Abstract
Most steps in plant nucleic acid isolation are easily adapted to 96-well format; however, tissue disruption typically is performed on samples individually and often is the rate-limiting step in sample processing. We have found that DNA and RNA isolation from Arabidopsis tissue can be carried out in 96-well format using a paint shaker fitted with an adapter for tissue disruption.
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References
Edwards K, Johnstone C and Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19: 1349.
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Michaels, S.D., Amasino, R.M. High throughput isolation of DNA and RNA in 96-well format using a paint shaker. Plant Mol Biol Rep 19, 227–233 (2001). https://doi.org/10.1007/BF02772894
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DOI: https://doi.org/10.1007/BF02772894