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Dissection of quantitative and durable leaf rust resistance in Swiss winter wheat reveals a major resistance QTL in the Lr34 chromosomal region

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Abstract

The Swiss winter bread wheat cv. ‘Forno’ has a highly effective, durable and quantitative leaf rust (Puccinia triticina Eriks.) resistance which is associated with leaf tip necrosis (LTN). We studied 240 single seed descent lines of an ‘Arina×Forno’ F5:7 population to identify and map quantitative trait loci (QTLs) for leaf rust resistance and LTN. Percentage of infected leaf area (%) and the response to infection (RI) were evaluated in seven field trials and were transformed to the area under the disease progress curves (AUDPC). Using composite interval mapping and LOD >4.4, we identified eight chromosomal regions specifically associated with resistance. The largest and most consistent leaf rust resistance locus was identified on the short arm of chromosome 7D (32.6% of variance explained for AUDPC_% and 42.6% for AUDPC_RI) together with the major QTL for LTN (R 2=55.6%) in the same chromosomal region as Lr34 (Xgwm295). A second major leaf rust resistance QTL (R 2=28% and 31.5%, respectively) was located on chromosome arm 1BS close to Xgwm604 and was not associated with LTN. Additional minor QTLs for LTN (2DL, 3DL, 4BS and 5AL) and leaf rust resistance were identified. These latter QTLs might correspond to the leaf rust resistance genes Lr2 or Lr22 (2DS) and Lr14a (7BL).

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Acknowledgements

We thank B. Senger, K. Hintermann-Stein and P. Streckeisen for their excellent technical assistance and R. Enderlin for conducting the field trials in Haag, Canton St. Gallen, Switzerland. We are thankful to Dr. C. Feuillet for critical reading of the manuscript and to Prof. Dr. H.F. Utz for providing the computer packages PLABSTAT and PLABQTL. This work was supported by the University of Zürich. The authors declare that the experiments conducted for this publication comply with the current laws of Switzerland.

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Authors and Affiliations

  1. Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, 8008, Zürich, Switzerland

    T. Schnurbusch & B. Keller

  2. Swiss Federal Research Station for Agroecology and Agriculture (FAL), Reckenholzstrasse 191, 8046, Zürich, Switzerland

    S. Paillard, G. Schachermayr & M. Winzeler

  3. Swiss Federal Research Station for Plant Production, Changins, Route de Duillier, P.O. Box 254, 1260 , Nyon 1, Switzerland

    A. Schori

  4. Pharmaceutical Institute, University of Basel, Benkenstrasse 254, 4108, Witterswil, Switzerland

    M. Messmer

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  1. T. Schnurbusch
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  2. S. Paillard
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  3. A. Schori
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  4. M. Messmer
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  5. G. Schachermayr
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  6. M. Winzeler
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  7. B. Keller
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Correspondence to B. Keller.

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Communicated by H.C. Becker

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Schnurbusch, T., Paillard, S., Schori, A. et al. Dissection of quantitative and durable leaf rust resistance in Swiss winter wheat reveals a major resistance QTL in the Lr34 chromosomal region. Theor Appl Genet 108, 477–484 (2004). https://doi.org/10.1007/s00122-003-1444-4

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