Skip to main content
SpringerLink
Log in

Fermentation and evaluation of Klebsiella pneumoniae and K. oxytoca on the production of 2,3-butanediol

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

Klebsiella is one of the genera that has shown unbeatable production performance of 2,3-butanediol (2,3-BD), when compared to other microorganisms. In this study, two Klebsiella strains, K. pneumoniae (DSM 2026) and K. oxytoca (ATCC 43863), were selected and evaluated for 2,3-BD production by batch and fed-batch fermentations using glucose as a carbon source. Those strains’ morphologies, particularly their capsular structures, were analyzed by scanning electron microscopy (SEM). The maximum titers of 2,3-BD by K. pneumoniae and K. oxytoca during 10 h batch fermentation were 17.6 and 10.9 g L−1, respectively; in fed-batch cultivation, the strains showed the maximum titers of 50.9 and 34.1 g L−1, respectively. Although K. pneumoniae showed higher productivity, SEM showed that it secreted large amounts of capsular polysaccharide, increasing pathogenicity and hindering the separation of cells from the fermentation broth during downstream processing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Winfield ME (1945) The catalytic dehydration of 2,3-butanediol to 1,3-butadiene. J Council Sci Ind Res 18:412–423

    CAS  Google Scholar 

  2. Emerson RR, Flickinger MC, Tsao GT (1982) Kinetics of dehydration of aqueous 2,3-butanediol to methyl ethyl ketone. Ind Eng Chem Prod Res Dev 21:473–477

    Article  CAS  Google Scholar 

  3. Yu EKC, Saddler JN (1982) Power solvent production by Klebsiella pneumoniae grown on sugars present in wood hemicelluloses. Biotechnol Lett 4:121–126

    Article  CAS  Google Scholar 

  4. Garg SK, Jain A (1995) Fermentative production of 2,3-butanediol: a review. Bioresour Technol 51:103–109

    Article  CAS  Google Scholar 

  5. Harden A, Walpole GS (1906) 2,3-Butylene glycol fermentation by Aerobacter aerogenes. Proc Roy Soc B 77:399–405

    Article  CAS  Google Scholar 

  6. Syu MJ (2001) Biological production of 2,3-butanediol. Appl Microbiol Biotechnol 55:10–18

    Article  CAS  Google Scholar 

  7. Ji XJ, Huang H, Ouyang PK (2011) Microbial 2,3-butanediol production: a state-of-the-art review. Biotechnol Adv 29:351–364

    Article  CAS  Google Scholar 

  8. Celińska E, Grajek W (2009) Biotechnological production of 2,3-butanediol—current state and prospects. Biotechnol Adv 27:715–725

    Article  Google Scholar 

  9. Ma C, Wang A, Qin J, Li L, Ai X, Jiang T, Tang H, Xu P (2009) Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM. Appl Microbiol Biotechnol 82:49–57

    Article  CAS  Google Scholar 

  10. Barrett EL, Collins EB, Hall BJ, Matoi SH (1983) Production of 2,3-butylene glycol from whey by Klebsiella pneumonia and Enterobacter aerogenes. J Dairy Sci 66:2507–2514

    Article  CAS  Google Scholar 

  11. Jansen NB, Tsao FT (1983) Bioconversion of pentoses to 2,3-butanediol by Klebsiella pneumoniae. Adv Biochem Eng Biotechnol 27:85–99

    CAS  Google Scholar 

  12. Jansen NB, Flickinger MC, Tsao FT (1984) Production of 2,3-butanediol from d-xylose by Klebsiella oxytoca ATCC 8724. Biotechnol Bioeng 26:362–369

    Article  CAS  Google Scholar 

  13. Champluvier B, Decallonne J, Rouxhet PG (1989) Influence of sugar source (lactose, glucose, galactose) on 2,3-butanediol production by Klebsiella oxytoca NRRL-B199. Arch Microbiol 152:411–414

    Article  CAS  Google Scholar 

  14. Grover BP, Garg SK, Verma J (1990) Production of 2,3-butanediol from wood hydrolysate by Klebsiella pneumoniae. World J Microbiol Biotechnol 6:328–332

    Article  CAS  Google Scholar 

  15. Afschar AS, Bellgardt KH, Rossel CE, Czok A, Schaller K (1991) The production of 2,3-butanediol by fermentation of high test molasses. Appl Microbiol Biotechnol 34:582–585

    Article  CAS  Google Scholar 

  16. Frazer FR, McCaskey TA (1991) Effect of components of acid-hydrolysed hardwood on conversion of d-xylose to 2,3-butanediol by Klebsiella pneumoniae. Enzyme Microb Technol 13:110–115

    Article  CAS  Google Scholar 

  17. Cao N, Xia Y, Gong CS, Tsao GT (1997) Production of 2,3-butanediol from pretreated corn cob by Klebsiella oxytoca in the presence of fungal cellulose. Appl Biochem Biotechnol 63:129–139

