Skip to main content
SpringerLink
Log in

Mapping of QTLs prolonging the latent period of Puccinia triticina infection in wheat

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Slow rusting is considered a crucial component of durable resistance to wheat leaf rust caused by Puccinia triticina and is often expressed in the form of a prolonged latent period. Selection for a longer latent period is considered an effective approach to developing wheat cultivars with improved durable resistance to leaf rust. A recombinant inbred line (RIL) population derived from CI 13227 (long latent period) × Suwon 92 (short latent period) was phenotyped for latent period in two greenhouse experiments in separate years, and amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers were analyzed in the same population. Among the RILs, the frequency distribution for latent period was continuous, and latent period was highly correlated between years (r=0.94, P<0.0001). A quantitative trait locus (QTL) prolonging the latent period of P. triticina, designated as QLrlp.osu-2DS, explained 42.8% and 54.5% of the phenotypic and genetic variance in the two experiments, respectively. QLrlp.osu-2DS was mapped on the distal region of chromosome 2DS. Two other QTLs for latent period, QLrlp.osu-2B and QLrlp.osu-7BL, were localized on chromosome 2B and the long arm of chromosome 7B, respectively. Multiple regression analysis showed that these three QTLs collectively explained 58.0% and 73.8% of the phenotypic and genetic variance over two experiments, respectively. Fourteen RILs that carried all three alleles for long latent period at three AFLP loci flanking QLrlp.osu-2DS, QLrlp.osu-2B, and QLrlp.osu-7BL had a mean latent period of 12.5 days, whereas 13 RILs without any long-latent-period alleles at the corresponding loci had a mean latent period of 7.4 days. Three SSR markers closely linked to these QTLs have potential to be applied in marker-assisted selection for prolonged latent period in wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bai GH, Kolb F, Shaner G, Domier L (1999) AFLP markers linked to one major QTL controlling scab resistance in wheat. Phytopathology 89:343–348

    CAS  Google Scholar 

  • Bjarko ME, Line RF (1988a) Quantitative determination of the gene action of leaf rust resistance in four cultivars of wheat, Triticum aestivum. Phytopathology 78:451–456

    Google Scholar 

  • Bjarko ME, Line RF (1988b) Heritability and number of genes controlling leaf rust resistance in four cultivars of wheat. Phytopathology 78:456–461

    Google Scholar 

  • Broers LHM, Jacobs T (1989) The inheritance of host plant effect on latency period of leaf rust in spring wheat. II. Number of segregation factors and evidence for transgressive segregation in F3 and F5 generation. In: Broers LHM, Jacobs T (eds) Histological, genetical and epidemiological studies on partial resistance in wheat to leaf rust. Ponson and Looijen, Wageningen, pp 95–105

    Google Scholar 

  • Caldwell RM, Roberts JJ, Eyal Z (1970) General resistance (“slow rusting”) to Puccinia recondita f. sp. tritici in winter and spring wheat (abstr.). Phytopathology 60:1287

    Google Scholar 

  • Clifford BC (1972) The histology of race non-specific resistance to Punccinia hordei in barley. In: Proc Eur Mediterr Cereal Rusts Conf, vol 1. Research Institute of Crop Production, University Press, Prague, pp 75–79

  • Das MK, Rajaram S, Mundt CC, Kronstad (1992) Inheritance of slow-rusting resistance to leaf rust in wheat. Crop Sci 32:1452–1456

    Google Scholar 

  • Das MK, Rajaram S, Mundt CC, Kronstad WE, Singh RP (1993) Associations and genetics of three components of slow rusting in leaf rust in wheat. Euphytica 68:99–109

    Article  Google Scholar 

  • Faris JD, Li WL, Liu DJ, Chen PD, Gill BS (1999) Candidate gene analysis of quantitative disease resistance genes. Theor Appl Genet 98:219–225

    Article  CAS  Google Scholar 

  • Heath MC (1977) A comparative study of non-host interactions with rust fungi. Physiol Plant Pathol 10:73–88

    Google Scholar 

  • Jacobs T, Broers LHM (1989) The inheritance of host plant effect on latency period of wheat leaf rust in spring wheat. I. Estimation of gene action and number of effective factors in F1, F2 and backcross generations. In: Broers LHM, Jacobs T (eds) Histological, genetical and epidemiological studies on partial resistance in wheat to leaf rust. Ponson and Looijen, Wageningen, pp 79–91

    Google Scholar 

  • Knott DR (1989) The wheat rusts: breeding for resistance. Springer, Berlin Heidelberg New York, pp 144–145

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Kuhn RC, Ohm HW, Shaner G (1978) Slow leaf rusting resistance in wheat against twenty-two isolates of Puccinia recondita. Phytopathology 68:651–656

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg I (1987) mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Lee TS, Shaner GE (1985a) Oligogenic inheritance of length of latent period in six slow leaf-rusting wheat cultivars. Phytopathology 75:643–647

    Google Scholar 

  • Lee TS, Shaner G (1985b) Transgressive segregation of length of latent period in crosses between slow leaf-rusting wheat cultivars. Phytopathology 75:643–647

