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Genome relationships in the genus Dasypyrum: evidence from molecular phylogenetic analysis and in situ hybridization

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Abstract

The genus Dasypyrum (or Haynaldia) consists of two species, D. villosum and D. breviaristatum. However, the genomic relationships between these two species remain unclear. The objective of this study was to provide molecular phylogenic and cytological evidence on the evolutionary relationships of the genus Dasypyrum. Sequences of Chloroplast DNA (cpDNA) and α-gliadin genes both support the hypothesis that diploid D. breviaristatum is the progenitor of tetraploid D. breviaristatum, and the diploid D. villosum and D. breviaristatum evolved parallel from an ancestral species. Genomic and fluorescence in situ hybridization using ribosomal DNA and rye repetitive DNA sequence as probes also indicated that tetraploid D. breviaristatum originated from diploid D. breviaristatum.

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Acknowledgments

We thank the National Natural Science Foundation of China (No. 30671288, 30871518) and Chinese Ministry of Education (NCET-06-0810) for financial support. We particularly thank Dr. Shoji Ohta, W. Jon Raupp and the NPGS for kindly providing seeds. This research was also supported by grants from the Kansas Wheat Commission and the Kansas Crop Improvement Association. This paper is contribution no. 10-267-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, Kansas, 66506-5502.

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Authors and Affiliations

  1. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, People’s Republic of China

    Cheng Liu, Guang-Rong Li, Ju-Qing Jia & Zu-Jun Yang

  2. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA

    Cheng Liu, Sunish Kumar Sehgal, Bernd Friebe & Bikram Gill

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  1. Cheng Liu
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Correspondence to Bernd Friebe.

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C. Liu and G.-R. Li contributed equally to this paper.

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Liu, C., Li, GR., Sehgal, S.K. et al. Genome relationships in the genus Dasypyrum: evidence from molecular phylogenetic analysis and in situ hybridization. Plant Syst Evol 288, 149–156 (2010). https://doi.org/10.1007/s00606-010-0319-9

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  • DOI: https://doi.org/10.1007/s00606-010-0319-9

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