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Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety

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Abstract

Samba Mahsuri (BPT5204) is a medium slender grain indica rice variety that is very popular with farmers and consumers across India because of its high yield and excellent cooking quality. However, the variety is susceptible to several diseases and pests, including bacterial blight (BB). We have used PCR based molecular markers in a backcross-breeding program to introgress three major BB resistance genes (Xa21, xa13 and xa5) into Samba Mahsuri from a donor line (SS1113) in which all the three genes are present in a homozygous condition. At each backcross generation, markers closely linked to the three genes were used to select plants possessing these resistance genes (foreground selection) and microsatellite markers polymorphic between donor and recurrent parent were used to select plants that have maximum contribution from the recurrent parent genome (background selection). A selected BC4F1 plant was selfed to generate homozygous BC4F2 plants with different combinations of BB resistance genes. The three-gene pyramid and two-gene pyramid lines exhibited high levels of resistance against the BB pathogen. Under conditions of BB infection, the three-gene pyramid lines exhibited a significant yield advantage over Samba Mahsuri. Most importantly, these lines retain the excellent grain and cooking qualities of Samba Mahsuri without compromising the yield as determined in multi-location trials. This work demonstrates the successful application of marker-assisted selection for targeted introgression of multiple resistance genes into a premium quality rice variety.

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Acknowledgements

We thank Drs. A. P. K. Reddy and U. Prasada Rao for discussions. The encouragement and support received from Drs. B. Mishra and B.C. Viraktamath is thankfully acknowledged. The assistance received from Mr. P. Natarajkumar and Mr. D. Surendher Reddy in conducting the field experiments is also acknowledged. This work was supported by a grant from the National Agricultural Technology Project (NATP) of the Indian Council of Agricultural Research. The authors would also like to thank the scientists at the cooperating centers of AICRIP for conducting the field trails.

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Authors and Affiliations

  1. Directorate of Rice Research, Rajendranagar, Hyderabad, 500030, India

    Raman M. Sundaram, Sunil K. Biradar, Gouri S. Laha, Gajjala Ashok Reddy, N. Shobha Rani & Nukala P. Sarma

  2. Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500007, India

    Manne R. Vishnupriya & Ramesh Venkata Sonti

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  1. Raman M. Sundaram
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  2. Manne R. Vishnupriya
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  3. Sunil K. Biradar
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  4. Gouri S. Laha
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  5. Gajjala Ashok Reddy
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  6. N. Shobha Rani
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  7. Nukala P. Sarma
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  8. Ramesh Venkata Sonti
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Correspondence to Ramesh Venkata Sonti.

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R. M. Sundaram and M. R. Vishnupriya have contributed equally to this work.

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Sundaram, R.M., Vishnupriya, M.R., Biradar, S.K. et al. Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica 160, 411–422 (2008). https://doi.org/10.1007/s10681-007-9564-6

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  • DOI: https://doi.org/10.1007/s10681-007-9564-6

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