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Marker-assisted introgression of the major bacterial blight resistance gene, Xa21 and blast resistance gene, Pi54 into RPHR-1005, the restorer line of the popular rice hybrid, DRRH3

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Abstract

The elite Indian rice hybrid, DRRH3 is highly susceptible to two major diseases, bacterial blight (BB) and blast, which limit its productivity significantly. In the present study, we have introgressed two major genes, viz., Xa21 and Pi54 conferring resistance against BB and blast, respectively into RPHR-1005, the male parent of DRRH3 through marker-assisted backcross breeding (MABB) and analyzed the backcross derived plants for their resistance against BB and blast. RPBio Patho-2 was used as a donor for both the resistance genes. Gene-specific markers were used for the foreground selection of Xa21 and Pi54 at each stage of backcrossing and markers specific for the major fertility restorer genes, Rf3 and Rf4 were used only at BC1F1 generation for foreground selection. Background selection was done using 62 polymorphic SSR markers and marker-assisted backcrossing was continued till BC3 generation. At BC3F4, through intensive phenotype-based selections 15 promising lines (ABLs) possessing high level of resistance against BB and blast, high yield, fine-grain type, complete fertility restoration along with better panicle exsertion and taller plant type as compared to RPHR-1005 were identified and test crossed with APMS 6 A, the female parent of DRRH3. The newly derived hybrids (i.e. improved versions of DRRH3) were observed to possess high level of resistance against BB and blast along with medium-slender grain type and yield level better than or equivalent to that of DRRH3. Our study exemplifies the utility of MABB for targeted improvement of multiple traits in hybrid rice.

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References

  • Ahmed MI, Siddiq EA (1998) Rice. In: Banga SS, Banga SK (eds) Hybrid Cultivar Development. Narosa Publishing House, New Delhi, pp. 221–256

    Chapter  Google Scholar 

  • Balachiranjeevi CH, Naik SB, Abhilash V, Akanksha S, Viraktamath BC, Madhav MS, Hariprasad AS, Laha GS, Prasad MS, Balachandran SM, Neeraja CN, Satendra Kumar M, Senguttuvel P, Kemparaju KB, Bhadana VP, Ram T, Harika G, Mahadeva Swamy HK, Hajira SK, Yugander A, Pranathi K, Anila M, Rekha G, Kousik MBVN, Dilip Kumar T, Swapnil RK, Giri A, Sundaram RM (2015) Marker-assisted introgression of bacterial blight and blast resistance into DRR17B, an elite, fine-grain type maintainer line of rice. Mol Breed 35:151

    Article  Google Scholar 

  • Balaji Suresh P, Srikanth B, Hemanth Kishore V, Subhakara Rao I, Vemireddy L, Dharika N, Sundaram R, Ramesha M, Sambasiva Rao K, Viraktamath B, Neeraja C (2012) Fine mapping of Rf3 and Rf4 fertility restorer loci of WA-CMS of rice (Oryzasativa L.) and validation of the developed marker system for identification of restorer line. Euphytica 187(3):421–435

    Article  CAS  Google Scholar 

  • Basavaraj SH, Singh VK, Singh A, Singh A, Singh A, Anand D, Yadav S, Gopalakrishnan S, Nagarajan M, Mohapatra T, KV P, AK S (2010) Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a super fine grain aromatic rice hybrid. Mol Breed 26:293–305

    Article  CAS  Google Scholar 

  • Bhasin H, Bhatia D, Raghuvanshi S, Lore JS, Sahi GK, Kaur B, Vikal Y, Singh K (2012) New PCR-based sequence-tagged site marker for bacterial blight resistance gene Xa38 of rice. Mol Breed 30:607–611

    Article  CAS  Google Scholar 

  • Cheema KK, Grewal NK, Vikal Y, Sharma R, Lore LS, Das A, Bhatia D, Mahajan R, Gupta V, Bharaj TS, Singh K (2008) A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4 and transferred to Oryza sativa L. Genet Res 90:1–11

