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Molecular marker-assisted pyramiding of broad-spectrum disease resistance genes, Pi2 and Xa23, into GZ63-4S, an elite thermo-sensitive genic male-sterile line in rice

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Abstract

The two-line system of hybrid rice seed production based on thermo-sensitive genic male-sterile (TGMS) lines is much more cost-effective, simple and efficient than the three-line system with cytoplasmic male sterility. In this study, we report the introgression of a rice blast resistance gene Pi2 from VE6219 and a bacterial blight (BB) resistance gene Xa23 from HBQ810 into Guangzhan63-4S (GZ63-4S), an elite TGMS rice line, through marker-assisted pyramiding, leading to the development of Hua1015S, a blast- and BB-resistant pyramided line. The newly developed TGMS line Hua1015S and its derived hybrids showed resistance to rice blast and BB. Hua1015S had a similar critical temperature point of fertility–sterility alteration to GZ63-4S and similar key agronomic and grain quality traits. However, Hua1015S gave higher hybrid seed yields on account of increased outcrossing rates due to better floral traits, e.g. spikelet dehiscence, angle of glume opening, and stigma exsertion. The newly developed TGMS parental line Hua1015S with Pi2 and Xa23 is currently being utilized for heterosis breeding of broad-spectrum blast- and BB-resistant two-line rice hybrids and as an improved disease donor source for further TGMS parental line improvement.

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References

  • Chen S, Li XH, Xu CG, Zhang QF (2000) Improvement of bacterial blight resistance of ‘Minghui 63’, an elite restorer line of hybrid rice, by molecular marker-assisted selection. Crop Sci 40:239–244

    Article  Google Scholar 

  • Chen HQ, Chen ZX, Ni S, Zuo SM, Pan XB, Zhu XD (2008) Pyramiding three genes with resistance to blast by marker assisted selection to improve rice blast resistance of Jin 23B. Chin J Rice Sci 22:23–27

    CAS  Google Scholar 

  • Chu Z, Yuan M, Yao J, Ge X, Yuan B, Xu C, Li X, Fu B, Li Z, Bennetzen JL, Zhang Q, Wang S (2006) Promoter mutations of an essential gene for pollen development result in disease resistance in rice. Gene Dev 20:1250–1255

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dai Z, Liu G, Li A, Xu M, Liu X, Zhou C, Zhang H (2005) Breeding of two-line indica hybrid rice combination, “Yangliangyou 6”, and studying on its culture characteristics. Chin Agric Sci Bull 21:114–116

    Google Scholar 

  • Dellaporta SL, Woo J, Hicks JB (1983) A plant DNA mini preparation: version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Deng YW, Zhu XD, Shen Y, He ZH (2006) Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety. Theor Appl Genet 113:705–713

    Article  CAS  PubMed  Google Scholar 

  • Fan YR, Zhang QF (2014) Understanding a key gene for thermosensitive genic male sterility in rice. Sci China Life Sci 57:1241–1242

    Article  PubMed  Google Scholar 

  • Fukuoka S, Saka N, Koga H, Ono K, Shimizu T, Ebana K, Hayashi N, Takahashi A, Hirochika H, Okuno K, Yano M (2009) Loss of function of a proline-containing protein confers durable disease resistance in rice. Science 325:998–1001

    Article  CAS  PubMed  Google Scholar 

  • Gao LG, Gao HL, Li RB, Li DY, Zhou M, Yan Q, Zhou WY, Zhang J, Deng GF (2010) Optimization and verification of molecular for rice bacterial blight resistance gene Xa23. Mol Plant Breed 8:660–664

    CAS  Google Scholar 

  • Hayashi K, Hashimoto N, Daigen M, Ashikawa I (2004) Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus. Theor Appl Genet 108:1212–1220

    Article  CAS  PubMed  Google Scholar 

  • Hittalmani S, Parco A, Mew TV, Zeigler RS, Huang N (2000) Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theor Appl Genet 100:1121–1128

    Article  CAS  Google Scholar 

  • Huang B, Xu JY, Hou MS, Ali J, Mou TM (2012) Introgression of bacterial blight resistance genes Xa7, Xa21, Xa22 and Xa23 into hybrid rice restorer lines by molecular marker-assisted selection. Euphytica 187:449–459

    Article  CAS  Google Scholar 

  • IRRI (2002) Standard evaluation system for rice (SES). International Rice Research Institute, Los Banos

