Abstract
Rice is a staple food crop for more than half of the world’s population. However, rice production is affected by many types of abiotic and biotic stress. Genetic breeding by utilizing natural resistance or tolerance genes is the most economic and efficient way to combat or adapt to these stresses. Khao Dawk Mali 105 (KDML 105) is an elite cultivar of aromatic rice mainly grown in Thailand. However, the production of KDML 105 is affected by lodging problems due to its tall plant type, regular flash floods or short-term submergence during the monsoon season, and diseases such as blast and bacterial blight. Here we report the pyramiding of semi-dwarf gene sd1, submergence tolerance gene Sub1A, blast resistance gene Pi9 and bacterial blight resistance genes Xa21 and Xa27 in KDML 105 by marker-assisted selection. The improved line, designated T5105, has a semi-dwarf phenotype with improved lodging resistance and a greater harvest index. T5105 survives after 2 weeks of complete submergence without significant loss of viability. T5105 confers high resistance to all five Magnaporthe oryzae isolates tested and provides resistance or moderate resistance to 25 of the 27 Xanthomonas oryzae pv. oryzae strains tested. In addition, T5105 produced higher yield than KDML 105 in two field trials and retains similar good grain quality to KDML 105. The development of T5105 provides a new line to boost the production of high-quality aromatic rice in tropical regions.
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References
Amante-Bordeos A, Sitch L, Nelson R, Dalmacio R, Oliva N, Aswidinnoor H, Leung H (1992) Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice, Oryza sativa. Theor Appl Genet 84:345–354
Bradbury LM, Fitzgerald TL, Henry RJ, Jin Q, Waters DL (2005) The gene for fragrance in rice. Plant Biotechnol J 3:363–370
Chen S, Lin X, Xu C, Zhang Q (2000) Improvement of bacterial blight resistance of ‘Minghui 63’, an elite restorer line of hybrid rice, by molecular marker-assisted selection. Crop Sci 40:239–244
Cruz ND, Khush GS (2000) Rice grain quality evaluation procedures. In: Singh RK, Singh US, Khush GS (eds) Aromatic rices. Oxford and IBH Publishing Co. Pvt. Ltd, New Delhi, pp 24–36
Datta K, Baisakh N, Thet KM, Tu J, Datta SK (2002) Pyramiding transgenes for multiple resistance in rice against bacterial blight, yellow stem borer and sheath blight. Theor Appl Genet 106:1–8
Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1–42
Fukao T, Yeung E, Bailey-Serres J (2011) The submergence tolerance regulator SUB1A mediates crosstalk between submergence and drought tolerance in rice. Plant Cell 23:412–427
Gnanamanickam S, Priyadarisini VB, Narayanan N, Vasudevan P, Kavitha S (1999) An overview of bacterial blight disease of rice and strategies for its management. Curr Sci 77:1435–1444
Gu K, Tian D, Yang F, Wu L, Sreekala C, Wang D, Wang GL, Yin Z (2004) High-resolution genetic mapping of Xa27 (t), a new bacterial blight resistance gene in rice, Oryza sativa L. Theor Appl Genet 108:800–807
Hedden P (2003) The genes of the Green Revolution. Trends Genet 19:5–9
Huang N, Angeles ER, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bennett J, Khush GS (1997) Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theor Appl Genet 95:313–320
Jirawut WA (2012) Increasing rice production: solution to the global food crisis. http://www.nodai.ac.jp/cip/iss/english/9th_iss/fullpaper/1-2-2ku-jirawut.pdf. Accessed 31 Dec 2012
Kauffman H, Reddy A, Hsieh S, Merca S (1973) An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep 57:537–541
Koide Y, Ebron LA, Kato H, Tsunematsu H, Telebanco-Yanoria MJ, Kobayashi N, Yokoo M, Maruyama S, Imbe T, Fukuta Y (2011) A set of near-isogenic lines for blast resistance genes with an Indica-type rainfed lowland elite rice (Oryza sativa L.) genetic background. Field Crop Res 123:19–27
Kottapalli KR, Narasu ML, Jena KK (2010) Effective strategy for pyramiding three bacterial blight resistance genes into fine grain rice cultivar, Samba Mahsuri, using sequence tagged site markers. Biotechnol Lett 32:989–996
Lanceras JC, Huang ZL, Naivikul O, Vanavichit A, Ruanjaichon V, Tragoonrung S (2000) Mapping of genes for cooking and eating qualities in Thai jasmine rice (KDML105). DNA Res 7:93–101
Liu G, Lu G, Zeng L, Wang GL (2002) Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Mol Genet Genomics 267:472–480