    Article  Google Scholar 

  18. Ji XJ, Huang H, Du J, Zhu JG, Ren LJ, Li S, Nie ZK (2009) Development of an industrial medium for economical 2,3-butanediol production through co-fermentation of glucose and xylose by Klebsiella oxytoca. Bioresour Technol 100:5214–5218

    Article  CAS  Google Scholar 

  19. Biebl H, Zeng AP, Menzel K, Deckwer WD (1998) Fermentation of glycerol to 1,3-propanediol and 2,3-butanediol by Klebsiella pneumonia. Appl Microbiol Biotechnol 50:24–29

    Article  CAS  Google Scholar 

  20. da Silva GP, Mack M, Contiero J (2009) Glycerol: a promising and abundant carbon source for industrial microbiology. Biotechnol Adv 27:30–39

    Article  Google Scholar 

  21. Zheng Y, Zhang HY, Zhao L, Wei LJ, Ma XY, Wei DZ (2008) One-step production of 2,3-butanediol from starch by secretory over-expression of amylase in Klebsiella pneumoniae. J Chem Technol Biotechnol 83:1409–1412

    Article  CAS  Google Scholar 

  22. Ji XJ, Huang H, Du J, Zhu JG, Ren LJ, Hu N, Li S (2009) Enhanced 2,3-butanediol production by Klebsiella oxytoca using a two-stage agitation speed control strategy. Bioresour Technol 100:3410–3414

    Article  CAS  Google Scholar 

  23. Ji XJ, Huang H, Du J, Zhu JG, Ren LJ, Nie ZK, Du J, Li S (2010) Engineering Klebsiella oxytoca for efficient 2,3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene. Appl Microbiol Biotechnol 85:1751–1758

    Article  CAS  Google Scholar 

  24. Zhang L, Yang Y, Sun Jian, Shen Y, Wei D, Zhu J, Chu J (2010) Microbial production of 2,3-butanediol by mutagenized strain of Serratia marcescens H30. Bioresour Technol 101:1961–1967

    Article  CAS  Google Scholar 

  25. Cheng KK, Liu HJ, Liu DH (2005) Multiple growth inhibition of Klebsiella pneumoniae in 1,3-propanediol fermentation. Biotechnol Lett 27:19–22

    Article  CAS  Google Scholar 

  26. Woolley EM, George RM (1974) Ionization constants for water and for very weak organic acids in aqueous organic mixtures. J Sol Chem 3:119–126

    Article  CAS  Google Scholar 

  27. Riffat R, Krongthamchat K (2006) Specific methanogenic activity of halophilic and mixed cultures in saline wastewater. Int J Environ Sci Technol 2:291–299

    CAS  Google Scholar 

  28. Simoons-Smit AM, Verweij-van Vught AM, MacLaren DM (1986) The role of K antigen as virulence factors in Klebsiella. J Med Microbiol 21:133–137

    Article  CAS  Google Scholar 

  29. Lin MH, Hsu TL, Lin SY, Pan YJ, Jan JT, Wang JT, Khoo KH, Wu SH (2009) Phosphoproteomics of Klebsiella pneumoniae NTUH-K2044 reveals a tight link between tyrosine phosphorylation and virulence. Mol Cell Proteomics 8:2613–2623

    Article  CAS  Google Scholar 

  30. Guo NN, Zheng ZM, Mai YL, Liu HJ, Liu DH (2010) Consequence of cps mutation of Klebsiella pneumoniae on 1,3-propanediol fermentation. Appl Microbiol Biotechnol 86:701–707

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Mi Yeon Cho and Duk-Ki Kim for their technical assistance on cell cultures and analytical procedures. This work was supported by the Industrial Strategic Technology Development Program (No. 10035241) funded by the Ministry of Knowledge Economy (MKE, Korea).

Author information

Authors and Affiliations

  1. Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yusung-gu, Daejeon, 305-380, Republic of Korea

    Jung-Hee Cho, Chelladurai Rathnasingh, Hyohak Song, Hee Jong Lee & Doyoung Seung

  2. School of Chemical Engineering, Chonbuk National University, Jeon-ju, Jeonbuk, 561-756, Republic of Korea

    Bong-Woo Chung

Authors
  1. Jung-Hee Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chelladurai Rathnasingh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hyohak Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bong-Woo Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hee Jong Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Doyoung Seung
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hyohak Song.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cho, JH., Rathnasingh, C., Song, H. et al. Fermentation and evaluation of Klebsiella pneumoniae and K. oxytoca on the production of 2,3-butanediol. Bioprocess Biosyst Eng 35, 1081–1088 (2012). https://doi.org/10.1007/s00449-012-0691-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-012-0691-7

Keywords

Search

Navigation