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Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes

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Abstract

DNA from six hexaploid, tetraploid and diploid species of Aegilops with the C, D, S, M and U genomes was amplified with specific PCR primers to identify sequences encoding puroindolines (Pins) a and b and grain softness protein (GSP), all of which are encoded by genes at the Ha (hardness) locus, with Ae. tauschii (DD) and bread wheat (T. aestivum) (AABBDD) cv Hiline being studied as controls. Seven new allelic forms of Pin a and Pin b were identified, including forms with mutations within or close to the tryptophan motif. In addition, five new forms of GSP were detected. In all species both genomic DNA from leaves and cDNA from developing grain were analysed. This revealed the presence of both silent genes (with premature stop codons) and multiple genes, with the latter being confirmed by Southern blot analysis. Freeze fracture analysis demonstrated that all except one accession (Ae. sharonensis) were soft textured. However, this difference cannot be accounted for by the sequences of the Pin alleles present in this line.

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Acknowledgements

Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom. We wish to thank Rothamsted International for providing a Fellowship for M Chen and Dr Nigel Halford for assistance with Table 3. We are also very grateful to Dr Huw Jones and Ms Caroline Sparks for advice and discussions and to Mrs Jean Devonshire for her assistance with the SEM.

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Authors and Affiliations

  1. China-UK HUST-RRes Crop Engineering and Genomics Joint Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Luoyu Road 1037, Wuhan, Hubei, People’s Republic of China

    Mingjie Chen & Guangyuan He

  2. Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK

    Mingjie Chen, Mark Wilkinson, Paola Tosi & Peter Shewry

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  1. Mingjie Chen
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  2. Mark Wilkinson
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  3. Paola Tosi
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  4. Guangyuan He
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  5. Peter Shewry
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Correspondence to Peter Shewry.

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Communicated by P. Langridge

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Chen, M., Wilkinson, M., Tosi, P. et al. Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes. Theor Appl Genet 111, 1159–1166 (2005). https://doi.org/10.1007/s00122-005-0047-7

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  • DOI: https://doi.org/10.1007/s00122-005-0047-7

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