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Identification and development of diagnostic markers for a powdery mildew resistance gene on chromosome 2R of Chinese rye cultivar Jingzhouheimai

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An Erratum to this article was published on 08 April 2011

Abstract

Chinese rye cultivar Jingzhouheimai (Secale cereale L.) shows a high level of resistance to powdery mildew. Identification, location, and mapping of the resistance gene would be helpful for developing a highly resistant germplasm or cultivar in wheat. Using sequential C-banding, GISH, and marker analysis, an addition chromosome with powdery mildew resistance was identified in a line derived from a cross between Chinese wheat landrace Huixianhong and rye cultivar Jingzhouheimai. The line, designated H-J DA2RDS1R(1D), had 44 chromosomes including two pairs of rye chromosomes, 1R and 2R, and lacked a pair of wheat chromosomes 1D, that is, it is a double disomic addition disomic substitution line. According to its reaction to different isolates of the powdery mildew pathogen, the resistance gene in H-J DA2RDS1R(1D) differed from the Pm8 and Pm7 genes located earlier on rye chromosomes 1R and 2R, respectively. In order to determine the location of the resistance gene, line H-J DA2RDS1R(1D) was crossed with wheat landrace Huixianhong and the F2 population and corresponding F2:3 families were tested for disease reaction and assessed with molecular markers. The results showed that a resistance gene, designated PmJZHM2RL, is located in rye chromosome arm 2RL.

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Acknowledgments

The authors thank Dr. Yilin Zhou, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China, for conducting tests of seedling reactions to powdery mildew, Dr. Peter Wehling, Institute of Agricultural Crops, Groß Lüsewitz, Germany, and Dr. Michael Francki, Department of Agriculture and Food, Murdoch University, Perth, Australia, for kindly providing primers. This work was supported by the National Natural Science Foundation of China (No: 30500048, 30770222), NAU-CAU Youth Sci-Tech Innovation Fund (No: NC2008001), the 973 project (2009CB118304) and the 111 Project (No: B08025).

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  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China

    L. F. Zhuang, L. Sun, A. X. Li, T. T. Chen & Z. J. Qi

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  1. L. F. Zhuang
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  2. L. Sun
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  3. A. X. Li
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Correspondence to Z. J. Qi.

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L. F. Zhuang and L. Sun these two authors contributed equally.

An erratum to this article can be found at http://dx.doi.org/10.1007/s11032-011-9570-1

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Zhuang, L.F., Sun, L., Li, A.X. et al. Identification and development of diagnostic markers for a powdery mildew resistance gene on chromosome 2R of Chinese rye cultivar Jingzhouheimai. Mol Breeding 27, 455–465 (2011). https://doi.org/10.1007/s11032-010-9443-z

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