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Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid

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Abstract

Pusa RH10, the widely cultivated superfine grain aromatic rice hybrid, and its parental lines Pusa6B and PRR78 are susceptible to bacterial blight (BB) disease caused by Xanthomonas oryzae pv. oryzae. Pusa1460, a Basmati rice variety, was utilized as the donor for introgressing BB resistance genes xa13 and Xa21 into Pusa6B and PRR78 using a marker-assisted backcross breeding program. The markers RG136 and pTA248 linked to BB resistance genes xa13 and Xa21, respectively, were used for foreground selection. Seventy-four STMS markers polymorphic between Pusa6B and Pusa1460, and 54 STMS markers polymorphic between PRR78 and Pusa1460, were utilized for background selection to recover the recurrent parent genome ranging from 85.14 to 97.30% and 87.04 to 92.81% in the 10 best BC2F5 families of Pusa6B and PRR78, respectively. RM6100, an STMS marker linked to fertility restorer gene (Rf), was used for marker-assisted selection of Rf gene in an improved version of PRR78. The extent of donor segments in the improved version of Pusa6B was estimated to be <0.97 and <2.15 Mb in the genomic regions flanking xa13 and Xa21, respectively, whereas in improved PRR78, it was estimated to be <2.07 and <3.45 Mb in the corresponding genomic regions. Improved lines of Pusa6B and PRR78 showed yield advantages of up to 8.24 and 5.23%, respectively. The performance of the BB-resistant version of Pusa RH10 produced by intercrossing the improved parental lines was on a par with or superior to the original Pusa RH10.

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Abbreviations

CAPS:

Cleaved amplified polymorphic sequences

MABB:

Marker-assisted backcross breeding

MAS:

Marker-assisted selection

RPG:

Recurrent parent genome

STMS:

Sequenced tagged microsatellite sites

Xoo :

Xanthomonas oryzae pv. oryzae

References

  • Basavaraj SH, Singh VK, Singh A, Singh D, Nagarajan M, Mohapatra T, Prabhu KV, Singh AK (2009) Marker aided improvement of Pusa6B, the maintainer parent of hybrid Pusa RH10, for resistance to bacterial blight. Indian J Genet Plant Breed 69(1):10–16

    CAS  Google Scholar 

  • Cheema KK, Grewal NK, Vikal Y, Das A, Sharma R, Lore JS, Bhatia D, Mahajan R, Gupta V, Singh K (2008) A novel bacterial blight resistance gene from Oryza nivara, mapped to 176 kb region and transferred to Oryza sativa L. Genet Res 90:397–407

    Article  CAS  PubMed  Google Scholar 

  • Chen S, Lin XH, Xu CG, Zhang Q (2000) Improvement of bacterial blight of ‘Minghui-63’ an elite restorer line of hybrid rice by molecular marker assisted selection. Crop Sci 40:239–244

    Article  Google Scholar 

  • Chen S, Xu CG, Lin XH, Zhang Q (2001) Improving bacterial blight resistance of ‘6078’, an elite restorer line of hybrid rice, by molecular marker-aided selection. Plant Breed 120:133–137

    Article  CAS  Google Scholar 

  • Chu Z, Fu B, Yang H, Xu C, Li Z, Sanchez A, Park YJ, Bennetzen JL, Zhang Q, Wang S (2006) Targeting xa13, a recessive gene for bacterial blight resistance in rice. Theor Appl Genet 112(3):455–461

    Article  CAS  PubMed  Google Scholar 

  • Gnanamanickam SS, Brindha Priyadarshini PV, Naryanan NN, Vasudevan P, Kavitha S (1999) An overview of bacterial blight disease of rice and strategies for its management. Curr Sci 77:1435–1444

    Google Scholar 

  • Gopalakrishnan S, Sharma RK, Anand Rajkumar K, Joseph M, Singh VP, Singh AK, Bhat KV, Singh NK, Mohapatra T (2008) Integrating marker assisted background analysis with foreground selection for identification of superior bacterial blight resistant recombinants in Basmati rice. Plant Breed 127:131–139

