Skip to main content
SpringerLink
Log in

Allelic variation and distribution independence of Puroindoline b-B2 variants and their association with grain texture in wheat

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

In this study, we identify the allelic variation of the Pinb-B2v3 variant, which could be divided into three different alleles, Pinb-B2v3a, Pinb-B2v3b and Pinb-B2v3c. The result of χ2 tests showed that the distribution of Puroindoline b-2 variants has different frequencies in common and durum wheats. Analysis of the association of Pinb-B2v with grain hardness indicated that wheat cultivars with Pinb-B2v3b possessed relatively higher single kernel characterization system (SKCS) hardness indices in soft wheat in the 2006–2007 cropping season. Further analysis of SKCS hardness among different Puroindoline B-b2 variants by an F8 recombinant inbred line (RIL) population containing 350 RILs indicated that lines with Pinb-2v3b were on average 5.4 SKCS hardness index units harder than those carrying the Pinb-2v2 haplotype. Derived cleaved amplified polymorphic sequence markers were developed for identification of Pinb-B2v3b and Pinb-B2v3c alleles and will be useful for screening early generation materials by marker-assisted selection during wheat breeding. The results of quantitative real-time PCR indicated that the relative expression level of Pinb-B2v3b was significantly higher than those of Pinb-B2v2, Pinb-B2v3a and Pinb-B2v3c, that four Pinb-B2 alleles showed the highest relative expression level on the 14th day after anthesis during grain development, and that relative expression levels of Pinb-B2v3b and Pinb-B2v2 in leaf were significantly higher than those in root, suggesting that PINB-2 are possibly not seed-specific proteins and that the expression level of Pinb-B2v3 was possibly positively correlated with grain hardness.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

PCR:

Polymerase chain reaction

Pinb-2v :

Puroindoline b-2 variants

dCAPS:

Derived cleaved amplified polymorphic sequence

QTL:

Quantitative trait locus

SKCS:

Single kernel characterization system

RIL:

Recombinant inbred line

References

  • Bhave M, Morris CF (2008a) Molecular genetics of puroindolines and related genes: allelic diversity in wheat and other grasses. Plant Mol Biol 66:205–219

    Article  PubMed  CAS  Google Scholar 

  • Bhave M, Morris CF (2008b) Molecular genetics of puroindolines and related genes: regulation of expression, membrane binding properties and applications. Plant Mol Biol 66:221–231

    Article  PubMed  CAS  Google Scholar 

  • Bihan TL, Blochet JE, Desormeaux A, Marion D, Pezolet M (1996) Determination of the secondary structure and conformation of puroindolines by infrared and Raman spectroscopy. Biochemistry 35:12712–12722

    Article  PubMed  Google Scholar 

  • Chen M, Wilkinson M, Tosi P, He G, Shewry P (2005) Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes. Theor Appl Genet 111:1159–1166

    Article  PubMed  CAS  Google Scholar 

  • Chen F, He ZH, Chen DS, Zhang CL, Xia XC (2007) Influence of puroindoline allele on milling, steamed bread, noodles and pan bread in common spring wheat. J Cereal Sci 45:59–66

    Article  CAS  Google Scholar 

  • Chen F, Beecher B, Morris CF (2010a) Physical mapping and a new variant of Puroindoline b-2 genes in wheat. Theor Appl Genet 120:745–751

    Article  PubMed  Google Scholar 

  • Chen F, Zhang FY, Cheng XY, Morris CF, Xu HX, Dong ZD, Zhan KH, Cui DQ (2010b) Association of Puroindoline b-B2 variants with grain traits, yield components and flag leaf size in bread wheat (Triticum aestivum L.) varieties of the Yellow and Huai Valleys of China. J Cereal Sci 52:247–253

    Article  CAS  Google Scholar 

  • Chen F, Zhang FY, Morris CF, He ZH, Xia XC, Cui DQ (2010c) Molecular characterization of puroindoline a-D1b genotype and development of STS marker in bread wheat (Triticum aestivum L.). J Cereal Sci 52:80–82

