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The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family

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Abstract

The deployment of broad-spectrum resistance genes is the most effective and economic means of controlling blast in rice. The cultivar Er-Ba-Zhan (EBZ) is a widely used donor of blast resistance in South China, with many cultivars derived from it displaying broad-spectrum resistance against blast. Mapping in a set of recombinant inbred lines bred from the cross between EBZ and the highly blast-susceptible cultivar Liangjiangxintuanheigu (LTH) identified in EBZ a blast resistance gene on each of chromosomes 1 (Pish), 6 (Pi2/Pi9) and 12 (Pita/Pita-2). The resistance spectrum and race specificity of the allele at Pi2/Pi9 were both different from those present in other known Pi2/Pi9 carriers. Fine-scale mapping based on a large number of susceptible EBZ × LTH F2 and EBZ × LTH BC1F2 segregants placed the gene within a 53-kb segment, which includes Pi2/Pi9. Sequence comparisons of the LRR motifs of the four functional NBS-LRR genes within Pi2/Pi9 revealed that the EBZ allele is distinct from other known Pi2/Pi9 alleles. As a result, the gene has been given the designation Pi50(t).

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Acknowledgments

This research was supported by grants from the Earmarked Fund for Modern Agro-Industry Technology Research System, the National 973 project, the National Natural Science Foundation, the National Transgenic Research Projects, the State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and the President Science Foundation of the Guangdong Academy of Agricultural Sciences.

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Authors and Affiliations

  1. Laboratory of Plant Resistance and Genetics, College of Resources and Environmental Sciences, South China Agricultural University, Guangzhou, 510642, China

    Xiaoyuan Zhu, Ling Wang & Qinghua Pan

  2. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, Guangdong, China

    Xiaoyuan Zhu, Shen Chen, Jianyuan Yang, Liexian Zeng, Jingluan Han & Jing Su

  3. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China

    Shaochuan Zhou

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  1. Xiaoyuan Zhu
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Correspondence to Qinghua Pan.

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Communicated by P. Hayes.

X. Zhu and S. Chen contributed equally to this work.

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122_2012_1787_MOESM1_ESM.doc

Fig. S1 Amino acid alignment of the LRR motifs in rice cultivars carrying various Pi2/9 alleles. EBZ carries Pi50(t), C101A51 Pi2, 75-1-27 Pi9, IRBLz-FU Piz, IRBLzt-T Piz-t, Gumei2 Pi26(t), Gumei4 Pigm(t) and IR65482-4-136-2-2 Pi40(t). a, b, c, d NBS-LRR1, NBS-LRR2 (Pi9 ortholog), NBS-LRR4 (Pi2 ortholog) and NBS-LRR7, respectively (DOC 17484 kb)

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Zhu, X., Chen, S., Yang, J. et al. The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family. Theor Appl Genet 124, 1295–1304 (2012). https://doi.org/10.1007/s00122-012-1787-9

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