Abstract
Enhanced 2,3-butanediol (BD) production was carried out by Klebsiella pneumoniae SDM. The nutritional requirements for BD production by K. pneumoniae SDM were optimized statistically in shake flask fermentations. Corn steep liquor powder and (NH4)2HPO4 were identified as the most significant factors by the two-level Plackett–Burman design. Steepest ascent experiments were applied to approach the optimal region of the two factors and a central composite design was employed to determine their optimal levels. The optimal medium was used to perform fed-batch fermentations with K. pneumoniae SDM. BD production was then studied in a 5-l bioreactor applying different fed-batch strategies, including pulse fed batch, constant feed rate fed batch, constant residual glucose concentration fed batch, and exponential fed batch. The maximum BD concentration of 150 g/l at 38 h with a diol productivity of 4.21 g/l h was obtained by the constant residual glucose concentration feeding strategy. To the best of our knowledge, these results were new records on BD fermentation.
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References
Afschar AS, Vaz Rossell CE, Jonas R, Quesada Chanto A, Schaller K (1993) Microbial production and downstream processing of 2,3-butanediol. J Biotechnol 27:317–329
Berbert-Molina MA, Sato S, Silveira MM (2001) Ammonium phosphate as a sole nutritional supplement for the fermentative production of 2,3-butanediol from sugar cane juice. Z Naturforsch 56:787–791
De Azeredo LAI, De Lima MB, Coelho RRR, Freire DMG (2006) A low-cost fermentation medium for thermophilic protease production by Streptomyces sp. 594 using feather meal and corn steep liquor. Curr Microbiol 53:335–339
de Mas C, Jansen NB, Tsao GT (1988) Production of optically active 2,3-butanediol by Bacillus polymyxa. Biotechnol Bioeng 31:366–377
Elibol M (2004) Optimization of medium composition for actinorhodin production by Streptomyces coelicolor A3 (2) with response surface methodology. Process Biochem 39:1057–1062
Ezeji TC, Qureshi N, Blaschek HP (2004) Acetone butanol ethanol (ABE) production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping. Appl Microbiol Biotechnol 63:653–658
Garg SK, Jain A (1995) Fermentative production of 2,3-butanediol: a review. Bioresour Technol 51:103–109
Hofvendahl K, Hahn-Hägerdal B (2000) Factors affecting the fermentative lactic acid production from renewable resources. Enzyme Microb Technol 26:87–107
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams & Wilkins, Baltimore, pp 181–235
Kaushik R, Saran S, Isar J, Saxena RK (2006) Statistical optimization of medium components and growth conditions by response surface methodology to enhance lipase production by Aspergillus carneus. J Mol Catal B Enzym 40:121–126
Kennedy M, Krouse D (1999) Strategies for improving fermentation medium performance: a review. J Ind Microbiol Biotechnol 23:456–475
Ko CL, Wang FS (2006) Run-to-run fed-batch optimization for protein production using recombinant Escherichia coli. Biochem Eng J 30:279–285
Kona RP, Qureshi N, Pai JS (2001) Production of glucose oxidase using Aspergillus niger and corn steep liquor. Bioresour Technol 78:123–126
Lee HK, Maddox IS (1986) Continuous production of 2,3-butanediol from whey permeate using Klebsiella pneumoniae immobilized in calcium alginate. Enzyme Microb Technol 8:409–411
Lee J, Lee SY, Park S, Middelberg AP (1999) Control of fed-batch fermentations. Biotechnol Adv 17:29–48
Li C, Bai J, Cai Z, Ouyang F (2002) Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology. J Biotechnol 93:27–34
Nor ZM, Tamer MI, Scharer JM, Moo-Young M, Jervis EJ (2001) Automated fed-batch culture of Kluyveromyces fragilis based on a novel method for on-line estimation of cell specific growth rate. Biochem Eng J 9:221–231
Parekh M, Formanek J, Blaschek HP (1999) Pilot-scale production of butanol by Clostridium beijerinckii BA101 using a low-cost fermentation medium based on corn steep water. Appl Microbiol Biotechnol 51:152–157
Qin JY, Xiao ZJ, Ma CQ, Xie NZ, Liu PH, Xu P (2006) Production of 2,3-butanediol by Klebsiella pneumoniae using glucose and ammonium phosphate. Chin J Chem Eng 14:132–136
Ramachandran KB, Goma G (1987) Effect of oxygen supply and dilution rate on the production of 2,3-butanediol in continuous bioreactor by Klebsiella pneumoniae. Enzyme Microb Technol 9:107–111
Reddy LVA, Wee YJ, Yun JS, Ryu HW (2008) Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett-Burman and response surface methodological approaches. Bioresour Technol 99:2242–2249
Rivas B, Moldes AB, Domínguez JM, Parajó JC (2004) Development of culture media containing spent yeast cells of Debaryomyces hansenii and corn steep liquor for lactic acid production with Lactobacillus rhamnosus. Int J Food Microbiol 97:93–98
Saha BC, Bothast RJ (1999) Production of 2,3-butanediol by newly isolated Enterobacter cloacae. Appl Microbiol Biotechnol 52:321–326
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
Silveira MM, Wisbeck E, Hoch I, Jonas R (2001) Production of glucose-fructose oxidoreductase and ethanol by Zymomonas mobilis ATCC 29191 in medium containing corn steep liquor as a source of vitamins. Appl Microbiol Biotechnol 55:442–445
Swift RJ, Karandikar A, Griffen AM, Punt PJ, Cees AMJJ, Hondel VD, Robson GD, Trinci APJ, Weibe MG (2000) The effect of organic nitrogen sources on recombinant glucoamylase production by Aspergillus niger in chemostat culture. Fungal Gen Biol 31:125–133
Syu MJ (2001) Biological production of 2,3-butanediol. Appl Microbiol Biotechnol 55:10–18
Syu MJ, Tsao GT, Choudhury T, Cooks RG (1993) Neural network modeling of butanediol fermentation monitored with tandem mass-spectrometry. Abstr Pap Am Chem Soc 205:1–BTEC
Xiu ZL, Zeng AP (2008) Present state and perspective of downstream processing of biologically produced 1,3-propanediol and 2,3-butanediol. Appl Microbiol Biotechnol 78:917–926
Yu EKC, Saddler JN (1983) Fed-batch approach to production of 2,3-butanediol by Klebsiella pneumoniae grown on high substrate concentrations. Appl Environ Microbiol 46:630–635
Zeng AP, Biebl H, Deckwer WD (1990) Effect of pH and acetic acid on the growth and 2,3-butanediol production of Enterobacter aerogenes in continuous culture. Appl Microbiol Biotechnol 33:485–489
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This work was supported by the grants by National Basic Research Program of China (2007CB707803) and Chinese National Programs for High-Technology Research and Development (2006AA02Z244 and 2006AA020101).
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Cuiqing Ma and Ailong Wang contributed equally to this work.
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Ma, C., Wang, A., Qin, J. et al. Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM. Appl Microbiol Biotechnol 82, 49–57 (2009). https://doi.org/10.1007/s00253-008-1732-7
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DOI: https://doi.org/10.1007/s00253-008-1732-7