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Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping

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Abstract

Previous research has demonstrated that the thermo-sensitive genic male-sterile (TGMS) gene in rice was regulated by temperature. TGMS rice is important to hybrid rice production because the application of the TGMS system in two-line breeding is cost-effective, simple, efficient and overcomes the limitations of the cytoplasmic male sterility (CMS) system. AnnongS is the first discovered and deeply studied TGMS rice line in China. Previous studies have suggested that AnnongS-1 and Y58S, two derivative TGMS lines of AnnongS, were both controlled by a single recessive gene named tms5, which was genetically mapped on chromosome 2. In the current study, three populations (AnnongS-1 × Nanjing11, Y58S × Q611, and Y58S × Guanghui122) were developed to investigate the tms5 gene molecular map. Analysis of recombination events of sterile samples, utilizing 125 probes covering the tms5 region, suggested that the tms5 gene was physically mapped to a 19 kb DNA fragment between two markers, 4039-1 and 4039-2, located on the BAC clone AP004039. Following the construction of a physical map between the two markers, ONAC023, a member of the NAC (NAM-ATAF-CUC-related) gene family, was identified as the candidate of the tms5 gene.

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Abbreviations

TGMS:

Thermo-sensitive genic male-sterile

CMS:

Cytoplasmic male sterility

NAC:

NAM-ATAF-CUC-related

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Acknowledgements

This work was supported by grants from the State Key Basic Research and Development Plan of China (Grant No. 2001CB108802) and the Hi-Tech and Development Program of China (No. 2002AA2Z1001).

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Authors and Affiliations

  1. The State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Qingkai Yang, Chunyang Liang, Jun Li & Bin Wang

  2. The Graduate School of Chinese Academy of Sciences, Beijing , 100039, China

    Qingkai Yang, Chunyang Liang & Jun Li

  3. Hunan Hybrid Rice Research Center, Changsha, 410125, China

    Wen Zhuang, Huabing Deng & Qiyun Deng

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  1. Qingkai Yang
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  2. Chunyang Liang
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  6. Qiyun Deng
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  7. Bin Wang
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Correspondence to Bin Wang.

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Yang, Q., Liang, C., Zhuang, W. et al. Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping. Planta 225, 321–330 (2007). https://doi.org/10.1007/s00425-006-0353-6

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  • DOI: https://doi.org/10.1007/s00425-006-0353-6

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