Skip to main content
SpringerLink
Log in

A boronic acid based glucose assay based on the suppression of the inner filter effect of gold nanoparticles on the orange fluorescence of graphene oxide quantum dots

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

The authors describe a non-enzymatioc glucose assay that has three features: (a) The use of a boronic acid as the recognition element; (b) the aggregation of gold nanoparticles (AuNPs), and (c) the use of graphene oxide quantum dots (GOQD) whose fluorescence matches the absorption of the AuNPs and thereby results in an inner filter effect (IFE). The GOQD display an orange colored fluorescence that is filtered off by the AuNPs due to an IFE. In the presence of 3-aminobenzeneboronic acid, glucose is bound to form a cationic species that causes the aggregation of AuNPs to form large assemblies. This is accompanied by a color change from red to blue, and the IFE no longer does occur so that the orange fluorescence of the GOQD pops up again. These findings were used to design an assay witha linear response in the  2.5 to 75 μmol L−1 glucose concentration range, with a detection limit of 0.65 μmol L−1. The method was applied to the determination of glucose in spiked diluted serum and gave satisfactory results.

Schematic of the detection of glucose based on inner filter effect of dispersed AuNPs on the orange fluorescence of graphene oxide quantum dots.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Yang J, Cho M, Lee Y (2016) Synthesis of hierarchical Ni(OH)(2) hollow nanorod via chemical bath deposition and its glucose sensing performance. Sensors Actuators B Chem 222:674–681

    Article  CAS  Google Scholar 

  2. Yu Z, Li H, Zhang X, Liu N, Tan W, Zhang X, Zhang L (2016) Facile synthesis of NiCo2O4@polyaniline core-shell nanocomposite for sensitive determination of glucose. Biosens Bioelectron 75:161–165

    Article  CAS  Google Scholar 

  3. Chen J, Ge J, Zhang L, Li Z, Qu L (2016) Poly(styrene sulfonate) and Pt bifunctionalized graphene nanosheets as an artificial enzyme to construct a colorimetric chemosensor for highly sensitive glucose detection. Sensors Actuators B Chem 233:438–444

    Article  Google Scholar 

  4. Steiner M-S, Duerkop A, Wolfbeis OS (2011) Optical methods for sensing glucose. Chem Soc Rev 40(9):4805–4839

    Article  CAS  Google Scholar 

  5. Fan Y, Tan X, Liu X, Ou X, Chen S, Wei S (2015) A novel non-enzymatic electrochemiluminescence sensor for the detection of glucose based on the competitive reaction between glucose and phenoxy dextran for concanavalin a binding sites. Electrochim Acta 180:471–478

    Article  CAS  Google Scholar 

  6. Gao ZF, Chen DM, Lei JL, Luo HQ, Li NB (2015) A regenerated electrochemical biosensor for label-free detection of glucose and urea based on conformational switch of i-motif oligonucleotide probe. Anal Chim Acta 897:10–16

    Article  CAS  Google Scholar 

  7. Fang X, Wu X-M, Hu X-L, Li Z-J, Wang G-L (2016) Native carbon nanodots as a fluorescent probe for assays based on the use of glucose oxidase or horseradish peroxidase. Microchim Acta 183(10):2761–2770

    Article  CAS  Google Scholar 

  8. Durán GM, Benavidez TE, Ríos Á, García CD (2016) Quantum dot-modified paper-based assay for glucose screening. Microchim Acta 183(2):611–616

    Article  Google Scholar 

  9. Sodzel D, Khranovskyy V, Beni V, Turner APF, Viter R, Eriksson MO, Holtz P-O, Janot J-M, Bechelany M, Balme S, Smyntyna V, Kolesneva E, Dubovskaya L, Volotovski I, Ubelis A, Yakimova R (2015) Continuous sensing of hydrogen peroxide and glucose via quenching of the UV and visible luminescence of ZnO nanoparticles. Microchim Acta 182(9):1819–1826

    Article  CAS  Google Scholar 

  10. Ding C, Yan Y, Xiang D, Zhang C, Xian Y (2016) Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay. Microchim Acta 183(2):625–631