    Article  CAS  Google Scholar 

  • Chen F, Xu HX, Zhang FY, Xia XC, He ZH, Wang DW, Dong ZD, Zhan KH, Cheng XY, Cui DQ (2011) Physical mapping of puroindoline b-2 genes and molecular characterization of a novel variant in durum wheat (Triticum turgidum L.). Mol Breed 28:153–161

    Article  Google Scholar 

  • Chen F, Zhang FY, Xia XC, Dong ZD, Cui DQ (2012) Distribution of puroindoline alleles in bread wheat cultivars of the Yellow and Huai valley of China and discovery of a novel puroindoline a allele without PINA protein. Mol Breed 29:371–378

    Article  CAS  Google Scholar 

  • Dhatwalia VK, Sati OP, Tripathi MK, Kumar A (2011) Puroindoline: antimicrobial wheat endosperm specific protein. J Agric Tech 7:903–906

    Google Scholar 

  • Gautier MF, Aleman ME, Guirao A, Marion D, Joudrier P (1994) Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and development gene expression. Plant Mol Biol 25:43–51

    Article  PubMed  CAS  Google Scholar 

  • Gautier MF, Cosson P, Guirao A, Alary R, Joudrier P (2000) Puroindoline genes are highly conserved in diploid ancestor wheats and related species but absent in tetraploid Triticum species. Plant Sci 153:81–91

    Article  CAS  Google Scholar 

  • Geng HW, Beecher BS, He ZH, Kiszonas AM, Morris CF (2012a) Prevalence of Puroindoline D1 and Puroindoline b-2 variants in US Pacific Northwest wheat breeding germplasm pools, and their association with kernel texture. Theor Appl Genet 124:1259–1269

    Article  PubMed  CAS  Google Scholar 

  • Geng HW, Beecher BS, He ZH, Morris CF (2012b) Physical mapping of Puroindoline b-2 genes in wheat using ‘Chinese Spring’ chromosome group 7 deletion lines. Crop Sci 52:2674–2678

    Article  CAS  Google Scholar 

  • Geng HW, Beecher BS, Pumphrey M, He ZH, Morris CF (2013) Segregation analysis indicates that Puroindoline b-2 variants 2 and 3 are allelic in Triticum aestivum and that a revision to Puroindoline b-2 gene symbolization is indicated. J Cereal Sci 57:61–66

    Article  CAS  Google Scholar 

  • Gollan P, Smith K, Bhave M (2007) Gsp-1 genes comprise a multigene family in wheat that exhibits a unique combination of sequence diversity yet conservation. J Cereal Sci 45:184–198

    Article  CAS  Google Scholar 

  • Goymer P (2007) Synonymous mutations break their silence. Nat Rev Genet 8:92

    CAS  Google Scholar 

  • Kimchi-Sarfaty C, Oh JM, Kim IW, Sauna ZE, Calcagno AM, Ambudkar SV, Gottesman MM (2007) A “silent” polymorphism in the MDR1 gene changes substrate specificity. Science 315:525–528

    Article  PubMed  CAS  Google Scholar 

  • Ma DY, Zhang Y, Xia XC, Morris CF, He ZH (2009) Milling and Chinese raw white noodle qualities of common wheat near-isogenic lines differing in puroindoline b allele. J Cereal Sci 50:126–130

    Article  Google Scholar 

  • Mohler V, Schmolke M, Paladey E, Seling S, Hart L (2012) Association analysis of Puroindoline-D1 and Puroindoline b-2 loci with 13 quality traits in European winter wheat (Triticum aestivum L.). J Cereal Sci 56(3):623–628

  • Morris CF, Bhave M (2008) Reconciliation of D-genome puroindoline allele designations with current DNA sequence data. J Cereal Sci 48:277–287

    Article  CAS  Google Scholar 

  • Neff MM, Neff JD, Chory J, Pepper AE (1998) dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. Plant J 14:387–392

