Abstract
The ability of Klebsiella oxytoca NRRL-B199 to use either lactose or the mixture of glucose and galactose as substrate for the production of 2,3-butanediol was studied in batch fermentations with different conditions of aeration and pH. 2,3-butanediol was undetected, or present in minute concentration in the fermentation broths with lactose, while it was the main product from glucose+galactose with final concentrations of up to 18.8 g/l in media at pH 6.0. Under conditions optimal for 2,3-butanediol synthesis, when aeration limited growth, the rate of biomass growth was more tightly related to the aeration rate in lactose medium than in glucose+galactose medium. These relations suggest that the growth rate is very low on lactose but still considerable on glucose+galactose when aeration rate tends toward zero. Correspondingly, the metabolism is more oxidative in the former medium, yielding mainly acetate as product.
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Abbreviations
- CDW:
-
cell dry weight
References
Baronofsky JJ, Schreurs WJA, Kashket ER (1984) Uncoupling by acetic acid limits growth of and acetogenesis by Clostridium thermoaceticum. Appl Environ Microbiol 48:1134–1139
Dills SS, Apperson A, Schmidt MR, Saier MH (1980) Carbohydrate transport in bacteria. Microbiol Rev 44:385–418
Fond O, Jansen NB, Tsao GT (1985) A model of acetic acid and 2,3-butanediol inhibition of the growth and metabolism of Klebsiella oxytoca. Biotechnol Lett 7:727–732
Gekas V, Lopez-Leiva M (1985) Hydrolysis of lactose: a literature review. Process Biochem Feb 1985:2–11
Gottschalk G (1979) Bacterial metabolism. Springer, Berlin Heidelberg New York
Jansen NB, Tsao GT (1983) Bioconversion of pentoses to 2,3-butanediol by Klebsiella pneumoniae. Adv Biochem Eng Biotech 27:85–99
Jansen NB, Flickinger MC, Tsao GT (1984a) Production of 2,3-butanediol from d-xylose by Klebsiella oxytoca ATCC 8724. Biotechnol Bioeng 26:362–369
Jansen NB, Flickinger MC, Tsao GT (1984b) Application of bioenergetics to modelling the microbial conversion of d-xylose to 2,3-butanediol. Biotechnol Bioeng 26:573–582
Ledingham GA, Neish AC (1954) Fermentative production of 2,3-butanediol In: Underkofler LA (ed) Industrial fermentations, vol 2. Chemical Publications, New York, pp 27–93
Lee HK, Maddox IS (1984) Microbial production of 2,3-butanediol from whey permeate. Biotechnol Lett 6:815–818
Opdyke DLJ (1979) Monographs on fragrance raw materials. Food Cosmet Toxicol 17:509–511
Pirt SJ, Callow DS (1958) Exocellular product formation by microorganisms in continuous culture. I. Production of 2,3-butanediol by Aerobacter aerogenes in a single stage process. J Appl Bact 2:188–205
Queric MP, Hadjsassi A, Nonus M, Deschamps AM, Lebeault JM (1984) Liquid and dry fodder concentrate of lysine. Third Eur Congr Biotechnol, Munich, vol 2. Verlag Chemie, Weinheim, pp 533–538
Ramachandran KB, Goma G (1987) Effect of oxygen supply and dilution rate on the production of 2,3-butanediol in continuous bioreactor by Klebsiella pneumoniae. Enzyme Microb Technol 9:107–111
Severinsen SG (1979) Application des lactases. Alimentation 72:60–69
Speckman RA, Collins EB (1982) Microbial production of 2,3-butylene glycol from cheese whey. Appl Environ Microbiol 43:1216–1218
Teixeira de Mattos MJ, Tempest DW (1983) Metabolic and energetic aspects of the growth of Klebsiella aerogenes NCTC 418 on glucose in anaerobic chemostat culture. Arch Microbiol 134:80–85
Tran AV, Chambers RP (1987) The dehydration of fermentative 2,3-butanediol into methyl ethyl ketone. Biotechnol Bioeng 24:343–351
Van Huynh N, Decleire M, Voets AM, Motte JC, Monseur X (1986) Production of gluconic acid from whey hydrolysate by Gluconobacter oxydans. Process Biochem Feb 1986, 31–32
Yu EKC, Saddler JN (1983) Fed-batch approach to production of 2,3-butanediol by Klebsiella pneumoniae grown on high substrate concentrations. Appl Environ Microbiol 46:630–635
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Champluvier, B., Decallonne, J. & Rouxhet, P.G. Influence of sugar source (lactose, glucose, galactose) on 2,3-butanediol production by Klebsiella oxytoca NRRL-B199. Arch. Microbiol. 152, 411–414 (1989). https://doi.org/10.1007/BF00446920
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DOI: https://doi.org/10.1007/BF00446920