    Google Scholar 

  • Messmer MM, Seyfarth S, Keller M, Schachermayr G, Winzeler M, Zanetti S, Feuillet C, Keller B (2000) Genetic analysis of durable leaf rust resistance in winter wheat. Theor Appl Genet 100:419–431

    Article  CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers lined to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    CAS  PubMed  Google Scholar 

  • Milus EA, Line RE (1980) Characterization of resistance to leaf rust in Pacific Northwest wheats. Phytopathology 76:93–96

    Google Scholar 

  • Neervoort WJ, Parlevliet JE (1978) Partial resistance of barley to leaf rust, Puccinia hordei. V. Analysis of the components of partial resistance in eight barley cultivars. Euphytica 27:33–39

    Article  Google Scholar 

  • Nelson JC (1997b) qgene: software for maker-based genomic analysis and breeding. Mol Breed 3:239–245

    Article  CAS  Google Scholar 

  • Nelson JC, Singh RP, Autrique E, Sorrells ME (1997a) Mapping genes conferring and suppressing leaf rust resistance in wheat. Crop Sci 37:1928–1935

    CAS  Google Scholar 

  • Ohm HW, Shaner GE (1976) Three components of slow leaf-rusting at different growth stages in wheat. Phytopathology 66:1356–1360

    Google Scholar 

  • Parlevliet JE (1979) Components of resistance that reduce the rate of epidemic development. Ann Rev Phytopathol 17:203–222

    Article  Google Scholar 

  • Parlevliet JE, Van Ommeren A (1975) Partial resistance of barley to leaf rust, Puccinia hordei. II. Relationship between field trials, micro-plot tests and latent period. Euphytica 24:293–303

    Article  Google Scholar 

  • Rubiales D, Niks RE (1995) Characterization of Lr34, a major gene conferring nonhypersensitive resistance to wheat leaf rust. Plant Dis 79:1208–1212

    Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosome location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    PubMed  Google Scholar 

  • Schnursch T, Pailard S, Schori A, Messmer M, Schachermayr G, Winzeler M, Keller B (2004) Dissection of quantitative and durable leaf rust resistance in Swiss winter wheat reveals a major resistance QTL in the Lr34 chromosomal region. Theor Appl Genet 108:477–484

    Article  PubMed  Google Scholar 

  • Shan X, Blake TK, Talbert LE (1999) Conversion of AFLP markers to sequence-specific PCR markers in barley and wheat. Theor Appl Genet 98:1072–1078

    Article  CAS  Google Scholar 

  • Shaner G, Finney RE (1980) New sources of slow leaf-rusting resistance in wheat. Phytopathology 70:1183–1186

    Google Scholar 

  • Shaner G, Buechley G, Nyquist WE (1997) Inheritance of latent period of Punccinia recondita in wheat. Crop Sci 37:748–756

    Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum). Theor Appl Genet (10.1007/s00122-004-1740-710.1007/s00122-004-1740-7)

  • Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93:881–890

    CAS  Google Scholar 

  • VanderGaag DJ, Jacobs T (1997) Inheritance of host plant effect on latent period of leaf rust in single-seed descent F8 lines. Euphytica 93:67–72

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frifiters A, Pot J, Peleman J, Kuiper M, Xabeaum M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    CAS  PubMed  Google Scholar 

  • William HM, Hoisington D, Singh RP, Gonzalez-Leon D (1997) Detection of quantitative trait loci associated with leaf rust resistance in bread wheat. Genome 40:253–260

    Article  Google Scholar 

  • William M, Singh RP, Huerta-Espino J, Ortiz Islas S, Hoisington D (2003) Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe resistance gene Yr29 in wheat. Phytopathology 93:153–159

    CAS  Google Scholar 

  • Zadoks JC (1971) Systems analysis and the dynamics of epidemics. Phytopathology 61:600–610

    Google Scholar 

Download references

Acknowledgements

We thank Perry Cregan and Qijian Song (Soybean Genomics and Improvement Laboratory, USDA, ARS, Beltsville Agricultural Research Center, Beltsville, Md., USA) for providing the BARC SSR primer sequences, Richard Ward (Department of Crop and Soil Science, Michigan State University, East Lansing, Mich., USA) and Bikram S. Gill (Department of Pathology, Kansas State University, Manhattan, Kan., USA) for providing mapping information for the BARC SSR markers, and Daryl Somers for providing primer and mapping information for the WMC SSR markers.

Author information

Authors and Affiliations

  1. Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

    X.-Y. Xu & B. F. Carver

  2. USDA-ARS-NPA-GMPRC Plant Science and Entomology Research Unit, Manhattan, KS, 66506, USA

    G.-H. Bai

  3. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA

    G. E. Shaner

  4. Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA

    R. M. Hunger

Authors
  1. X.-Y. Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G.-H. Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. F. Carver
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. E. Shaner
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. M. Hunger
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G.-H. Bai.

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, XY., Bai, GH., Carver, B.F. et al. Mapping of QTLs prolonging the latent period of Puccinia triticina infection in wheat. Theor Appl Genet 110, 244–251 (2005). https://doi.org/10.1007/s00122-004-1819-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-004-1819-1

Keywords

Search

Navigation