Supplementary material 1 (PPT 349 kb)

    Supplementary material 2 (DOCX 13 kb)

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Balachiranjeevi, C., Bhaskar, N.S., Abhilash, V. et al. Marker-assisted introgression of bacterial blight and blast resistance into DRR17B, an elite, fine-grain type maintainer line of rice. Mol Breeding 35, 151 (2015). https://doi.org/10.1007/s11032-015-0348-8

    Download citation

    • Received:

    • Accepted:

    • Published:

    • DOI: https://doi.org/10.1007/s11032-015-0348-8

    Keywords

    Search

    Navigation