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Identification of wheat-Secale africanum chromosome 2Rafr introgression lines with novel disease resistance and agronomic characteristics

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Abstract

The wild species, Secale africanum Stapf., serves as a valuable germplasm resource for increasing the diversity of cultivated rye (Secale cereale L.) and providing novel genes for wheat improvement. The objective of this study was to identify new wheat-S. africanum chromosome 2Rafr derivatives by molecular markers and in situ hybridization, and to test the effects of centric translocations of S. africanum chromosome 2Rafr on disease resistance and agronomic performance of wheat. The T2RafrS.2DL and T2DS.2RafrL translocations, and other S. africanum chromosome introgressions such as a 2Rafr substitution as well as monotelo-2RafrS and 2RafrL addition lines, were identified by the genomic in situ hybridization and sequential fluorescence in situ hybridization (FISH). Twenty-three molecular markers were localized on chromosome 2Rafr arms which will facilitate future identification of their introgressions into wheat. A comparison of FISH patterns and the molecular marker distribution between the S. africanum chromosome 2Rafr and cultivated rye chromosome 2R indicates differentiation between the wild and cultivated Secale genomes. Tests of disease resistance and agronomic performance of wheat-S. africanum chromosome 2Rafr derivatives indicated that chromosome 2RafrL carried gene(s) for dwarfing and stripe rust resistance, while the 2RafrS translocation line was the most favorable for agronomic performance when compared with the substitution and 2RafrL translocation lines. These results encourage the continued use of novel wheat-S. africanum 2Rafr derivative lines in wheat improvement.

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Acknowledgments

We particularly thank Dr. I. Dundas at University of Adelaide, Australia for reviewing the manuscript. We are thankful to the National Natural Science Foundation of China (No. 30871518, 31171542 and 31101143), Fundamental Research Funds for the Central Universities of China (ZYGX2010J099, ZYGX2011J101) for their financial support.

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Authors and Affiliations

  1. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China

    Meng-Ping Lei, Guang-Rong Li, Li Zhou, Cheng-Hui Li, Cheng Liu & Zu-Jun Yang

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  1. Meng-Ping Lei
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  2. Guang-Rong Li
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  3. Li Zhou
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  4. Cheng-Hui Li
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  5. Cheng Liu
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Correspondence to Zu-Jun Yang.

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M. P. Lei and G. R. Li contributed equally to this study.

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Lei, MP., Li, GR., Zhou, L. et al. Identification of wheat-Secale africanum chromosome 2Rafr introgression lines with novel disease resistance and agronomic characteristics. Euphytica 194, 197–205 (2013). https://doi.org/10.1007/s10681-013-0913-3

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