    Google Scholar 

  • Ji GH, Zhang SG, Qian J (2003) Preliminary analysis on races of Xanthomonas oryzae pv. oryzae in Yunnan Province. Plant Prot 29:19–21

    Google Scholar 

  • Jiang HC, Feng YT, Bao L, Li X, Gao GJ, Zhang QL, Xiao JH, Xu CG, He YQ (2012) Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding. Mol Breed 30:1679–1688

    Article  CAS  Google Scholar 

  • Khush GS (2001) Green revolution: the way forward. Nat Rev Genet 2:815–822

    Article  CAS  PubMed  Google Scholar 

  • Li CY, Wei J, Lin YJ, Chen H (2012) Gene silencing using the recessive rice bacterial blight resistance gene xa13 as a new paradigm in plant breeding. Plant Cell Rep 31:851–862

    Article  CAS  PubMed  Google Scholar 

  • Liu G, Lu G, Zeng L, Wang GL (2002) Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Mol Genet Genomics 267:472–480

    Article  CAS  PubMed  Google Scholar 

  • Liu H, Yang W, Hu B, Liu F (2007) Virulence analysis and race classification of Xanthomonas oryzae pv. oryzae in China. J Phytopathol 155:129–135

    Article  CAS  Google Scholar 

  • Liu J, Wang X, Mitchell T, Hu Y, Liu X, Dai L, Wang GL (2010) Recent progress and understanding of the molecular mechanisms of the rice–Magnaporthe oryzae interaction. Mol Plant Pathol 11:419–427

    Article  CAS  PubMed  Google Scholar 

  • Luo YC, Yin ZC (2013) Marker-assisted breeding of Thai fragrance rice for semidwarf phenotype, submergence tolerance and disease resistance to rice blast and bacterial blight. Mol Breed 32:709–721

    Article  CAS  Google Scholar 

  • Luo YC, Sangha JS, Wang SH, Li ZF, Yang JB, Yin ZC (2012) Marker-assisted breeding of Xa4, Xa21 and Xa27 in the restorer lines of hybrid rice for broad-spectrum and enhanced disease resistance to bacterial blight. Mol Breed 30:1601–1610

    Article  CAS  Google Scholar 

  • Mackill DJ, Bonman JM (1992) Inheritance of blast resistance in near-isogenic lines of rice. Phytopathology 82:746–749

    Article  Google Scholar 

  • Maruthasalam S, Kalpana K, Kumar KK, Loganathan M, Poovannan K, Raja JAJ, Kokiladevi E, Samiyappan R, Sudhakar D, Balasubramanian P (2007) Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight. Plant Cell Rep 26:791–804

    Article  CAS  PubMed  Google Scholar 

  • Ou SH (1985) Rice diseases, 2nd edn. Commonwealth Mycological Institute, Richmond

    Google Scholar 

  • Skamnioti P, Gurr SJ (2009) Against the grain: safeguarding rice from rice blast disease. Trends Biotechnol 27:141–150

    Article  CAS  PubMed  Google Scholar 

  • Song WY, Wang GL, Chen LL, Kim HS, Pi LY, Holsten T, Gardner J, Wang B, Zhai WX, Zhu LH, Fauquet C, Ronald P (1995) A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science 270:1804–1806

    Article  CAS  PubMed  Google Scholar 

  • Sundaram RM, Vishnupriya RM, Biradar SK, Laha GS, Ashok Reddy G, Shobha Rani N, Sarma P, Sonti RV (2008) Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica 160:411–422

    Article  Google Scholar 

  • Virmani SS, Sun ZX, Mou TM, Ali J, Mao CX (2003) Two-line hybrid rice breeding manual. International Rice Research Institute, Los Banos

    Google Scholar 

  • Wang ZH, Zou YJ, Li XY, Zhang QY, Chen L, Wu H, Su DH, Chen YL, Guo JX, Luo D, Long YM, Zhong Y, Liu YG (2006) Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell 18:676–687

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Wang CL, Zhang XP, Fan YL, Gao Y, Zhu QL, Zheng CK, Qin TF, Li YQ, Che JY, Zhang MW, Yang B, Liu YG, Zhao KJ (2015) XA23 is an executor R protein and confers broad-spectrum disease resistance in rice. Mol Plant 8:290–302. doi:10.1093/mp/ssu132

    Article  PubMed  Google Scholar 

  • Xu JJ, Wang BH, Wu YH, Du PN, Wang J, Wang M, Yi CD, Gu MH, Liang GH (2011) Fine mapping and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.). Theor Appl Genet 122:365–372

