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Application of nonlinear sampling schemes to COSY-type spectra

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Summary

Nonlinear sampling along the t1 dimension is applied to COSY-type spectra. The sine dependence of the time domain signals for the cross peaks is matched by a nonlinear sampling scheme that samples most densely around the maximum of the sine function. Data are processed by maximum entropy reconstruction, using a modified implementation of the ‘Cambridge’ algorithm of Skilling and Bryan. The procedure is demonstrated for P.E.COSY spectra recorded on a cyclic hexapeptide and on a 126-residue domain of the protein villin. The number of t1 values in the nonlinearly sampled experiments was reduced by a factor of four compared to linear sampling. The sensitivity and resolution of the resulting spectra are comparable to those achieved by conventional methods. The method described can thus significantly reduce the measuring time for COSY-type spectra.

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Abbreviations

COSY:

two-dimensional correlation spectroscopy

E.COSY:

exclusive COSY

P.E.COSY:

primitive E.COSY

TPPI:

time proportional phase incrementation

FID:

free induction decay

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Authors and Affiliations

  1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, 02115, Boston, MA, U.S.A.

    Peter Schmieder & Gerhard Wagner

  2. Rowland Institute for Science, 100 Edwin H. Land Boulevard, 02142, Cambridge, MA, U.S.A.

    Alan S. Stern & Jeffrey C. Hoch

Authors
  1. Peter Schmieder
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  2. Alan S. Stern
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  3. Gerhard Wagner
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  4. Jeffrey C. Hoch
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Schmieder, P., Stern, A.S., Wagner, G. et al. Application of nonlinear sampling schemes to COSY-type spectra. J Biomol NMR 3, 569–576 (1993). https://doi.org/10.1007/BF00174610

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  • DOI: https://doi.org/10.1007/BF00174610

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