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Polyamine catabolism influences antioxidative defense mechanism in shoots and roots of five wheat genotypes under high temperature stress

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Abstract

Effect of high temperature stress on polyamine catabolism and antioxidant enzyme activity in relation to glutathione, ascorbate and proline accumulation was studied in five wheat (Triticum aestivum L.) genotypes (differently susceptible to temperature stress). High temperature significantly increased the activities of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR) and glutathione S-transferase (GST) in shoots of all genotypes. Higher activities of GPX in C 306, C 273 and APX in PBW 550, PBW 343 and PBW 534 demonstrate their important role in scavenging H2O2. Conversely, high temperature stress led to a significant decline in SOD, CAT, APX and GPX activities of roots with a subsequent increase in diamine oxidase (DAO) and polyamine oxidase (PAO) activities especially in PBW 550 and PBW 343. The concentration of ascorbic acid declined with the imposition of heat stress, however, polyamines responded to high temperature stress by increasing spermidine and spermine levels and decreasing putrescine levels. After exposure to high temperature, proline accumulation was significantly decreased in roots and increased in shoots though maximum concentration was achieved in C 306 genotype. Apparently, the wheat seedlings respond to high temperature mediated increase in reactive oxygen species (ROS) production by altering antioxidative defense mechanism and polyamine catabolism though differentially in five wheat genotypes. Among five genotypes studied, C 306 and C 273 seem to be better protected against temperature stress. The results suggested that shoots were more resistant against the destructive effects of ROS as is indicated by low levels of thiobarbituric acid reactive substances under high temperature stress.

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Abbreviations

SOD:

Superoxide dismutase

CAT:

Catalase

GPX:

Guaiacol peroxidase

APX:

Ascorbate peroxidase

DHAR:

Dehydroascorbate reductase

GR:

Glutathione reductase

GST:

Glutathione S-transferase

DAO:

Diamine oxidase

PAO:

Polyamine oxidase

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

TBARS:

Thiobarbituric acid reactive substances

PA:

Polyamines

ROS:

Reactive oxygen species

GSH:

Reduced glutathione

GSSR:

Oxidized glutathione

NBT:

Nitro blue tetrazolium

AsA:

Reduced ascorbate

DHA:

Dehydroascorbate

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Acknowledgments

Funding from Department of Biotechnology, New Delhi for this project is duly acknowledged. Thanks are also due to Dr Navtej Singh Bains, Plant Breeder, PAU Ludhiana, Punjab for providing seeds. Authors also sincerely thank Dr S. S. Gosal, Additional Director of Research, PAU for coordinating research projects running parallel in other departments of the university.

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  1. Department of Biochemistry, Punjab Agricultural University, Ludhiana, Punjab, 141004, India

    Meenakshi Goyal & Bavita Asthir

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  1. Meenakshi Goyal
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  2. Bavita Asthir
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Correspondence to Bavita Asthir.

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Goyal, M., Asthir, B. Polyamine catabolism influences antioxidative defense mechanism in shoots and roots of five wheat genotypes under high temperature stress. Plant Growth Regul 60, 13–25 (2010). https://doi.org/10.1007/s10725-009-9414-8

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