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Improved xylose fermentation of Kluyveromyces marxianus at elevated temperature through construction of a xylose isomerase pathway

  • Bioenergy/Biofuels/Biochemicals
  • Published:
Journal of Industrial Microbiology & Biotechnology

An Erratum to this article was published on 04 June 2013

Abstract

To improve the xylose fermentation ability of Kluyveromyces marxianus, a xylose assimilation pathway through xylose isomerase was constructed. The genes encoding xylose reductase (KmXyl1) and xylitol dehydrogenase (KmXyl2) were disrupted in K. marxianus YHJ010 and the resultant strain was named YRL002. A codon-optimized xylose isomerase gene from Orpinomyces was transformed into K. marxianus YRL002 and expressed under GAPDH promoter. The transformant was adapted in the SD medium containing 1 % casamino acid with 2 % xylose as sole carbon source. After 32 times of trans-inoculation, a strain named YRL005, which can grow at a specific growth rate of 0.137/h with xylose as carbon source, was obtained. K. marxianus YRL005 could ferment 30.15 g/l of xylose and produce 11.52 g/l ethanol with a yield of 0.38 g/g, production rate of 0.069 g/l/h at 42 °C, and also could ferment 16.60 g/l xylose to produce 5.21 g/l ethanol with a yield of 0.31 g/g, and production rate of 0.054 g/l h at 45 °C. Co-fermentation with 2 % glucose could not improve the amount and yield of ethanol fermented from xylose obviously, but it could improve the production rate. Furthermore, K. marxianus YRL005 can ferment with the corn cob hydrolysate, which contained 20.04 g/l xylose to produce 8.25 g/l ethanol. It is a good platform to construct thermo-tolerant xylose fermentation yeast.

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Acknowledgments

We thank Professor Tamaki Hisanori from Kagoshima University and Kumagai Hidehiko from Ishikawa Prefectural University for providing us K. marxianus YHJ 010 and plasmids. This work was supported by a grant-in-aid from the National High Technology Research and Development Program (2012AA02A708), the National Basic Research Program of China (2011CBA00801), the National Natural Science Foundation of China (31070028), and the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry (WF2070000010).

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Authors and Affiliations

  1. School of Life Sciences, University of Science and Technology of China, Hefei, People’s Republic of China

    Rongliang Wang, Lulu Li, Biao Zhang, Xiaolian Gao, Dongmei Wang & Jiong Hong

  2. Hefei National Laboratory for Physical Science at the Microscale, Hefei, 230026, Anhui, People’s Republic of China

    Rongliang Wang, Lulu Li, Biao Zhang, Xiaolian Gao, Dongmei Wang & Jiong Hong

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  1. Rongliang Wang
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  2. Lulu Li
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Correspondence to Dongmei Wang or Jiong Hong.

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Wang, R., Li, L., Zhang, B. et al. Improved xylose fermentation of Kluyveromyces marxianus at elevated temperature through construction of a xylose isomerase pathway. J Ind Microbiol Biotechnol 40, 841–854 (2013). https://doi.org/10.1007/s10295-013-1282-6

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