Abstract
L-valine is an essential branched-chain amino acid that cannot be synthesized by the human body and has a wide range of applications in food, medicine and feed. Market demand has stimulated people’s interest in the industrial production of L-valine. At present, the mutagenized or engineered Corynebacterium glutamicum is an effective microbial cell factory for producing L-valine. Because the biosynthetic pathway and metabolic network of L-valine are intricate and strictly regulated by a variety of key enzymes and genes, highly targeted metabolic engineering can no longer meet the demand for efficient biosynthesis of L-valine. In recent years, the development of omics technology has promoted the upgrading of traditional metabolic engineering to systematic metabolic engineering. This whole-cell-scale transformation strategy has become a productive method for developing L-valine producing strains. This review provides an overview of the biosynthesis and regulation mechanism of L-valine, and summarizes the current metabolic engineering techniques and strategies for constructing L-valine high-producing strains. Finally, the opinion of constructing a cell factory for efficiently biosynthesizing L-valine was proposed.
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (No. 31601459), the Natural Science Foundation of Jiangsu Province (No. BK20150149), China Postdoctoral Science Foundation Grant (No. 2016M590410) and Fundamental Research Funds for the Central Universities (No. JUSRP115A19).
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JL: Propose ideas, literature search, data analysis, visualization, writing—original draft; JX: Writing—review and editing; BW: investigation; ZR: supervision; WZ: Writing—review and editing, supervision.
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Liu, J., Xu, JZ., Wang, B. et al. L-valine production in Corynebacterium glutamicum based on systematic metabolic engineering: progress and prospects. Amino Acids 53, 1301–1312 (2021). https://doi.org/10.1007/s00726-021-03066-9
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DOI: https://doi.org/10.1007/s00726-021-03066-9