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Quantum dot-modified paper-based assay for glucose screening

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Abstract

The article describes a simple optical assay for glucose. It is based on the use of paper spots loaded with colloidal CdSe/ZnS quantum dots (Q-dots) and the enzyme glucose oxidase (GOx). Circular paper sheets were uniformly loaded with Q-dots and then displayed strong fluorescence under a UV lamp (365 nm exCitation). The action of GOx causes the production of H2O2 which, after a typical exposure time of 20 min, causes fluorescence intensity to be quenched. To obtain a reading, the paper sheets were photographed under 365 nm excitation using a digital camera. Several parameters, including the amount of Q-dots, sample pH, and amount of GOx were optimized to maximize the response to glucose. The paper-based assay showed a sigmoidal-shaped response with respect to the glucose concentration in the 5–200 mg·dL−1 range (limit of detection of 5 μg·dL−1), demonstrating their potential use for biomedical applications.

A simple and inexpensive paper-based assay for glucose is reported. Following the optimzation of the amount of quantum dots, sample pH value, and amount of glucose oxidase (GOx), a sigmoidal response function was obtained that covers the 5–200 mg dL−1 glucose concentration range.

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Acknowledgments

This work was supported in part by he National Institute of Health through the National Institute of General Medical Sciences (2SC3GM081085), the Research Centers at Minority Institutions (G12MD007591), and the projects CTQ2013-48411-P (MINECO of Spain) and PEIC-2014-001-P (JCCM). Gema M. Durán thanks to MINECO of Spain for the predoctoral (BES-2011-045438) and stay (EEBB-I-14-08461) grants.

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Authors and Affiliations

  1. Department of Analytical Chemistry and Food Technology, University of Castilla - La Mancha, Avenue Camilo José Cela s/n, 13004, Ciudad Real, Spain

    Gema M. Durán & Ángel Ríos

  2. IRICA (Regional Institute of Applied Scientific Research), Avenue Camilo José Cela s/n, 13004, Ciudad Real, Spain

    Gema M. Durán

  3. Department of Chemistry, Clemson University, 219 Hunter Laboratories, Clemson, SC, 29634, USA

    Tomás E. Benavidez & Carlos D. García

Authors
  1. Gema M. Durán
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  2. Tomás E. Benavidez
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  3. Ángel Ríos
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  4. Carlos D. García
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Corresponding author

Correspondence to Carlos D. García.

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Durán, G.M., Benavidez, T.E., Ríos, Á. et al. Quantum dot-modified paper-based assay for glucose screening. Microchim Acta 183, 611–616 (2016). https://doi.org/10.1007/s00604-015-1685-3

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  • DOI: https://doi.org/10.1007/s00604-015-1685-3

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