Abstract
A simple and effective method of treatment of lignocellulosic material was used for the preparation of corn cob for the production of 2,3-butanediol byKlebsiella oxytoca ATCC 8724 in a simultaneous saccharification and fermentation process. During the treatment, lignin, and alkaline extractives were solubilized and separated from cellulose and hemicellulose fractions by dilute ammonia (10%) steeping. Hemicellulose was then hydrolyzed by dilute hydrochloric acid (1%, wJv) hydrolysis at 100°C at atmospheric pressure and separated from cellulose fraction. The remaining solid, with 90% of cellulose, was then used as the substrate. A butanediol concentration of 25 g/L and an ethanol concentration of 7 g/L were produced byK. oxytoca from 80 g/L of corn cob cellulose with a cellulase dosage of 8.5 IFPU/g corn cob cellulose after 72 h of SSF. With only dilute acid hydrolysis, a butanediol production rate of 0.21 g/L/h was obtained that is much lower than the case in which corn cob was treated with ammonia steeping prior to acid hydrolysis. The butanediol production rate for the latter was 0.36 g/L/h.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chemical Marketing Reporter (July 24,1995).
Garg, S. K. and Jain, A. (1995),Biosource Technol. 51, 103–109.
Jansen, N. B. and Tsao, G. T. (1983),Adv. Biochem. Eng. Biotechnol. 27, 85–100.
Jansen, N. B., Flickinger, M. C., and Tsao, G. T. (1984),Biotechnol. Bioeng. 26, 362–369.
Laube, V. M., Groleau, D., and Martin, S. M. (1984),Biotechnol. Lett. 6, 257–262.
Yu, E. K. C., Deschatelets, L., and Saddler, J. N. (1984),Biotech. Bioeng. Symp. 14, 341–352.
Dale, B. E. and Moreira, M. J. (1982),Biotech. Bioeng. Symp. 12, 31–43.
Grethlein, H. E. and Converse, A. O. (1991),Bioresource Technol. 36, 77–82
McMillan, J. D. (1994), inEnzymic Conversion of Biomass for Fuels Production. Himmel, M. E., Baker, J. O., and Overend, R. P. eds., ACS, Washington, DC, pp. 292–324.
Yoon, H. H., Wu, Z. W., and Lee, Y. Y. (1995),Appl. Biochem. Biotechnol. 51/52, 5–19.
Takagi, M., Abe, S., Suzuki, S., Emert, G. H., and Yata, N. (1977), inBioconversion Symposium Proceedings, IIT, Delhi, pp. 551–571.
Wright, J. D., Wyman, C. E., and Grohmann, K. (1988),Appl. Biochem. Biotechnol. 18, 75–90.
Sutcliffe, R. and Saddler, J. N. (1986),Biotechnol. Bioeng. Symp. 17, 749–762.
Ooshima, H., Burns, D. S., and Converse, A. O. (1990),Biotecnnol. Bioeng. 36, 446–452.
Elander, R. T. and Hsu, T. (1995),Appl. Biochem. Biotechnol. 51/52, 463–478.
Philippidis, G. P. and Smith, T. K. (1995),Appl. Biochem. Biotechnol. 51/52, 117–124.
Grohmann, K., Torget, R., and Himmel, M. (1985),Biotech. Bioeng. Symp. 15, 59–80.
Foley, K. M. (1978), Chemical properties, physical properties and uses of the Andersons' corncob products. The Andersons, pp. 425.
Ghosh, S. (1989),Biomass Handbook, Gordon and Breach Science Publishers, New York, NY, pp. 395,
Lawford, H. G. and Rousseau, J. D. (1992),Appl. Biochem. Biotechnol. 34/35, 185–204.
Lawford, H. G. and Rousseau, J. D. (1993),Appl. Biochem. Biotechnol. 39/40, 301–322.
Frazer, F. R. and McCaskey, T. A. (1991),Enzyme Micro. Technol. 13, 110–115.
Cao, N. J., Krishnan, M. S., Du, J. X., Gong, C. S., Ho, N. W. Y., Chen Z. D., and Tsao, G. T. (1996),Biotechnol Lett. 18, 1013–1018.
Chen, L. F. and Gong, C. S. (1985),J. Food Sci. 50, 226–228.
Burn, D. S., Ooshima, H., and Converse, A. O. (1989),Appl. Biochem. Biotech. 20/21, 79–94.
Saddler, J. N., Mes-Hartree, M., Yu, E. K. C., and Browell, H. H. (1983),Biotechnol. Bioeng. Symp. 13, 225–238.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cao, N., Xia, Y., Gong, C.S. et al. Production of 2,3- butanediol from pretreated corn cob byKlebsiella oxytoca in the presence of fungal cellulase. Appl Biochem Biotechnol 63, 129–139 (1997). https://doi.org/10.1007/BF02920419
Issue Date:
DOI: https://doi.org/10.1007/BF02920419