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Polyamine content in Arabidopsis thaliana (L.) Heynh during recovery after low and high temperature treatments

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Abstract

Comparative studies on the effect of temperature treatment on the endogenous polyamine content in wild type and the ethylene insensitive mutant eti5 of Arabidopsis thaliana (L.) Heynh were performed. The levels of free and conjugated putrescine, spermidine and spermine were measured in rosette leaves of 38-day-old plants subjected to low and high temperature for 24 h in darkness. Data for fractions measured in treated wild type plants during recovery suggest that alterations in polyamine levels may be a consequence of the conversion of the supernatant-bound into free form and vice versa, while in treated eti5 plants de novo synthesis of spermidine and spermine could not be excluded. It was found that high temperature provoked more significant changes in polyamine levels than low temperature. The results suggest that the eti5 mutant showed a better ability to recover after the temperature treatments than wild type partly as a consequence of changes in polyamine content.

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Abbreviations

HT:

High temperature (38°C)

LT:

Low temperature (4°C)

PA:

Polyamine

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

TCA:

Trichloroacetic acid

Wt:

Wild type

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Acknowledgements

This work was supported by the INCO-COPERNICUS Programme ERBIC15CT960914.

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Authors and Affiliations

  1. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl.21, 1113, Sofia, Bulgaria

    Dessislava Todorova, Iskren Sergiev, Vera Alexieva & Emanuil Karanov

  2. Institute of Biological Sciences, University of Wales, Edward Llwyd Building, Aberystwyth, Ceredigion, SY23 3DA, UK

    Aileen Smith & Michael Hall

Authors
  1. Dessislava Todorova
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  2. Iskren Sergiev
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  3. Vera Alexieva
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  4. Emanuil Karanov
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  5. Aileen Smith
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  6. Michael Hall
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Correspondence to Dessislava Todorova.

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Todorova, D., Sergiev, I., Alexieva, V. et al. Polyamine content in Arabidopsis thaliana (L.) Heynh during recovery after low and high temperature treatments. Plant Growth Regul 51, 185–191 (2007). https://doi.org/10.1007/s10725-006-9143-1

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  • DOI: https://doi.org/10.1007/s10725-006-9143-1

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