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Continuous sensing of hydrogen peroxide and glucose via quenching of the UV and visible luminescence of ZnO nanoparticles

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Abstract

We report on an indirect optical method for the determination of glucose via the detection of hydrogen peroxide (H2O2) that is generated during the glucose oxidase (GOx) catalyzed oxidation of glucose. It is based on the finding that the ultraviolet (~374 nm) and visible (~525 nm) photoluminescence of pristine zinc oxide (ZnO) nanoparticles strongly depends on the concentration of H2O2 in water solution. Photoluminescence is quenched by up to 90 % at a 100 mM level of H2O2. The sensor constructed by immobilizing GOx on ZnO nanoparticles enabled glucose to be continuously monitored in the 10 mM to 130 mM concentration range, and the limit of detection is 10 mM. This enzymatic sensing scheme is supposed to be applicable to monitoring glucose in the food, beverage and fermentation industries. It has a wide scope in that it may be extended to numerous other substrate or enzyme activity assays based on the formation of H2O2, and of assays based on the consumption of H2O2 by peroxidases.

We report the application of ZnO nanoparticles (NPs) in a biosensor for monitoring glucose at a large concentration range. The biosensor is based on the indirect detection of hydrogen peroxide via the respective change of the UV and visible PL of ZnO NPs.

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Acknowledgments

This work is supported by EC FP-7 IRSES Grant #318520 “Development of nanotechnology based biosensors for agriculture” 2013–2016. Also, the support from the Swedish Research Council (VR) is acknowledged.

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Authors and Affiliations

  1. Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus, Academicheskaya St. 27, Minsk, 220072, Belarus

    Dzmitry Sodzel, Ekaterina Kolesneva, Lyudmila Dubovskaya & Igor Volotovski

  2. Department of Physics, Chemistry, and Biology (IFM), Linköping University, 583 81, Linköping, Sweden

    Volodymyr Khranovskyy, Martin O. Eriksson, Per-Olof Holtz & Rositsa Yakimova

  3. Biosensors & Bioelectronics Centre, Linköping University, 583 81, Linköping, Sweden

    Valerio Beni & Anthony P. F. Turner

  4. National Science Center FOTONIKA-LV, University of Latvia, 19, Raina Blvd, 1586, Riga, Latvia

    Roman Viter & Arnolds Ubelis

  5. Odessa National I.I. Mechnikov University, 42, Pastera, Odessa, 65026, Ukraine

    Roman Viter & Valentyn Smyntyna

  6. Institut Européen des Membranes, UMR5635 ENSCM UM CNRS, Université Montpellier, 2, Place Eugene Bataillon, 34095, Montpellier cedex 5, France

    Jean-Marc Janot, Mikhael Bechelany & Sebastien Balme

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  1. Dzmitry Sodzel
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  2. Volodymyr Khranovskyy
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Correspondence to Mikhael Bechelany.

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Sodzel, D., Khranovskyy, V., Beni, V. et al. Continuous sensing of hydrogen peroxide and glucose via quenching of the UV and visible luminescence of ZnO nanoparticles. Microchim Acta 182, 1819–1826 (2015). https://doi.org/10.1007/s00604-015-1493-9

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  • DOI: https://doi.org/10.1007/s00604-015-1493-9

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