Abstract
The increasing severity and prevalence of powdery mildew aided by extensive use of semi-dwarf cultivars and high levels of nitrogenous fertilizers are causing significant yield losses in wheat. Resistant cultivars are the most cost-effective and environmentally friendly approach to manage the disease. The objective of this study was to identify quantitative trait loci (QTL) for powdery mildew resistance in a doubled haploid (DH) population from a cross between leading Chinese cultivars, Yangmai 16 and Zhongmai 895. A high-density genetic map comprising of 14,480 non-redundant markers (equal to 148,179 SNPs) in 21 wheat chromosomes was constructed by genotyping the population with the Wheat 660 K SNP array. The DH population was phenotyped for powdery mildew resistance at the adult plant stage in multiple field trials, including four environments in the 2016–2017 cropping season and two environments in 2017–2018. Composite interval mapping detected six stable QTL explaining 3.8–23.6% of the phenotypic variance across environments. QPmyz.caas-5DS, QPmyz.caas-6BL and QPmyz.caas-7BS, are probably new QTL for powdery mildew resistance. One SNP marker closely linked to QPmyz.caas-6BL, the most stable QTL, was converted into a Kompetitive Allele-Specific PCR marker (K_AX-94973433) and validated on 103 commercial wheat cultivars. Significantly lower maximum disease severities of cultivars with the resistance-associated allele than those with the susceptibility-associated allele at QPmyz.caas-6BL in some environments indicated partial effectiveness of the marker. The novel QTL and their closely linked markers identified in the present study should facilitate development of cultivars with improved powdery mildew resistance.
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Acknowledgements
The authors are grateful to Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, for critical review of this manuscript. This study was supported by the National Key R&D Program of China (2016YFE0108600 and 2016YFD0101802) and CAAS Science and Technology Innovation Program.
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Xiaoting Xu performed the experimental work and data analysis, and wrote the paper. Zhanwang Zhu, Aolin Jia, Yelun Zhang, Chao Fu and Xianchun Xia participated in field trials. Fengju Wang constructed the DH population. Jinping Wang contributed to genotyping the DH lines. Zhanwang Zhu and Luping Fu helped with genetic map construction. Guihua Bai helped with the genetic map construction and KASP development. Yuanfeng Hao and Zhonghu He designed the experiment. Yuanfeng Hao, Zhonghu He and Xianchun Xia assisted in writing and revising the manuscript. All authors read and approved the final manuscript.
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Xu, X., Zhu, Z., Jia, A. et al. Mapping of QTL for partial resistance to powdery mildew in two Chinese common wheat cultivars. Euphytica 216, 3 (2020). https://doi.org/10.1007/s10681-019-2537-8
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DOI: https://doi.org/10.1007/s10681-019-2537-8