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Evaluation of QTL alleles from exotic sources for hybrid seed yield in the original and different genetic backgrounds of spring-type Brassica napus L.

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Abstract

Previously identified alleles at quantitative trait loci (QTL) for hybrid seed yield were re-evaluated in the same genetic background (in hybrid combination with the same tester) as the original QTL mapping study and also evaluated in a different genetic background (in hybrid combination with two different testers). The QTL were identified from wide crosses of exotic germplasm sources with spring-type Brassica napus L., in which alleles from the exotic germplasm sources increased hybrid seed yield. Results from the re-evaluation of six QTL, in the same genetic background and hybrid combination, indicate that several of the exotic donor QTL alleles did increase hybrid seed yield and could be successfully used for improving the original single-cross hybrid. However, results from the evaluation of seven QTL (including the same six previous QTL) in a new genetic background, in combination with two new testers, indicate that the exotic QTL alleles were often no different or produced significantly lower hybrid seed yield than the spring QTL alleles. In all studies, the QTL were also very sensitive to environmental interactions. Thus, our results indicate that although these exotic sources contain favorable QTL alleles when introgressed into one spring hybrid background, the effects are not predictive of other genetic backgrounds or hybrid combinations. Although QTL affecting hybrid seed yield have been identified, comparisons of multiple QTL alleles are needed to determine the most favorable allele at each locus. Characterization of QTL complementation across testers will be required to predict their effects in multiple hybrid combinations.

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Abbreviations

CI:

Confidence interval

CMS:

Cytoplasmic male sterility

DH:

Double haploid

IBL:

Inbred backcross line

LOD:

Logarithm of odds

MAS:

Marker-assisted selection

QTL:

Quantitative trait loci

RCBD:

Randomized complete block design

RFLP:

Restriction fragment length polymorphism

SI:

Self incompatibility

STS:

Sequence-tagged sites

SSR:

Simple sequence repeats

SSCP:

Single-strand conformation polymorphism

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Acknowledgments

We thank Robert Gaeta, Federico Iniguez-Luy, Ivan Maureira, Joy Muangprom, Chris Pires, Tim Pruski, Pablo Quijada, Dena Tellefsen, Josh Udall, Robert Vogelzang, and Jianwei Zhao for technical assistance and discussion. Funding to TCO was provided by the USDA North Central Canola Research Program. CCK was supported in part by the Gabelman-Shippo Distinguished Graduate Fellowship in Plant Breeding & Plant Genetics and the Pioneer Plant Breeding Graduate Fellowship, University of Wisconsin, Madison.

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  1. Chad C. Kramer & Thomas C. Osborn

    Present address: Seminis Vegetable Seeds, Inc., 37437 State Highway 16, Woodland, CA, 95695, USA

Authors and Affiliations

  1. Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI, 53706, USA

    Chad C. Kramer & Thomas C. Osborn

  2. Bayer CropScience, Inc., Site 600, Box 117, RR. #6, Saskatoon, SK, S7K 3J9, Canada

    Hieronim Polewicz

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  1. Chad C. Kramer
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Correspondence to Chad C. Kramer.

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Kramer, C.C., Polewicz, H. & Osborn, T.C. Evaluation of QTL alleles from exotic sources for hybrid seed yield in the original and different genetic backgrounds of spring-type Brassica napus L.. Mol Breeding 24, 419–431 (2009). https://doi.org/10.1007/s11032-009-9303-x

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