    Article  CAS  Google Scholar 

  11. Çiftçi H, Alver E, Çelik F, Metin AÜ, Tamer U (2016) Non-enzymatic sensing of glucose using a glassy carbon electrode modified with gold nanoparticles coated with polyethyleneimine and 3-aminophenylboronic acid. Microchim Acta 183(4):1479–1486

    Article  Google Scholar 

  12. Tan L, Chen K, Huang C, Peng R, Luo X, Yang R, Cheng Y, Tang Y (2015) A fluorescent turn-on detection scheme for α-fetoprotein using quantum dots placed in a boronate-modified molecularly imprinted polymer with high affinity for glycoproteins. Microchim Acta 182(15):2615–2622

    Article  CAS  Google Scholar 

  13. Adolfsson KH, Hassanzadeh S, Hakkarainen M (2015) Valorization of cellulose and waste paper to graphene oxide quantum dots. RSC Adv 5(34):26550–26558

    Article  CAS  Google Scholar 

  14. He Y, Sun J, Feng D, Chen H, Gao F, Wang L (2015) Graphene quantum dots: highly active bifunctional nanoprobes for nonenzymatic photoluminescence detection of hydroquinone. Biosens Bioelectron 74:418–422

    Article  CAS  Google Scholar 

  15. Du X, Jiang D, Liu Q, Zhu G, Mao H, Wang K (2015) Fabrication of graphene oxide decorated with nitrogen-doped graphene quantum dots and its enhanced electrochemiluminescence for ultrasensitive detection of pentachlorophenol. Analyst 140(4):1253–1259.S

    Article  CAS  Google Scholar 

  16. Benitez-Martinez S, Valcarcel M (2015) Fluorescent determination of graphene quantum dots in water samples. Anal Chim Acta 896:78–84

    Article  CAS  Google Scholar 

  17. Zhang L, Peng D, Liang R-P, Qiu J-D (2015) Graphene quantum dots assembled with Metalloporphyrins for "turn on" sensing of hydrogen peroxide and glucose. Chem Eur J 21(26):9343–9348

    Article  CAS  Google Scholar 

  18. Pei H, Zhu S, Yang M, Kong R, Zheng Y, Qu F (2015) Graphene oxide quantum dots@silver core-shell nanocrystals as turn-on fluorescent nanoprobe for ultrasensitive detection of prostate specific antigen. Biosens Bioelectron 74:909–914

    Article  CAS  Google Scholar 

  19. Abnous K, Danesh NM, Ramezani M, Emrani AS, Taghdisi SM (2016) A novel colorimetric sandwich aptasensor based on an indirect competitive enzyme-free method for ultrasensitive detection of chloramphenicol. Biosens Bioelectron 78:80–86

    Article  CAS  Google Scholar 

  20. Xianyu Y, Xie Y, Wang N, Wang Z, Jiang X (2015) A dispersion-dominated chromogenic strategy for colorimetric sensing of glutathione at the Nanomolar level using gold nanoparticles. Small 11(41):5510–5514

    Article  CAS  Google Scholar 

  21. Xia N, Zhou B, Huang N, Jiang M, Zhang J, Liu L (2016) Visual and fluorescent assays for selective detection of beta-amyloid oligomers based on the inner filter effect of gold nanoparticles on the fluorescence of CdTe quantum dots. Biosens Bioelectron 85:625–632

    Article  CAS  Google Scholar 

  22. Liu Z, Liu H, Wang L, Su X (2016) A label-free fluorescence biosensor for highly sensitive detection of lectin based on carboxymethyl chitosan-quantum dots and gold nanoparticles. Anal Chim Acta 932:88–97

    Article  CAS  Google Scholar 

  23. Zhao W, Brook MA, Li Y (2008) Design of Gold Nanoparticle-Based Colorimetric Biosensing Assays. Chembiochem 9(15):2363–2371

    Article  CAS  Google Scholar 

  24. Lou J, Liu S, Tu W, Dai Z (2015) Graphene Quantums dots combined with endonuclease cleavage and bidentate chelation for highly sensitive Electrochemiluminescent DNA Biosensing. Anal Chem 87(2):1145–1151

    Article  CAS  Google Scholar 

  25. Song L, Shi J, Lu J, Lu C (2015) Structure observation of graphene quantum dots by single-layered formation in layered confinement space. Chem Sci 6(8):4846–4850

