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Insights into the role of seaweed Kappaphycus alvarezii sap towards phytohormone signalling and regulating defence responsive genes in Lycopersicon esculentum

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Abstract

Seaweeds, an integral part of marine coastal environment, are an important food, industrial raw materials and biostimulants for crop growth due to the presence of a number of plant growth stimulants. The sap of an economically important seaweed Kappaphycus alvarezii (K-sap) is gaining momentum for sustainable intensification of agricultural productivity. In the present study, the phytohormone levels and transcript regulation of defence-related genes were studied in tomato seedlings in response to K-sap application alone and in combination with the fungus Macrophomina phaseolina, the causal agent of charcoal rot in tomato. The application of K-sap alone and in combination with fungus M. phaseolina significantly increased the concentration of ABA, IAA, SA and zeatin hormones. The enhanced transcript of pathogenesis related genes (PR-1b1, PR-3 and PR-5) was observed in response to application of K-sap alone and in combination with fungus M. phaseolina in tomato. The transcription factor, Pti4 and mitogen-activated kinase pathway gene, MPK2 also showed transcript accumulation on combined treatment of Macrophomina and K-sap. This is the first report highlighting the differential regulation of defence-related genes in accordance with phytohormone levels on application of seaweed sap.

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Acknowledgments

CSIR-CSMCRI Communication No. (as provided by BDIM)—045/2015.

The authors are thankful to Dr. PK Ghosh for helpful discussion and Council of Scientific and Industrial Research (CSIR), New Delhi, India, for financial assistance. PA acknowledges the financial support from CSIR-Pool Scientist’s Scheme.

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Authors and Affiliations

  1. Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, 364 002, Gujarat, India

    Parinita Agarwal, Khantika Patel, Arup Ghosh & Pradeep K. Agarwal

  2. Analytical Division and Centralized Instrument Facility, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, 364 002, Gujarat, India

    Arun K. Das

  3. Academy of Scientific and Innovative Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, 364 002, Gujarat, India

    Arup Ghosh & Pradeep K. Agarwal

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  1. Parinita Agarwal
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  2. Khantika Patel
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Agarwal, P., Patel, K., Das, A.K. et al. Insights into the role of seaweed Kappaphycus alvarezii sap towards phytohormone signalling and regulating defence responsive genes in Lycopersicon esculentum . J Appl Phycol 28, 2529–2537 (2016). https://doi.org/10.1007/s10811-015-0784-1

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