Liu J, Wang X, Mitchell T, Hu Y, Liu X, Dai L, Wang GL (2010) Recent progress and understanding of the molecular mechanisms of the rice—Magnaporthe oryzae interaction. Mol Plant Pathol 11:419–427
Luo Y, Sangha JS, Wang S, Li Z, Yang J, Yin Z (2012) Marker-assisted breeding of Xa4, Xa21 and Xa27 in the restore lines of hybrid rice for broad-spectrum and enhanced disease resistance to bacterial blight. Mol Breed 30:1601–1610
Neeraja CN, Maghirang-Rodriguez R, Pamplona A, Heuer S, Collard BC, Septiningsih EM, Vergara G, Sanchez D, Xu K, Ismail AM, Mackill DJ (2007) A marker-assisted backcross approach for developing submergence-tolerant rice cultivars. Theor Appl Genet 115:767–776
Nettuwakul C, Pongtongkam P, Thongpan A, Peyachoknagul S (2007) Detection of photoperiod responsive gene in KDML 105 rice (Oryza sativa L.) using cDNA-SRAP technique. Kasetsart J (Nat Sci) 41:651–659
Nino-Liu DO, Ronald PC, Bogdanove AJ (2006) Xanthomonas oryzae pathovars: model pathogens of a model crop. Mol Plant Pathol 7:303–324
Perez LM, Redona ED, Mendioro MS, Cruz CMV, Leung H (2008) Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice (Oryza sativa L.) for the development of two-line hybrids. Euphytica 164:627–636
Pitiphunpong S, Suwannaporn P (2009) Physicochemical properties of KDML 105 rice cultivar from different cultivated locations in Thailand. J Sci Food Agric 89:2186–2190
Qu S, Liu G, Zhou B, Bellizzi M, Zeng L, Dai L, Han B, Wang GL (2006) The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics 172:1901–1914
Rajpurohit D, Kumar R, Kumar M, Paul P, Awasthi A, Basha PO, Puri A, Jhang T, Singh K, Dhaliwal HS (2011) Pyramiding of two bacterial blight resistance and a semidwarfing gene in Type 3 Basmati using marker-assisted selection. Euphytica 178:111–126
Roongsattham P, Pongtongkam P, Thongpan A, Kaveeta L, Harinasut P, Peyachoknagul S (2006) Hd1, Hd3a, and Hd6 genes: possible DNA methylation roles in photoperiod sensitive gene regulation of rice KDML 105 (Oryza sativa L.). Kasetsart J (Nat Sci) 40:462–471
Sarkarung S, Somrith B, Chitrakorn S (2000) Aromatic rice of Thailand. In: Singh R, Singh U, Khush G (eds) Aromatic rices. Oxford and IBH Publishing Co. Pvt. Ltd, New Delhi, pp 180–183
Singh S, Sidhu JS, Huang N, Vikal Y, Li Z, Brar DS, Dhaliwal HS, Khush GS (2001) Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106. Theor Appl Genet 102:1011–1015
Somrith B (1996) Khao Dawk Mali 105: Problems, research efforts and future prospects. Report of the INGER Monitoring Visit on Fine-Grain Aromatic Rice in India, Iran, Pakistan and Thailand. IRRI, Manila, Philippines, pp 102–111
Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci USA 99:9043–9048
Wang GL, Song WY, Ruan DL, Sideris S, Ronald PC (1996) The cloned gene, Xa21, confers resistance to multiple Xanthomonas oryzae pv. oryzae isolates in transgenic plants. Mol Plant Microbe Interact 9:850–855
Xu KN, Mackill DJ (1996) A major locus for submergence tolerance mapped on rice chromosome 9. Mol Breed 2:219–224
Xu K, Xu X, Fukao T, Canlas P, Maghirang-Rodriguez R, Heuer S, Ismail AM, Bailey-Serres J, Ronald PC, Mackill DJ (2006) Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice. Nature 442:705–708
Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y (2000) Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12:2473–2483
Zhai WX, Wang WM, Zhou YL, Li XB, Zheng XW, Zhang Q, Wang GL, Zhu LH (2002) Breeding bacterial blight-resistant hybrid rice with the cloned bacterial blight resistance gene Xa21. Mol Breed 8:285–293
Zhang J, Li X, Jiang G, Xu Y, He Y (2006) Pyramiding of Xa7 and Xa21 for the improvement of disease resistance to bacterial blight in hybrid rice. Plant Breed 125:600–605
Acknowledgments
We wish to thank D. Tian, K. Vilayheuang, S. Simeuang, S. Du, S. Zakaria, B. Basah and A. Endrajaya for technical assistance, S. Wang for KDML 105, D. J. Mackill for IR64(Sub1ASub1A) and G. Liu for 75-1-127. This research is supported by Temasek Foundation and the National Research Foundation Singapore under its Competitive Research Programme (CRP Award No. NRF-CRP7-2010-02).
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Luo, Y., Yin, Z. Marker-assisted breeding of Thai fragrance rice for semi-dwarf phenotype, submergence tolerance and disease resistance to rice blast and bacterial blight. Mol Breeding 32, 709–721 (2013). https://doi.org/10.1007/s11032-013-9904-2
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DOI: https://doi.org/10.1007/s11032-013-9904-2