    Article  CAS  Google Scholar 

  26. Vasu KS, Sridevi S, Sampath S, Sood AK (2015) Non-enzymatic electronic detection of glucose using aminophenylboronic acid functionalized reduced graphene oxide. Sensors Actuators B Chem 221:1209–1214

    Article  CAS  Google Scholar 

  27. Qian S, Liang Y, Ma J, Zhang Y, Zhao J, Peng W (2015) Boronic acid modified fiber optic SPR sensor and its application in saccharide detection. Sensors Actuators B Chem 220:1217–1223

    Article  CAS  Google Scholar 

  28. Cayuela A, Soriano ML, Valcarcel M (2015) Reusable sensor based on functionalized carbon dots for the detection of silver nanoparticles in cosmetics via inner filter effect. Anal Chim Acta 872:70–76

    Article  CAS  Google Scholar 

  29. Wu Q, Wang X, Liao C, Wei Q, Wang Q (2015) Microgel coating of magnetic nanoparticles via bienzyme-mediated free-radical polymerization for colorimetric detection of glucose. Nanoscale 7(40):16578–16582

    Article  CAS  Google Scholar 

  30. Xiong Y, Zhang Y, Rong P, Yang J, Wang W, Liu D (2015) A high-throughput colorimetric assay for glucose detection based on glucose oxidase-catalyzed enlargement of gold nanoparticles. Nanoscale 7(38):15584–15588

    Article  CAS  Google Scholar 

  31. Wang Y, Zhang S, Bai W, Zheng J (2016) Layer-by-layer assembly of copper nanoparticles and manganese dioxide-multiwalled carbon nanotubes film: a new nonenzymatic electrochemical sensor for glucose. Talanta 149:211–216

    Article  CAS  Google Scholar 

  32. Fang L, Liu B, Liu L, Li Y, Huang K, Zhang Q (2016) Direct electrochemistry of glucose oxidase immobilized on Au nanoparticles-functionalized 3D hierarchically ZnO nanostructures and its application to bioelectrochemical glucose sensor. Sensors Actuators B Chem 222:1096–1102

    Article  CAS  Google Scholar 

  33. Qu F, Guo X, Liu D, Chen G, You J (2016) Dual-emission carbon nanodots as a ratiometric nanosensor for the detection of glucose and glucose oxidase. Sensors Actuators B Chem 233:320–327

    Article  CAS  Google Scholar 

  34. Lerner MB, Kybert N, Mendoza R, Villechenon R, Bonilla Lopez MA, Charlie Johnson AT (2013) Scalable, non-invasive glucose sensor based on boronic acid functionalized carbon nanotube transistors. Appl Phys Lett 102(18):183113

    Article  Google Scholar 

  35. Zhao YQ, Luo HQ, Li NB (2009) Electrochemical characterization of in situ functionalized gold p-aminothiophenol self-assembled monolayer with 4-formylphenylboronic acid for recognition of sugars. Sensors Actuators B Chem 137(2):722–726

    Article  CAS  Google Scholar 

  36. Badhulika S, Tlili C, Mulchandani A (2014) Poly(3-aminophenylboronic acid)-functionalized carbon nanotubes-based chemiresistive sensors for detection of sugars. Analyst 139(12):3077–3082

    Article  CAS  Google Scholar 

  37. Tang X, Zhang B, Xiao C, Zhou H, Wang X, He D (2016) Carbon nanotube template synthesis of hierarchical NiCoO2 composite for non-enzyme glucose detection. Sensors Actuators B Chem 222:232–239

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Nos. 21075050 and 21275063), the Science and Technology Development project of Jilin province, China (No. 20150204010GX).

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China

    Weidan Na, Hua Liu, Mingyue Wang & Xingguang Su

Authors
  1. Weidan Na
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hua Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mingyue Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xingguang Su
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xingguang Su.

Ethics declarations

The author(s) declare that they have no competing interests.

Electronic supplementary material

ESM 1

(DOC 6411 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Na, W., Liu, H., Wang, M. et al. A boronic acid based glucose assay based on the suppression of the inner filter effect of gold nanoparticles on the orange fluorescence of graphene oxide quantum dots. Microchim Acta 184, 1463–1470 (2017). https://doi.org/10.1007/s00604-017-2090-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-017-2090-x

Keywords

Search

Navigation