    Article  Google Scholar 

  • Gu K, Yang B, Tian D, Wu L, Wang D, Sreekala C, Yang F, Chu Z, Wang G, White FF, Yin Z (2005) R gene expression induced by a type-II effector triggers disease resistance in rice. Nature 435:1122–1125

    Article  CAS  PubMed  Google Scholar 

  • Hari Y, Srinivasarao K, Viraktamath BC, Hariprasad AS, Laha GS, IlyasAhmed M, Natarajkumar P, Ramesha MS, Neeraja CN, Balachandran SM, ShobhaRani N, BalajiSuresh P, Sujatha K, Pandey M, AshokReddy G, Madhav MS, Sundaram RM (2011) Marker-assisted improvement of a stable restorer line, KMR-3R and its derived hybrid KRH2 for bacterial blight resistance and grain -quality. Plant Breed 130(60):608–616

    Article  CAS  Google Scholar 

  • Hari Y, Srinivasarao K, Viraktamath BC, AS HP, Laha GS, Ahmed IM, Natrajkumar P, Sujatha K, Srinivasa Prasad M, Pandey M, MS R, CN N, SM B, NS R, Balachandra K, Madan Mohan K, Venkata S, ArunSama K, Shaik H, Balachiranjeevi C, Pranathi Ashok R, Seshumadhav M, RM S (2013) Marker assisted introgression of bacterial blight and blast resistance into IR58025B, an elite maintainer line of rice. Plant Breed 132(6):586–594

    Article  CAS  Google Scholar 

  • ICAR (Indian Council of Agricultural Report), 2015, Progress Report-2014, All India Co-ordinated Rice Improvement Project (AICRIP), Indian Institute of Rice Research, Hyderabad, T. S.

  • IRRI 1996, Standard Evaluation System for rice, IRRI. INGER, 4th Edition, Pd-5.

  • Kauffman HE, Reddy APK, Hsieh SPY, Merca SD (1973) An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep 56:537–540

    Google Scholar 

  • Khan MA, Naeem M, Iqbal M (2014) Breeding approaches for bacterial leaf blight resistance in rice (Oryza sativa L.), current status and future directions. Eur J Plant Pathol 139:27–37

    Article  CAS  Google Scholar 

  • Khush GS, Jena KK (2009) Current status and future prospects for research on blast resistance in rice (Oryza sativa L.). In: Wang GL, Valent B (eds) Advances in genetics, genomics and control of rice blast disease. Springer, Dordrencht, pp. 1–10

    Chapter  Google Scholar 

  • Khush GS, MacKill DJ, Sidhu GS (1989) Breeding rice for resistance to bacterial blight. Proceedings of International Workshop on Bact. Blight Rice. IRRI, Manila, Philippines, pp. 207–217

    Google Scholar 

  • Kim SM, Suh JP, Qin Y, HNoh T, Reinke RF, Jena KK (2015) Identification and fine-mapping of a new resistance gene, Xa40, conferring resistance to bacterial blight races in rice (Oryza sativa L.). Theor Appl Genet doi: 10.1007/s00122–015-2557-2

  • Laha GS, Reddy CS, Krishnaveni D, Sundaram RM, Srinivas PM, Ram T, Muralidharan K, Viraktamath BC (2009) Bacterial Blight of Rice and its Management. Page37 in. DRR Technical Bulletin. No. Directorate of Rice Research (ICAR), Hyderabad

  • Lalitha DG, Pranitha ,K, Vinay ,S, Lalitha ,SM (2013) Improvement of resistance to bacterial blight through marker assisted backcross breeding and field validation in rice (Oryzasativa). Res J Bio 1:52–66

    Google Scholar 

  • Madhan Mohan K (2011) PhD thesis entitled Molecular characterization of pathogenic variability of Pyricularia grisea (Rice blast fungus) submitted to Faculty of Biotechnology. Jawaharlal Nehru Technological University, Hyderabad, Andhrapradesh, India

  • Mew TW (1987) Current status and future prospects of research on bacterial blight of rice. Annu Rev Phytopathol 25:359–382

    Article  Google Scholar 

  • Mew TW, Vera Cruz CM, Medalla ES (1992) Changes in race frequency of Xanthomonasoryzae. pv. oryzae in response to rice cultivars planted in the Philippines. Plant Dis 76:1029–1032

