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QTL analysis of durable stripe rust resistance in the North American winter wheat cultivar Skiles

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This study determined the effects of growth stage and temperature on expression of high-temperature adult-plant resistance to stripe rust, mapped six QTL for durable resistance in winter wheat Skiles using a doubled haploid population, and selected breeding lines with different combinations of the QTL using marker-assisted selection.

Abstract

The winter wheat cultivar Skiles has a high level of high-temperature adult-plant (HTAP) resistance to stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). The Skiles HTAP resistance was highly effective at the adult-plant stage even under low temperatures, but high temperatures induced earlier expression and increased levels of resistance. To map resistance genes, Skiles was crossed with the susceptible cultivar Avocet S and a doubled haploid (DH) population was developed. The DH population was tested in fields at Pullman, WA, in 2016, 2017 and 2018, Mount Vernon, WA, in 2017 and 2018 under natural infection, and an environmentally controlled greenhouse at the adult-plant stage with the currently predominant race PSTv-37. The population was genotyped using the 90 K Illumina iSelect wheat SNP chip and selected SSR markers on specific chromosomes. In total, 2526 polymorphic markers were used for QTL mapping and six QTL were detected. Two of the six QTL had major effects across all environments, with one mapped on chromosome 3BS, explaining up to 28.2% of the phenotypic variation and the other on chromosome 4BL, explaining up to 41.8%. Minor QTL were mapped on chromosomes 1BL, 5AL, 6B and 7DL. Genotyping 140 wheat cultivars from the US Pacific Northwest revealed high polymorphism of markers for five of the QTL, and five highly resistant lines with the five QTL were selected from Skiles-derived breeding lines using the markers. This study demonstrated that multiple QTL with mostly additive effects contributed to the high-level HTAP resistance in Skiles.

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Acknowledgments

This research was supported by the U.S. Department of Agriculture, Agricultural Research Service (Project No. 2090-22000-018-00D), Vogel Foundation (Project No. 13Z-3061-6665), Washington Grain Commission (Project No. 13C-3061-5665) and Washington State University, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, HATCH Project Number WNP00461, Washington State University, Pullman, WA 99164-6430, USA. The authors would like to thank Anna Kondratiuk for technical assistance in doubled haploid line development, Sheri Rynearson for assistance with KASP marker development, Shiaoman Chao for SNP genotyping, and Kent Evans and Jason Sprott for field preparation and weed control. The China Scholarship Council scholarship to Lu Liu is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    L. Liu, C. Y. Yuan, M. N. Wang, D. R. See & X. M. Chen

  2. College of Life Sciences, Luoyang Normal University, Luoyang, 471934, Henan, People’s Republic of China

    C. Y. Yuan

  3. USDA-ARS, Wheat Health, Genetics and Quality Research Unit, Pullman, WA, 99164-6430, USA

    D. R. See & X. M. Chen

  4. Department of Crop and Soil Sciences, Oregon State University, Corvallis, OR, 97331-3002, USA

    R. S. Zemetra

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  1. L. Liu
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Correspondence to X. M. Chen.

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All experiments and data analyses were conducted in Pullman and Mount Vernon, Washington, and the 90 K SNP genotype was done at Fargo, North Dakota. All authors contributed to the study and approved the submitted version. The manuscript has not been submitted to any other journal.

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Fig. S1

Reactions of Skiles and AvS to Puccinia striiformis f. sp. tritici in fields (PPTX 573 kb)

Supplementary material 2 (XLSX 88 kb)

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Supplementary material 5 (XLSX 25 kb)

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Liu, L., Yuan, C.Y., Wang, M.N. et al. QTL analysis of durable stripe rust resistance in the North American winter wheat cultivar Skiles. Theor Appl Genet 132, 1677–1691 (2019). https://doi.org/10.1007/s00122-019-03307-2

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