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Genetic analysis of durable resistance against leaf rust in durum wheat

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Abstract

The Italian durum wheat cultivar Creso possesses a high level of durable resistance to leaf rust based on both hypersensitive and non-hypersensitive components. In order to investigate the genetic basis of this resistance, a segregating population composed of 123 recombinant inbred lines (RILs) derived from the cross Creso × Pedroso, was evaluated for disease severity in adult plants under field conditions. Furthermore, the resistance of parents and RILs was evaluated by assessing macroscopically the latency period and microscopically the number and type of pathogen colonies formed following artificial inoculation with a specific isolate. This experiment was performed at controlled conditions at two developmental stages. Besides some minor QTLs, one major QTL explaining both reduction of disease severity in the field and increased latency period was found on the long arm of chromosome 7B, and closely associated PCR-based and DArT markers were identified.

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Abbreviations

Lr:

Leaf rust

HP:

Hypersentitive response

IT:

Infection type

PR:

Partial resistance

LP:

Latency period

MAS:

Marker-assisted selection

RIL:

Recombinant inbred line

RDS:

Relative disease severity

SSR:

Simple sequence repeat

STS:

Sequence tagged site

DArT:

Diversity array technology

LOD:

Logarithm of odds

QTL:

Quantitative trait locus

ANOVA:

Analysis of variance

RLP:

Relative latency period

EA+:

Early aborted colonies, associated with plant cell necrosis

EA−:

Early aborted colonies, without plant cell necrosis

EST+:

Established colonies, associated with plant cell necrosis

EST−:

Established colonies, without plant cell necrosis

NEC:

All necrotic colonies

SIM:

Simple interval mapping

rMQM:

Restricted multiple QTL model

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Acknowledgments

This work was supported by Ministero dell’Università e della Ricerca (MiUR) of Italy special grant AGROGEN, by Spanish Comisión Interministerial de Ciencia y Tecnología (CICYT) grant PET2007-0492, and by COST860–SUSVAR. We are very grateful to Dr. Marion Roder, who kindly provided sequences of GWM microsatellite primer pairs not available in literature.

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Authors and Affiliations

  1. C.R.A. Cereal Research Centre-SS, 16 km 675, 71100, Foggia, Italy

    Daniela Marone, Giovanni Laidò, Maria A. Russo, Pina Ferragonio, Valentina Giovanniello, Elisabetta Mazzucotelli, Anna M. De Leonardis, Pasquale De Vita, Luigi Cattivelli & Anna M. Mastrangelo

  2. Institute Sustainable Agriculture, CSIC, Apdo. 4084, 14080, Cordoba, Spain

    Ana I. Del Olmo & Diego Rubiales

  3. CIFA Alameda del Obispo, IFAPA-CICE, Cordoba, Spain

    Josefina C. Sillero

  4. Department of Agricultural Botany, Kafr El-Sheikh University, Kafr El-Sheikh, 33516, Egypt

    Amero A. Emeran

  5. Department of Environmental and Agro-Forestry Biology and Chemistry, University of Bari, Via Amendola 165/A, 70126, Bari, Italy

    Antonio Blanco

  6. CRA Genomic Research Centre, Via S. Protaso 302, 29017, Fiorenzuola d’Arda, Italy

    Luigi Cattivelli

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  1. Daniela Marone
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  2. Ana I. Del Olmo
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  3. Giovanni Laidò
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Correspondence to Anna M. Mastrangelo.

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Daniela Marone and Ana I. Del Olmo contributed equally to the work.

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Marone, D., Del Olmo, A.I., Laidò, G. et al. Genetic analysis of durable resistance against leaf rust in durum wheat. Mol Breeding 24, 25–39 (2009). https://doi.org/10.1007/s11032-009-9268-9

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