    Article  Google Scholar 

  • Natrajkumar P, Sujatha K, Laha GS, Srinivasarao K, Mishra B, Viraktamath BC, Hari Y, Reddy CS, Balachandran SM, Ram T, Sheshumadhav M, Shobharani N, Neeraja CN, Ashokreddy G, Shaik H, Sundaram RM (2012) Identification and fine-mapping of Xa33, a novel gene for resistance to Xanthomonas oryzae pv. oryzae. Phyto Path 102:222–228

    Article  Google Scholar 

  • Normile D (1999) Crossing rice strains to keep Asia’s rice bowl brimming. Science 283-313

  • Prasad M, Shesu Madhav M, Laha GS, Ladha Lakshmi D, Krishnaveni D, Mangrauthia SK, Balachandran SM, Sundaram RM, Arunakanthi B, Madhan Mohan K, Ratna Madhavi K, Kumar V, Virakatamath BC (2011) Rice Blast Disease and its Management. Technical Bulletin No. 57. Directorate of Rice Research (ICAR), Hyderabad, 52.

  • Ramesha MS, Virakatamath BC, Ilyas Ahmed M (2010) RPHR-1005 (IC569494; INGR09085), a Paddy (Oryza sativa) Germplasm with broad spectrum of fertility restoration and with good plant type, high rate of pollen production and good combining ability. BPT5204 derivative with short slender grain, lower panicle position and prominent top leaves. Indian J Plant Genet Resour 23(3):327–354

    Google Scholar 

  • Ramkumar G, Srinivasa Rao K, Madhan Mohan K, Sudarshan I, Sivaranjani AKP, Gopala Krishna K, Neeraja CN, Balachandran SM, Sundaram RM, Prasad MS, Shobha Rani N, Ram Prasad AM, Virakmath BC, Madhav MS (2011) Development and validation of functional marker targeting an In Del in the major rice blast disease resistance gene Pi54 (Pik h). Mol Breed 27:129–135

    Article  Google Scholar 

  • Ronald PC, Albano B, Tabien R, Wu Abenes L, Mc Couchand S, Tanksley S (1992) Genetic and physical analysis of the rice bacterial blight resistance locus, Xa21. Mol Gen Genet 235:113–120

    Article  Google Scholar 

  • Sharma TR, Madhav MS, Singh BK, Shanker P, Jana TK, Dalal V, Pandit A, Singh A, Gaikwad K, Upretiand HC, Singh NK (2005) High resolution mapping, cloning and molecular characterization of the Pik h gene of rice, which confers resistance to M. grisea. Mol Gene & Geno 274:569–578

    Article  CAS  Google Scholar 

  • Sharma TR, Rai AK, Guptaand GK, Singh NK (2010) Broad spectrum blast resistance gene Pik h cloned from the rice line tetep designated as Pi54. J. Plant Biochem Biotechnol 19:87–89

    Article  CAS  Google Scholar 

  • Sharma TR, Rai AK, Gupta SK, Vijayan J, Devannaand BN, Ray S (2012) Rice blast management through host-plant resistance: retrospect and Prospects. Agric Res 1(1):37–52

    Article  Google Scholar 

  • Singh VK, Singh A, Singh SP, Ellur RK, Choudhary V, Sarkhel S, Singh D, Gopala Krishnan S, Nagarajan M, Vinod KK, Singh UD, Rathore R, Prasanthi SK, Agrawal PK, Bhatt JC, Mohapatra T, Prabhu KV, Singh AK (2012) Incorporation of blast resistance into “PRR78”, an elite Basmati rice restorer line, through marker assisted backcross breeding. Field Cro Rese 128:8–16

    Article  Google Scholar 

  • Singh R, Sunder S, Dodan DS (2013) Estimation of losses in grain yield due to bacterial blight and neck blast of rice in Haryana. Indian Phytopath 66(3):249–251

    Google Scholar 

  • Spielman DJ, Deepthi EK, Patrick SW (2013) The prospects for hybrid rice in India. Food Sec. doi:10.1007/s12571-013-0291-7

