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Increased xylose reductase activity in the xylose-fermenting yeastPichia stipitis by overexpression ofXYL1

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Abstract

ThePichia stipitis xylose reductase gene (XYL1) was inserted into an autonomous plasmid thatP. stipitis maintains in multicopy. The plasmid pXOR with theXYL1 insert or a control plasmid pJM6 withoutXYL1 was introduced intoP. stipitis. When grown on xylose under aerobic conditions, the strain with pXOR had up to 1.8-fold higher xylose reductase (XOR) activity than the control strain. Oxygen limitation led to higher XOR activity in both experimental and control strains grown on xylose. However, the XOR activities of the two strains grown on xylose were similar under oxygen limitation. When grown on glucose under aerobic or oxygen-limited conditions, the experimental strain had XOR activity up to 10 times higher than that of the control strain. Ethanol production was not improved, but rather it decreased with the introduction of pXOR compared to the control, and this was attributed to nonspecific effects of the plasmid.

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Authors and Affiliations

  1. Department of Biochemistry, University of Wisconsin, Madison, WI

    Kristine M. Dahn & Paul E. Pittman

  2. Institute for Microbial and Biochemical Technology, Forest Products Laboratory, USDA Forest Service, One Gifford Pinchot Drive, 53705, Madison, WI

    Kristine M. Dahn, Brian P. Davis & Thomas W. Jeffries

  3. Department of Bacteriology, University of Wisconsin, 53706, Madison, WI

    Brian P. Davis & Thomas W. Jeffries

  4. J. Whittier Biologies Inc., Madison, WI

    William R. Kenealy

Authors
  1. Kristine M. Dahn
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  2. Brian P. Davis
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  3. Paul E. Pittman
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  4. William R. Kenealy
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  5. Thomas W. Jeffries
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Dahn, K.M., Davis, B.P., Pittman, P.E. et al. Increased xylose reductase activity in the xylose-fermenting yeastPichia stipitis by overexpression ofXYL1 . Appl Biochem Biotechnol 57, 267–276 (1996). https://doi.org/10.1007/BF02941707

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