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FM reconstruction of non-uniformly sampled protein NMR data at higher dimensions and optimization by distillation

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Abstract

Non-uniform sampling (NUS) enables recording of multidimensional NMR data at resolutions matching the resolving power of modern instruments without using excessive measuring time. However, in order to obtain satisfying results, efficient reconstruction methods are needed. Here we describe an optimized version of the Forward Maximum entropy (FM) reconstruction method, which can reconstruct up to three indirect dimensions. For complex datasets, such as NOESY spectra, the performance of the procedure is enhanced by a distillation procedure that reduces artifacts stemming from intense peaks.

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Acknowledgment

This research was supported by the National Institutes of Health (grants GM 47467 and EB 002026). We thank Dr. Jeffrey Hoch for fruitful discussion on the topic of this manuscript and Mr. Gregory Heffron for assistance with the spectrometers.

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Authors and Affiliations

  1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA, 02115, USA

    Sven G. Hyberts, Dominique P. Frueh, Haribabu Arthanari & Gerhard Wagner

Authors
  1. Sven G. Hyberts
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  2. Dominique P. Frueh
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  3. Haribabu Arthanari
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  4. Gerhard Wagner
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Corresponding author

Correspondence to Gerhard Wagner.

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The FM reconstruction software is available upon request.

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Hyberts, S.G., Frueh, D.P., Arthanari, H. et al. FM reconstruction of non-uniformly sampled protein NMR data at higher dimensions and optimization by distillation. J Biomol NMR 45, 283–294 (2009). https://doi.org/10.1007/s10858-009-9368-1

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  • DOI: https://doi.org/10.1007/s10858-009-9368-1

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