Abstract
2,3-Butanediol was produced at 12g/l (27% w/w yield) from the reducing sugars in wood hydrolysate byKlebsiella pneumoniae NRRL B-199. Ethanol was present at 4 g/l and acetoin at 0.4 g/l. Optimum conditions for a 48 h fermentation were pH 6.0, 30°C and 50 g reducing sugars/l. Adding 1% (w/v) malt extract to the medium-enhanced butanedlol production to 13.3 g/l (yield 29%) without changing ethanol production.
Résumé
On a produit 12 g/l de 2,3-butanediol (27% de rendement en poids) à partir des sucres réducteurs dans un hydrolysat de bois au moyen deKlebsiella pneumoniae NRRL B-199. L'éthanol était présent à raison de 4 g/l et l'acétoïne de 0.4 g/l. Les conditions optimum pour une fermentation de 48 h étainent les suivantes: pH 6.0, 30°C, et 50 g de sucres réducteurs par litre. L'ajout de 1% (p/v) d'extrait de malt au millieu accroît la production de butanediol jusqu'à 13.3 g/l (29% de rendement) sans changer la production d'éthanol.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Birkinshaw, J.H., Charles, J.H.W. &Clutterbuck, P. W. 1931 Estimation of butanediol by potassium periodate.Biochemical Journal 25, 1522–1539.
Flickinger, M.C. 1980 Current biological research in conversion of cellulosic carbohydrates into liquid fuels: how far have we come?Biotechnology and Bioengineering 22, 27–48.
Jansen, N.B., Flickinger, M.C. &Tsao, G.T. 1984 Production of 2,3-butanediol from D-xylose byKlebsiella oxytoca ATCC 8724.Biotechnology and Bioengineering 22, 362–369.
Joshi, A., Garg, S.K. &Verma, J. 1990 Production of ethanol from sugars in wood hydrolysate byFusarium oxysporum.World Journal of Microbiology and Biotechnology 6, 10–14.
Ledingham, G.A. & Neish, A.C. 1954 Fermentative production of 2,3-butanediol. InIndustrial Fermentations, eds. Underkofler, L. A. & Hickey, R.J., Vol. 2, pp. 27–94. Chemical Publishing Company.
Long, S.K. &Patrick, R. 1963 The present status of the 2,3-butylene glycol fermentation.Advances in Applied Microbiology 5, 135–155.
Miller, G.L. 1959 Use of dinitrosalicylic reagent for the determination of reducing sugars.Analytical Chemistry 31, 426–428.
Neish, A.C. &Ledingham, G.A. 1949 Production and properties of 2,3-butanediol—XXXII. 2,3-Butanediol fermentation at poised hydrogen ion concentration.Canadian Journal of Research 27, 694–704.
Perlman, D. 1944 Production of 2,3-butylene glycol from wood hydrolysates.Industrial Engineering Chemistry 36, 803–804.
Ranganna, S. 1977Manual of Analysis of Fruit and Vegetable Products. New Delhi: Tata/McGraw Hill
Reid, V.M. &Salmon, D.G. 1955 The determination of ethanol by a colorimetricAnalytical Structure 80, 704–708.
Saddler, J.N., Mes-Hartree, M., Yu, E.K.C. &Brownell, H.H. 1984 Enzymatic hydrolysis of various pretreated lignocellulosic substrates and the fermentation of the liberated sugars to ethanol and butanediol.Biotechnology and Bioengineering Symposium 13, 225–238.
Saddler, J.N., Yu, E.K.C., Mes-Hartree, M., Levitin, N. &Brownell, H.H. 1983 Utilization of enzymatically hydrolyzed wood hemi-celluloses by microorganisms for production of liquid fuels.Applied and Environmental Microbiology 45, 153–160.
Westerfeld, W.W. 1945 A colorimetric determination of blood acetoin.Journal of Biological Chemistry 161, 495–502.
Yu, E.K.C. &Saddler, J.N. 1982 Enhanced production of 2,3-butanediol byKlebsiella pneumoniae grown on high sugar concentrations in the presence of acetic acid.Applied and Environmental Microbiology 44, 777–784
Yu, E.K.C., Levitin, N. &Saddler, J.N. 1982 Production of 2,3-butanediol byKlebsiella pneumoniae grown on acid hydrolysed wood hemi-cellulose.Biotechnology Letters 4, 741–746.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Grover, B.P., Garg, S.K. & Verma, J. Production of 2,3-butanediol from wood hydrolysate byKlebsiella pneumoniae . World J Microbiol Biotechnol 6, 328–332 (1990). https://doi.org/10.1007/BF01201306
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01201306