    Article  PubMed  Google Scholar 

  • Yang QK, Liang CY, Zhuang W, Li J, Deng HB, Deng QY, Wang B (2007) Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping. Planta 225:321–330

    Article  CAS  PubMed  Google Scholar 

  • Yi G, Lee SK, Hong YK, Cho YC, Nam MH, Kim SC, Han SS, Wang GL, Hahn TR, Ronald PC, Jeon JS (2004) Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea. Theor Appl Genet 109:978–985

    Article  CAS  PubMed  Google Scholar 

  • Yuan L (2004) Hybrid rice technology for food security in the world. Crop Res 18:185–186

    CAS  Google Scholar 

  • Zeng LX, Huang SH, Wu SZ (2002) The resistance of IRBB21 (Xa21) against 5 races of Guangdong Province. Acta Phytopathol Sin 29:97–100

    Google Scholar 

  • Zhang Q (2009) Genetics and improvement of bacterial blight resistance of hybrid rice in China. Rice Sci 16:83–92

    Article  Google Scholar 

  • Zhang Q, Lin SC, Zhao BY, Wang CL, Yang WC, Zhou YL, Li DY, Chen CB, Zhu LH (1998) Identification and tagging a new gene for resistance to bacterial blight (Xanthomonas oryzae pv. oryzae) from O. rufipogon. Rice Genet Newsl 15:138–142

    Google Scholar 

  • Zhang Q, Wang CL, Zhao KJ, Zhao YL, Caslana VC, Zhu XD, Li DY, Jiang QX (2001) The effectiveness of advanced rice lines with new resistance gene Xa23 to rice bacterial blight. Rice Genet Newsl 18:71–72

    CAS  Google Scholar 

  • Zhou B, Qu S, Liu G, Dolan M, Sakai H, Lu G, Bellizzi M, Wang GL (2006) The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol Plant Microbe Interact 19:1216–1228

    Article  CAS  PubMed  Google Scholar 

  • Zhou YL, Xu JL, Zhou SC, Yu J, Xie XW, Xu MR, Sun Y, Zhu LH, Fu BY, Gao YM, Li ZK (2009) Pyramiding Xa23 and Rxo1 for resistance to two bacterial diseases into an elite indica rice variety using molecular approaches. Mol Breed 23:279–287

    Article  CAS  Google Scholar 

  • Zhou YL, Uzokwe VNE, Zhang CH, Cheng LR, Wang L, Chen K, Gao XQ, Sun Y, Chen JJ, Zhu LH, Zhang Q, Ali J, Xu JL, Li ZK (2011) Improvement of bacterial blight resistance of hybrid rice in China using the Xa23 gene derived from wild rice (Oryza rufipogon). Crop Prot 30:637–644

    Article  CAS  Google Scholar 

  • Zhou H, Zhou M, Yang YZ, Li J, Zhu LY, Jiang DG, Dong JF, Liu QJ, Gu LF, Zhou LY, Feng MJ, Qin P, Hu XC, Song CL, Shi JF, Song XW, Ni ED, Wu XJ, Deng QY, Liu ZL, Chen MS, Liu YG, Cao XF, Zhuang CX (2014) RNase ZS1 processes Ub L40 mRNAs and controls thermosensitive genic male sterility in rice. Nat Commun 5:4884–4892

    Article  CAS  PubMed  Google Scholar 

  • Zhu X, Chen S, Yang J, Zhou S, Zeng L, Han J, Su J, Wang L, Pan Q (2012) The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family. Theor Appl Genet 124:1295–1304

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The research work was supported by Grants from the National High Technology (863) Plan of China (2014AA10A604), Key Technology Research Project of Wuhan City, China (2014020202010136) and the Bill & Melinda Gates Foundation “Green Super Rice for the Resources-Poor of Africa and Asia”.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Jiefeng Jiang, Dabing Yang & Tongmin Mou

  2. International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

    Jauhar Ali

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  1. Jiefeng Jiang
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  2. Dabing Yang
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  3. Jauhar Ali
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  4. Tongmin Mou
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Correspondence to Jauhar Ali or Tongmin Mou.

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Jiang, J., Yang, D., Ali, J. et al. Molecular marker-assisted pyramiding of broad-spectrum disease resistance genes, Pi2 and Xa23, into GZ63-4S, an elite thermo-sensitive genic male-sterile line in rice. Mol Breeding 35, 83 (2015). https://doi.org/10.1007/s11032-015-0282-9

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