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Pyramiding Xa23 and Rxo1 for resistance to two bacterial diseases into an elite indica rice variety using molecular approaches

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Abstract

Rice bacterial leaf blight (BB) caused by Xanthomonas oryzae pv. oryzae and bacterial leaf streak (BLS) caused by X. oryzae pv. oryzicola (Xoc) are two important diseases of rice that often outbreak simultaneously and constrain rice production in much of Asia and parts of Africa. Developing resistant cultivars has been the most effective approach to control BB, however, most single resistance genes have limited value in breeding programs because of their narrow-spectrum of resistance to the races of the pathogen. By contrast, there is little progress in breeding varieties resistant to Xoc since BLS resistance in rice was a quantitative trait and so far only a few quantitative resistance loci have been identified. We reported here the development of a high yield elite line, Lu-You-Zhan highly resistant to both BB and BLS by pyramiding Xa23 with a wide-spectrum resistance to BB derived from wild rice and a non-host maize resistance gene, Rxo1, using both marker assisted selection (MAS) and genetic engineering. Our study has provided strong evidence that non-host R genes could be a valuable source of resistance in combating those plant diseases where no single R gene controlling high level of resistance exists and demonstrated that MAS combined with transgenic technologies are an effective strategy to achieve high level of resistance against multiple plant diseases.

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Acknowledgments

The authors are grateful to the National Natural Science Foundation of China (No. 30571200) and the Ministry of Agriculture (948 # 2006-G51 for providing the financial support. We acknowledge Prof. SH Hulbert, Kansas State University, USA for providing us with plasmid pCAMBIA1305-1 containing Rxo1.

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Authors and Affiliations

  1. Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, 12 South Zhong-Guan-Cun Street, 100081, Beijing, China

    Yong-Li Zhou, Jian-Long Xu, Jing Yu, Xue-Wen Xie, Mei-Rong Xu, Yong Sun, Ling-Hua Zhu, Bin-Ying Fu, Yong-Ming Gao & Zhi-Kang Li

  2. Institute of Rice Research, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, 510640, Guangzhou, China

    Shao-Chuan Zhou

  3. International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

    Zhi-Kang Li

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  1. Yong-Li Zhou
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  2. Jian-Long Xu
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Correspondence to Zhi-Kang Li.

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Y-L Zhou and J-L Xu contributed equally to this work.

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Zhou, YL., Xu, JL., Zhou, SC. et al. Pyramiding Xa23 and Rxo1 for resistance to two bacterial diseases into an elite indica rice variety using molecular approaches. Mol Breeding 23, 279–287 (2009). https://doi.org/10.1007/s11032-008-9232-0

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