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Identification of novel tan spot resistance QTLs using an SSR-based linkage map of tetraploid wheat

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Abstract

Durum wheat (Triticum turgidum L. subsp. durum, 2n = 4x = 28, AABB) is an important cereal used for making pasta products. Compared with bread wheat, durum wheat receives less attention in genetic and genomic studies. In this research, a tetraploid wheat doubled haploid (DH) population derived from the cross between the durum wheat cultivar ‘Lebsock’ and the T. turgidum subsp. carthlicum (2n = 4x = 28, AABB) accession PI 94749 was developed. The population consisted of 146 lines and was used to construct linkage maps of all 14 chromosomes. The maps consisted of 280 SSR markers and spanned 2,034.1 cM with an average density of one marker per 7.2 cM. The DH population and the whole genome linkage maps were then used to identify QTLs associated with tan spot resistance. The DH population was inoculated separately with two Ptr ToxA-producing isolates (Pti2 and 86-124) representing races 1 and 2, respectively, of Pyrenophora tritici-repentis, and five resistance QTLs were detected on chromosome arms 3AS, 3BL, 5AL and 7BL. Together, the QTLs explained a total of 46 and 41% of the phenotypic variation for reaction to Pti2 and 86-124, respectively. The Tsn1-Ptr ToxA interaction was not a significant factor in tan spot development in this population, and none of the QTLs corresponded to previously identified loci known to confer insensitivity to host-selective toxins (HSTs) produced by P. tritici-repentis. This result, together with those of other similar studies, indicates that the wheat–P. tritici-repentis pathosystem involves more factors than currently published host-toxin interactions. The DH population and genetic maps reported here will be useful for genetic dissection of important agronomic traits as well as the identification and development of markers for marker-assisted selection (MAS).

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Acknowledgements

The authors thank S.W. Meinhardt for providing purified Ptr ToxA. This research was supported by USDA-ARS CRIS Project 5442-22000-033-00D.

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Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58105, USA

    C.-G. Chu & S. Zhong

  2. Cereal Crops Research Unit, Northern Crop Science Laboratory, USDA-ARS, 1307 18th Street North, Fargo, ND, 58105-5677, USA

    S. Chao, T. L. Friesen, J. D. Faris & S. S. Xu

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  1. C.-G. Chu
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  2. S. Chao
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  3. T. L. Friesen
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  4. J. D. Faris
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  5. S. Zhong
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  6. S. S. Xu
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Correspondence to S. S. Xu.

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Chu, CG., Chao, S., Friesen, T.L. et al. Identification of novel tan spot resistance QTLs using an SSR-based linkage map of tetraploid wheat. Mol Breeding 25, 327–338 (2010). https://doi.org/10.1007/s11032-009-9335-2

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  • DOI: https://doi.org/10.1007/s11032-009-9335-2

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