    Google Scholar 

  • Sun X, Yang Z, Wang S, Zhang Q (2003) Identification of a 47 kb DNA fragment containing Xa4, a locus for bacterial blight resistance in rice. Theor Appl Genet 106:683–687

    Article  CAS  PubMed  Google Scholar 

  • Sundaram RM, Vishnupriya MR, Biradar SK, Laha GS, Reddy AG, Rani NS, Sharma NP, Sonti RV (2008) Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica 160:411–422

    Article  Google Scholar 

  • Sundaram RM, Priya MRV, Laha GS, Shobha Rani N, Srinivasa Rao P, Balachandran SM, Ashok Reddy G, Sarma NP, Sonti RV (2009) Introduction of bacterial blight resistance into Triguna, a high yielding, mid-early duration rice variety by molecular marker assisted breeding. Biotechnol J 4:400–407

    Article  CAS  PubMed  Google Scholar 

  • Van Berloo R (1999) GGT Software for display of graphical genotypes. J Heridity 90:328–329

    Article  Google Scholar 

  • Viraktamath BC, Hari Prasad AS, Ramesha MS, Ilyas Ahmed M (2010) Hybrid Rice in India, Technical Bulletin No 47, Directorate of Rice Research (ICAR), Rajendranagar, Hyderabad-500 030. A. P, India, p. 44

    Google Scholar 

  • Yang D, Sanchez A, Khush GS, Zhu Y, Huang N (1998) Construction of a BAC contig containing the xa5 locus in rice. Theor Appl Genet 97:1120–1124

    Article  CAS  Google Scholar 

  • Yuan LP (1994) Increasing yield potential in rice by exploitation of heterosis. In: Virmani SS (ed) Hybrid Rice Technology: New Developments and Future Prospects. International Rice Research Institute, Los Banos, Philippines, pp. 1–6

    Google Scholar 

  • Zheng K, Huang N, Bennett J, Khush GS (1995) PCR-based marker assisted selection in rice breeding. IRRI Discussion Paper Series No.12. International Rice Research Institute, Manila, Philippines

Download references

Acknowledgments

The authors would like to acknowledge the funding support provided by the Department of Biotechnology (DBT), Government of India for execution of the research study through the Grant # BT/PR11705/AGR/02/646/2008. The authors also thank Project Director, ICAR-IIRR for providing all the necessary facilities.

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Authors and Affiliations

  1. Indian Institute of Rice Research, Rajendranagar, Hyderabad, 500030, India

    V. Abhilash Kumar, C. H. Balachiranjeevi, S. Bhaskar Naik, R. Rambabu, G. Rekha, K. R. Madhavi, G. Harika, S. Vijay, K. Pranathi, S. K. Hajira, A. Srivastava, H. K. Mahadevaswamy, M. Anila, A. Yugander, J. Aruna, A. S. Hari Prasad, M. S Madhav, G. S. Laha, B. C. Viraktamath, S. M. Balachandran, P. Senguttuvel, B. Kemparaju, V. Ravindra Babu, R. M. Sundaram & M. S. Prasad

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  1. V. Abhilash Kumar
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  2. C. H. Balachiranjeevi
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  3. S. Bhaskar Naik
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  18. G. S. Laha
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  19. B. C. Viraktamath
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Corresponding authors

Correspondence to R. M. Sundaram or M. S. Prasad.

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Funding

This study was funded by Department of Biotechnology (DBT) (Grant number: BT/PR11705/AGR/02/646/2008).

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These authors do not have any conflict of interest.

Additional information

V. Abhilash Kumar, C. H. Balachiranjeevi and S. Bhaskar Naik contributed equally to this work.

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Kumar, V.A., Balachiranjeevi, C.H., Naik, S.B. et al. Marker-assisted introgression of the major bacterial blight resistance gene, Xa21 and blast resistance gene, Pi54 into RPHR-1005, the restorer line of the popular rice hybrid, DRRH3. J. Plant Biochem. Biotechnol. 25, 400–409 (2016). https://doi.org/10.1007/s13562-016-0352-z

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