    Article  CAS  Google Scholar 

  • Hospital F, Chavalet C, Mubsant P (1992) Using markers in gene introgression breeding programmes. Genetics 132:1119–1210

    Google Scholar 

  • Huang N, Angeles ER, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bennett J, Khush GS (1997) Pyramiding of bacterial blight resistance genes in rice: marker assisted selection using RFLP and PCR. Theor Appl Genet 95:313–320

    Article  CAS  Google Scholar 

  • Joseph M, Gopalakrishnan S, Sharma RK, Singh VP, Singh AK, Singh NK, Mohapatra T (2004) Combining bacterial blight resistance and basmati quality characteristics by phenotypic and molecular marker assisted selection in rice. Mol Breed 13:377–387

    Article  CAS  Google Scholar 

  • Kauffman HE, Reddy APK, Hsieh SPY, Merca SD (1973) An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep 57:537–541

    Google Scholar 

  • Khush GS, Mackill DJ, Sidhu GS (1989) Breeding rice for resistance to bacterial blight. In: RI IR (ed) Bacterial blight of rice. IRRI, Manila, pp 207–217

    Google Scholar 

  • Little RR, Hilder GB, Dawson EH (1958) Differential effect of dilute alkali on 25 varieties of milled white rice. Cereal Chem 35:111–126

    CAS  Google Scholar 

  • Luo YC, Wang SH, Li CQ, Wu S, Wang DZ, Du SY (2005) Improvement of bacterial blight resistance by molecular marker-assisted selection in a wide compatibility restorer line of hybrid rice. Chin J Rice Sci 19:36–40

    CAS  Google Scholar 

  • McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom RG, De Clerk G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res 9:199–207

    Article  CAS  PubMed  Google Scholar 

  • Narayanan NN, Baisakh N, Oliva NP, VeraCruz CV, Gnanamanickam SS, Datta K, Datta SK (2004) Molecular breeding: marker-assisted selection combined with biolistic transformation for blast and bacterial blight resistance in Indica rice (cv. CO39). Mol Breed 14:61–67

    Article  CAS  Google Scholar 

  • Panaud O, Chen X, McCouch SR (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet 252:597–607

    CAS  PubMed  Google Scholar 

  • Perez ML, Redona DE, Mendioro SM, Verna Cruz MC, Leung H (2008) Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice (Oryza sativa L.) for the development of two-line hybrids. Euphytica 164:627–636

    Article  CAS  Google Scholar 

  • Prabhu KV, Somers DJ, Rakow G, Gugel RK (1998) Molecular markers linked to white rust resistance in mustard Brassica juncea. Theor Appl Genet 97:865–870

    Article  CAS  Google Scholar 

  • Prakash P (2003) Molecular mapping of fertility restorer gene(s) and validation of Rf-gene linked markers in rice. MSc Dissertation, Indian Agricultural Research Institute

  • Reddy APK (1989) Bacterial blight crop loss assessment and disease management. In: Bacterial blight of rice. IRRI, Los Banos, pp 79–88

  • Sanchez AC, Brar DS, Huang N, Li Z, Khush GS (2000) Sequence tagged site marker-assisted selection for three bacterial blight resistance genes in rice. Crop Sci 40:792–797

    CAS  Google Scholar 

  • Siddiq EA, Singh VP, Zaman FU, Sadananda AR, Abraham MJ, Hari Prasad AS, Mahendru A, Natrajan US, Nagarajan M, Atwal SS, Sinha SN, Chopra NK, Seth R, Mohapatra T, Prabhu KV, Singh AK (2009) Development of high yielding basmati quality rice varieties: a success story. Ind Farm 59:13–17

    Google Scholar 

  • Singh S, Sidhu JS, Huang N, Vikal Y, Li Z, Brar DS, Dhaliwal HS, Khush GS (2001) Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into Indica cultivar PR 106. Theor Appl Genet 102:1011–1015