    Article  PubMed  CAS  Google Scholar 

  • Pagani F, Raponi M, Baralle FE (2005a) Synonymous mutations in CFTR exon 12 affect splicing and are not neutral in evolution. Proc Natl Acad USA 102:6368–6372

    Article  CAS  Google Scholar 

  • Pagani F, Raponi M, Baralle FE (2005b) Synonymous mutations in CFTR exon 12 affect splicing and are not neutral in evolution. Proc Natl Acad Sci USA 102:6368–6372

    Article  PubMed  CAS  Google Scholar 

  • Pereira PJ, Lozanov V, Patthy A, Huber R, Bode W, Pongor S, Strobl S (1999) Specific inhibition of insect alpha-amylases: yellow meal worm alpha-amylase in complex with the amaranth alpha-amylase inhibitor at 2.0 A resolution. Struct 15:1079–1088

    Google Scholar 

  • Ramalingam A, Palombo EA, Bhave M (2012) The Pinb-2 genes in wheat comprise a multigene family with great sequence diversity and important variants. J Cereal Sci 56:171–180

    Article  CAS  Google Scholar 

  • Wilkinson M, Wan Y, Tosi P, Leverington M, Snape J, Mitchel RAC, Shewry PR (2008) Identification and genetic mapping of variant forms of puroindoline b expressed in developing wheat grain. J Cereal Sci 48:722–728

    Article  CAS  Google Scholar 

  • Xu HX, Jiang XY, Zhan KH, Cheng XY, Chen XJ, Pardo JM, Cui DQ (2008) Functional characterization of a wheat plasma membrane Na+/H+ antiporter in yeast. Arch Biochem Biophys 473:8–15

    Article  PubMed  CAS  Google Scholar 

  • Zhang J, Martin JM, Beecher B, Morris CF, Hannah LC, Giroux MJ (2009) Seed-specific expression of the wheat puroindoline genes improves maize wet milling yields. Plant Biotechnol J 7:733–743

    Article  PubMed  CAS  Google Scholar 

  • Zhang J, Martin JM, Beecher B, Lu C, Hannah LC, Wall ML, Altosaar I, Giroux MJ (2010) The ectopic expression of the wheat puroindoline genes increase germ size and seed oil content in transgenic corn. Plant Mol Biol 74:353–365

    Article  PubMed  CAS  Google Scholar 

  • Zhang J, Martin JM, Balint-Kurti P, Huang L, Giroux MJ (2011) The wheat puroindoline genes confer fungal resistance in transgenic corn. J Phytopathol 159:188–190

    Article  Google Scholar 

Download references

Acknowledgments

The authors thank Prof. Roberto Tuberosa of University of Bologna for providing the seeds of durum wheat cultivars and Prof. Xia Xianchun of Chinese Academy of Agricultural Sciences for providing cDNA of diverse genotypes in different grain filling stages. This project was funded by the National Natural Science Foundation (31000708), Henan International Science and Technology Cooperation Program (114300510013) and National Science and Technology Pillar Program (2011BAD07B01) of China.

Author information

Authors and Affiliations

  1. Agronomy College, Henan Agricultural University, Wenhua Road 95, Zhengzhou, 450002, People’s Republic of China

    Feng Chen, Fuyan Zhang, Huanhuan Li, Yingying Cao, Xiaoli Shang & Dangqun Cui

  2. Cooperative Innovation Center of Food Crops in Henan Province, Zhengzhou, 450002, China

    Feng Chen & Dangqun Cui

  3. USDA-ARS Western Wheat Quality Laboratory, E-202 Food Science and Human Nutrition Facility East, Washington State University, Pullman, WA, 99164-6394, USA

    Craig F. Morris

Authors
  1. Feng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fuyan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Huanhuan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Craig F. Morris
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yingying Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaoli Shang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dangqun Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Feng Chen or Dangqun Cui.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, F., Zhang, F., Li, H. et al. Allelic variation and distribution independence of Puroindoline b-B2 variants and their association with grain texture in wheat. Mol Breeding 32, 399–409 (2013). https://doi.org/10.1007/s11032-013-9879-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-013-9879-z

Keywords

Search

Navigation