    Article  CAS  Google Scholar 

  • Sood BC, Siddiq EA (1978) A rapid technique for scent determinations in rice. Indian J Genet Plant Breed 38:2268–2271

    Google Scholar 

  • Stam P, Zeven AC (1981) The theoretical proportion of the donor genome in near isogenic lines of self fertilizers bred by backcrossing. Euphytica 30:227–238

    Article  Google Scholar 

  • Sundaram RM, Vishnupriya RM, Biradar SK, Laha GS, Ashok Reddy G, Shobha Rani N, Sarma NP, Sonti RV (2008) Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica 160:411–422

    Article  Google Scholar 

  • Sundaram RM, Vishnupriya MR, Laha GS, Shobha Rani N, Srinivas Rao P, Balachandaran SM, Asho Reddy G, Sarma NP, Shonti RV (2009) Introduction of bacterial blight resistance into Triguna, a high yielding, mid-early duration rice variety. Biotechnol J 4:400–407

    Article  CAS  PubMed  Google Scholar 

  • Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Biotechnology 7:257–264

    Article  CAS  Google Scholar 

  • Temnykh S, Park WD, Ayres N, Cartinhour S, Hauck N, Lipovich L, Cho YG, Ishii T, McCouch SR (2000) Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor Appl Genet 100:697–712

    Article  CAS  Google Scholar 

  • Temnykh S, DeClerck G, Lukashova A, Lipovich L, Cartinhour S, McCouch SR (2001) Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential. Genome Res 11:1441–1452

    Article  CAS  PubMed  Google Scholar 

  • Van Berloo R (1999) GGT: software for display of graphical genotypes. J Hered 90:328–329

    Article  Google Scholar 

  • Virmani SS (1996) Hybrid rice. Adv Agron 57:377–462

    Article  Google Scholar 

  • Virmani SS, Edwards IB (1983) Current status and future prospects for breeding hybrid rice and wheat. Adv Agron 36:145–214

    Article  Google Scholar 

  • Wang L, Zhang Z, Wei L, Zhang D, Teng F, Tao Y, Zheng Y (2009) The residual background genome from a donor within an improved line selected by marker-assisted selection: impact on phenotype and combining ability. Plant Breed 128:429–435

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was carried out under “Network Project on Gene Pyramiding in Crops” funded by the Indian Council of Agricultural Research, New Delhi, India. The first author acknowledges the Council of Scientific and Industrial Research for awarding the Junior Research Fellowship for PhD programme at the Indian Agricultural Research Institute, part of which is reported in this study.

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Authors and Affiliations

  1. Rice Section, Division of Genetics, Indian Agricultural Research Institute, New Delhi, 110012, India

    S. H. Basavaraj, Vikas K. Singh, Atul Singh, Ashutosh Singh, Anita Singh, Deepti Anand, Sheel Yadav, Ranjith K. Ellur, Devinder Singh, S. Gopala Krishnan, K. V. Prabhu & Ashok K. Singh

  2. Rice Breeding and Genetics Research Centre, IARI, Aduthurai, Tamil Nadu, India

    M. Nagarajan

  3. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India

    T. Mohapatra

Authors
  1. S. H. Basavaraj
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  2. Vikas K. Singh
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  3. Atul Singh
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  4. Ashutosh Singh
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  5. Anita Singh
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  6. Deepti Anand
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  7. Sheel Yadav
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  8. Ranjith K. Ellur
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  9. Devinder Singh
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  10. S. Gopala Krishnan
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  11. M. Nagarajan
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  12. T. Mohapatra
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  13. K. V. Prabhu
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  14. Ashok K. Singh
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Correspondence to Ashok K. Singh.

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Basavaraj, S.H., Singh, V.K., Singh, A. et al. Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid. Mol Breeding 26, 293–305 (2010). https://doi.org/10.1007/s11032-010-9407-3

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  • DOI: https://doi.org/10.1007/s11032-010-9407-3

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